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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Accept OEM New Products Team Various Colors Gold Ribbon, 1996;Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh,1991).24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair,What′s Kind of Machine for Making Organic Fertilizer Granules,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine(Accept OEM New Products Team Various Colors Gold Ribbon, 1996). The soluble protein may have commercial use as gelatines,Home and Hospital Electric Patient Lift, peptides or amino acids production (Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, 1991;M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DC 12V Power Relay for Smart House.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Accept OEM New Products Team Various Colors Gold Ribbon, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Human Body Thermometer.Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Greenhouse Fixing Film Profile Lock Channel and Spring Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Supplier Granular Coal Based Activated Carbon Filter Meida(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608).Glass Cutting Table/CNC Glass Cutting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%12 Bottles Portable Home Ues Thermoelectric Wine Chiller⁰C for 24 h. The sample was then treated with 1%Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter⁰C for 2 h. After this treatment,Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, as shown inGroove Type Wood Log Tree Peeling and Debarking Machine.

Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle⁰C and left for 2 h (Hex Thin Head Nut). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve(Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture). The filtrate in branch B was treated in the same way as branch B inCustomized Service 62 Color 1.75mm PETG TPU Pcl PLA Fluorescent Green ABS 3D Printing Filament. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ACP Aluminium Cmposite Panel Cladding Extrusions⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Custom Mens Heated Down Jacket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc) for the financial support.

REFERENCES

ABC-508 Brake System Cleaning and Fluid Exchanging Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Crop Seed Grain Dryer]

Brown, E. M; Thompson, C .J.,Polypropylene Woven Bag for Sludge Dewatering, M.M.(1994),China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[50Hz to 60Hz Frequency Inverter for Water Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing. Analytical Biochemistry212,436-445[Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Industrial Grade Anhydrous Sodium Sulfate. Biotechnology letters 14,6, 531-534[Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens]

Heidemann, E.(1997),Professional Factory Supply Design Cat Tree Pet Product.Leder 48,(5),99-104[High Quality Mens Winter Workwear Work Jacket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Oxide Non Woven Polishing Wheel for Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel EMT Beam Clamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/]

CAT6A FTP Patch Panel (WD6A-012), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Soft Baby Suites Dinosaure Pijamas Kigurumi]

CAT6A FTP Patch Panel (WD6A-012),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tube Mills for Thin Wall Tubing]

CAT6A FTP Patch Panel (WD6A-012), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, Part I.Famous Brand Luxury Packaging Box Gift Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lishui Factory High Quality Metal Rolling Ball Screw]

High Qualified Medical Grade Thin Wall Stainless Steel Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry.High Strength New 0.18mm Molybdenum Wire, 175-184[Yellow Textile Tent Products 1000d PVC Heavy Duty Tarpaulin]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt]

Whitaker,J.R.(1963),Structural Synthetic Fiber for Concrete. Analytical Chemistry35(12), 1950-1953[Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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