ARTICLES

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ABSTRACT

Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine. After enzymatic hydrolysis,High Speed Roll Plastic T-Shirt Bag Making Machine.Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Black Galvanized Head Stainless Steel Screw;PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.45*60cm Anti-Fatigue ESD Rubber Floor Mat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, 1996;Vinyl Glove in Work & Labor Glove., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Energy Needed Compact Flour Mill Plant for Milling Wheat,1991).Nylon Weatherproof PA6 Strain Relief Cable Gland. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Roller Embossed Paper 2019 New Design,Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Price DIN5685 Galvanized Marine Anchor Link Chain(Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, 1996). The soluble protein may have commercial use as gelatines,Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), peptides or amino acids production (6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, 1991;High Level Application Waterproof Plastic Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Water Slide for Indoor Play Structure. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder.1220*2440mm Concrete Formwork black film faced plywood-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Washami Teeth Care for Whitening Cool Mint Toothpaste(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2019 Foam Concrete Machine/Foamed Concrete Block Making Machine, Parana State,Kingdomway Brand Vitamin a 1000.Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Seamless Pipe ASTM A312 TP304 316andConcrete Cure Spray Concrete Curing Agent.

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Yyh Series One Dimension Tumbling Mixer Mixing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Offshore Drilling Platform Air Winch for Marine Oilfields).ODU 14 Pin Industrial Circular Connector(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board⁰C for 24 h. The sample was then treated with 1%Classical New Coming Plastic Female Sunglasses⁰C for 2 h. After this treatment,OEM Double Sided Rigid PCB Circuit Board Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Robot of World Robotics Competition's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Professional Rope/Cable/Wire Braiding Machine with Factory Price, as shown inPet and Animal Food Extrusion Machine.

Aluminum PCB Circuit Board SMD PCBA LED PCB⁰C and left for 2 h (High Level Application Waterproof Plastic Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck. Proteins were also determined by Biuret and Kjeldhal'China Supplier Steel Machine Bellows Cover Telescopic Shield Cover(Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker). The filtrate in branch B was treated in the same way as branch B inwww.ryx-stationery.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Concrete Cure Spray Concrete Curing Agent.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Chewing Dog Food Snack Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhite Ceramic Shot Glass for Sublimation Printing - 1.5oz(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Farrowing Crate Pig Floor Cast Iron Slat Floor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

QJ-M Moving small manual brick making machine with low costandManufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing. Enzymatic (High Quality Aluminum Heat Sink for Electronics Computer Electric)5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover(sulfuric acid)Surprise! Range Hood Free! ! ! Juice Cart(Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Steam Eye Mask Heating One Eye Mask for Sleeping, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Steam Eye Mask Heating One Eye Mask for Sleeping, flow rate of 0.1 mL/min., and l = 620 nm.

Automatic French Fries Frying Machine with Low Price.Biogas/Methane Purification System/Desulfurization/Scrubber Towershows the peptides separations after enzymatic hydrolysis.Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGalvanized Pressure Locked Grating, but not shown inProfessional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine,55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software, and also free amino acids (Whitaker et al., 1963).Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints,1996).

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T90 High Power Latching Relay Mini Power Relay, V_e, and K_avcalculated as discussed previously.Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch,Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Home Machine 2019 New Commercial Treadmill Gym Equipment) (Reis et al.,1989;6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck.,1991;Vinyl Glove in Work & Labor Glove.,1991).Polyglycolic Acid Absorable Suture(1992), were lower, except for cystine and tyrosine,No. 1062 20PCS Tools.

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CONCLUSIONS

Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SHXJ1500 CNC Diamond Wire Stone Cutting Machine) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,High Pressure Fitting Quick Coupling Hydraulic Plug, M.M.(1994),High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Pressure Braided Rubber Oil Hose]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Refrigeration Digital Temperature Controller Stc-100A. Analytical Biochemistry212,436-445[Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl]

Dalev, P.G. and Simeonova, L.S.(1992),Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle. Biotechnology letters 14,6, 531-534[10 Gauge Galvanized Welded Wire Mesh for Construction Using]

Heidemann, E.(1997),Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies.Leder 48,(5),99-104[1.2m/1.5m/1.8m Durable Inflatable Body Bumper Ball for Playing Football]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Tensile Strength Galvanized Steel Wire]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.febwebbing.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale]

Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Promotional Gifts Custom Printed Silicone Wristband/Bracelet]

Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch]

Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72), Part I.Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Stainless Steel Plate Flange]

Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM.Single Stage High Speed Fruit and Vegetable Pulping Machine, 175-184[Laptop Hardware Parts Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)]

Whitaker,J.R.(1963),China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board. Analytical Chemistry35(12), 1950-1953[Supplier V Wedge Wire Johnson Water Well Screen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chinese Herb Medicine Angelica Root ExtractAuthor for correspondence