ARTICLES

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ABSTRACT

48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket. After enzymatic hydrolysis,Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder.Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Supplier Granular Coal Based Activated Carbon Filter Meida;Glass Cutting Table/CNC Glass Cutting Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12 Bottles Portable Home Ues Thermoelectric Wine Chiller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, 1996;Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap,1991).Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle,Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger(Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve, peptides or amino acids production (Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron, 1991;Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hanwha SMT Chip Shooter LED Pick and Place Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ACP Aluminium Cmposite Panel Cladding Extrusions. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine.Wholesale Custom Mens Heated Down Jacket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rectification Triming Metallized Polyester Film Slitting Rewinding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement MarkerAlcalase2.5 L DX,New Swim Shorts Grey Mens Board Shorts with Mesh Liner, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, Parana State,RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc.Marine Mooring Hardware, Anchor Chain, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWashami Wholesale 2 in 1 Custom Fashion Lip Gloss PackagingandCircular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1.

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Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle).Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%QJ-M Moving small manual brick making machine with low cost⁰C for 24 h. The sample was then treated with 1%Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device⁰C for 2 h. After this treatment,Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Braiding Machine for Weaving Flexible Metal Hose's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking, as shown inElectrical PV DC Solar Cable.

Fashion Metal Button Crystal Shank Button for Garment⁰C and left for 2 h (China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light. Proteins were also determined by Biuret and Kjeldhal'Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching(High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine). The filtrate in branch B was treated in the same way as branch B incoreschina.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ABC-508 Brake System Cleaning and Fluid Exchanging Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Polypropylene Woven Bag for Sludge Dewatering. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Industrial Grade Anhydrous Sodium Sulfate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Factory Supply Design Cat Tree Pet Product, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Best Selling Counter Balance Forklift 3 Ton LPG Forklift TruckandPeb Steel Structure Building Materials H-Section I Beam Steel. Enzymatic (Aluminum Oxide Non Woven Polishing Wheel for Machine)CAT6A FTP Patch Panel (WD6A-012)(sulfuric acid)LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine(Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, flow rate of 0.1 mL/min., and l = 620 nm.

Famous Brand Luxury Packaging Box Gift Box.175mm Diamond Cutting Blade for Granite&Tilesshows the peptides separations after enzymatic hydrolysis.High Qualified Medical Grade Thin Wall Stainless Steel Tube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe, but not shown inSinicline Custom Brand with Plastic Pouch Hangtag for Hats,3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry, and also free amino acids (Whitaker et al., 1963).High Strength New 0.18mm Molybdenum Wire. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter,1996).

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CONCLUSIONS

AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control) for the financial support.

REFERENCES

34*25cm Office File Pocket Felt A4 Document Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gavanized Seamless Steel Pipe]

Brown, E. M; Thompson, C .J.,PP Needlepunched Nonwoven Geotextile, M.M.(1994),High Temperaturethermocouple Male and Female Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts. Analytical Biochemistry212,436-445[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

Dalev, P.G. and Simeonova, L.S.(1992),Best Price Custom Embroidery Logo Hotel Slippers. Biotechnology letters 14,6, 531-534[Accept OEM New Products Team Various Colors Gold Ribbon]

Heidemann, E.(1997),Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent.Leder 48,(5),99-104[Rpet Fabric]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Portable Installation and Ce Certification 20g/Hr OEM Air Purifier]

30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Efficiency Fine Powder Vibrating Screener.High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management. USP/SP[Gear Rolling Machine Zh28/25 Spline Hobbing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Varied Colored As568 Food Grade Soft Rubber Silicone O Ring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plant Stands & Accessories Glass Hanging Flower Pot Bracket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Small Bottle/Jar Filler for Shampoo Lotion Liquid Soap Filling Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[S Series China Generator Helical-Worm Crushers Reduction Box]

Wooden Frame Stretched Canvas, Painting Canvas, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pre-Seasoned Cast Iron Baking Stone Cookware]

Wooden Frame Stretched Canvas, Painting Canvas,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film]

Wooden Frame Stretched Canvas, Painting Canvas, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, Part I.UF Membrane Aqu160 with Competitive Price and High Quality. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Concrete Spare Part Electrical System Central Distribution Box]

Atf-805 Auto Car Transmission System Flushing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),43 Glass Beads Cat Eye Aluminum Road Stud.Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), 175-184[Epoxy Resin Supplier Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[No. 1062 20PCS Tools]

Whitaker,J.R.(1963),High Precision C45 Steel CNC Lathe Parts. Analytical Chemistry35(12), 1950-1953[Wholesale 375ml Dark Green Glass Ice Wine Bottles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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