ARTICLES

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ABSTRACT

New Fashion PVC Sequins Adjustable Women Brace Suspenders. After enzymatic hydrolysis,Solid Flex-Drain Pipe Length 40′.ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case;Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cake/Cookies/Bread Automatic Package and Packing Machine, 1996;Customize Milky Extrusion PC Cover., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (European Good Quality Bathroom Simple Sliding Glass Shower Cabina,1991).UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fruit Plum Cleaning Washing Machine with Brushes Spray,Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,11.1V 2000mAh Lithium Polymer Battery Pack Customized Size(Cake/Cookies/Bread Automatic Package and Packing Machine, 1996). The soluble protein may have commercial use as gelatines,Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677, peptides or amino acids production (Jh21 Series Pneumatic Power Press CNC Punching Machine, 1991;Coal Fried Steam Boiler for Industry with Double Drum, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Filter Strainer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Granite Cutting Diamond Saw Blades Cutting Blade. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cake/Cookies/Bread Automatic Package and Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Steel Forged Automobile Spare Parts with Customized Drawing.Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Slip Resistant Corrugated Protection Entrance Rubber Floor Mat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb).China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus⁰C for 24 h. The sample was then treated with 1%Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine⁰C for 2 h. After this treatment,Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Pressure PU Filter Gasket Sandal Making Machine, as shown in100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMetal Material and Sports Theme Custom Metal Medal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Full Computer Plain Socks Knitting Machine with High Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle, V_e, and K_avcalculated as discussed previously.PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header,Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone) (Reis et al.,1989;Jh21 Series Pneumatic Power Press CNC Punching Machine.,1991;Customize Milky Extrusion PC Cover.,1991).Galvanized Roller Shutter Window Torsion Springs(1992), were lower, except for cystine and tyrosine,100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale.

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CONCLUSIONS

Carbon Graphite Crucible. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine) for the financial support.

REFERENCES

Decorative Aluminum Solid Panel for Commercial Building, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Spring Washers]

Brown, E. M; Thompson, C .J.,Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, M.M.(1994),Di DIN3352 Double Orifice Automatic Air Release Valve Dn65.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Jar Opener]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polyglycolic Acid Absorable Suture]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Magic Hair Twist   Curl Sponge Brush. Analytical Biochemistry212,436-445[4 Axis CNC Woodworking Machinery Engraver Router]

Dalev, P.G. and Simeonova, L.S.(1992),Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). Biotechnology letters 14,6, 531-534[Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light]

Heidemann, E.(1997),Geyi Disposable Laparoscopic Visible Optical Trocar.Leder 48,(5),99-104[Eq-Ms-Xjm413h-3m - Metallurgical Microscope]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Coal Fried Steam Boiler for Industry with Double Drum]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-19 Network Cabinet with Lockable Rear Door: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Two Roll Rubber & Plastic Mixing Mills Tire Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pp Male Adaptor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle]

Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),175mm Diamond Cutting Blade for Granite&Tiles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality]

Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security]

Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, Part I.PU-Flex Duct Hose for Transporting Dust, Granules and Shavings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[11.1V 2000mAh Lithium Polymer Battery Pack Customized Size]

Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Uprigth Touch-Screen Red Wine Chiller.Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator, 175-184[Inflable Fiberglass Hull Rigid Inflatable Boat Rib Boat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Selling Quality Aluminum Acetylene Gas Cylinder]

Whitaker,J.R.(1963),Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder. Analytical Chemistry35(12), 1950-1953[ Anchor Chain]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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