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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Bearing with Housing Bearing / Pillow Block Bearing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800

ABSTRACT

The Most Competitive Sodium Gluconate for Water Reducer. After enzymatic hydrolysis,Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles.Electric Magic Hot Hair Styling Curling Iron with Brushes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Melt Pressure Transducer (PT133);Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine, 1996;Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662,1991).Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck,Durable Aluminum Flag Pole with Dome Top Tangle Free Finial,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Marathon Medal for Marathon Medallion(Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine, 1996). The soluble protein may have commercial use as gelatines,Price of a Retail Supermarket Push Cart for Sale, peptides or amino acids production (Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder, 1991;Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hotel Steel Digital Room Safes with Large LED Display., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Biogas/Methane Purification System/Desulfurization/Scrubber Tower. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line.Popular Custom School Uniform Woven Badge Patch with Merrow Border-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Used/Second Hand 120mpm Continous Galvanizing Line/Cgi. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUnisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Stainless Steel Expanded Metal Mesh, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Popular Lovely Animal Design Inflatable Slide for Kids, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage) for the financial support.

REFERENCES

Asco 353 Series 3/4 Pulse Valve Solenoid Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.sajoo-switch.com]

Brown, E. M; Thompson, C .J.,China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, M.M.(1994),Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Purple UL 1095 16AWG Electronic Lead Wire. Analytical Biochemistry212,436-445[Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry]

Dalev, P.G. and Simeonova, L.S.(1992),Brushed Anodizing Aluminum Extrusion Profile for LED. Biotechnology letters 14,6, 531-534[Price of a Retail Supermarket Push Cart for Sale]

Heidemann, E.(1997),PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps.Leder 48,(5),99-104[Auto Shut Off Valve For Water Leak]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Enamel Cast Iron Mini Casserole Cast Iron Cocotte]

Eyes- Caring LED Night Light for Nursing Reading with Soft Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),S235jo S235jr Steel Pipe Tube Shs Rhs.Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing. USP/SP[Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pre-Seasoned Cast Iron Mini Oval Casserole Pot: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Key To Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine]

Stainless Steel Square Wire Mesh Netting with SGS, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process]

Stainless Steel Square Wire Mesh Netting with SGS,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662]

Stainless Steel Square Wire Mesh Netting with SGS, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, Part I.Outdoor Garden Save Energy with Solar Flame Wall Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ API 5L Spec 5L Steel Pipe]

CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag.Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length, 175-184[Prestressed Precast Concrete Steel Wire]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Screen Printing Materials and Equipments Screen Printing Frames]

Whitaker,J.R.(1963),Non Stick Silicone Coated Fiberglass Cloth. Analytical Chemistry35(12), 1950-1953[Women Fashion Winter V Neck Long Sleeve Clothes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Black Phosphated Bugle Head Csk Head Drywall ScrewAuthor for correspondence