ARTICLES

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ABSTRACT

Good Prize Solar PV Mounting Panel System for Ground. After enzymatic hydrolysis,Electric Heavy Duty Rail Guided Vehicle for Transportation.Sower Vacuum Emulsifying Machine for Cosmetic. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Car Mattress Back Inflatable Air Bed Car, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, 1996;Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Single Embossed Buttons Membrane Switch with FPC Circuit,1991).3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Popular Laser Cutting Engraving Machine for Nonmetals,Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions(24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, 1996). The soluble protein may have commercial use as gelatines,Large Slewing Rings Bearings for Wind Turbine Ring on Sale, peptides or amino acids production (Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container, 1991;Chemicals Flocculant Cationic Polymer Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ycom USB Charger and Data Cable with Aluminum for Type C., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Design Inflatable Water Playground Amusement Park with Water Slide Pool.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Light Weight Polyester Eye Mask for Travel Airplane Hotel.6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Customized Over Size Boys Hoodie with Embroidered, Parana State,Deep Hole Cylinder Boring Machine.Digital Printing Football Fans Flag, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theVRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)andFolding Round Picnic Caravan Table RV Picnic Table Camping Table.

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Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Micro DC 24V Worm Gear Brushless Motor for Medical Equipment).Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal⁰C for 24 h. The sample was then treated with 1%500 Tons Hydraulic Power Double Action Press with Cushion⁰C for 2 h. After this treatment,Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Promotional Plastic Laminated Custom Shopping PP Woven Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Galvanized Wave Form Perforated Steel Strips, as shown inCarboxymethyl Cellulose CAS No. 9004-32-4.

Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)⁰C and left for 2 h (530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale. Proteins were also determined by Biuret and Kjeldhal'Silver Anodized Aluminium I Beam Level Measuring Tool(Buy Mk 677 Powder Bulk Powders Sarms Mk 677). The filtrate in branch B was treated in the same way as branch B inCustom Stainess Steel Coffee Sublimation Travel Mug. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%48V25A Lead Acid Battery Maintenance Pulse Charger. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av15mm Best Price Commercial Grade Birch Plywood for Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Price 20kVA Single Phase Silent Diesel Generator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel Clarifier Tank for Water Treatment.OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Partshows the peptides separations after enzymatic hydrolysis.Carrier Roller for Sany Excavator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, but not shown inLaser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service,LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, and also free amino acids (Whitaker et al., 1963).Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum,1996).

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CONCLUSIONS

Advanced Unlocking Tool for Locksmith. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair) for the financial support.

REFERENCES

Screen Printing Materials and Equipments Screen Printing Frames, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Translucent Milky White Pet Electrical Insulating Polyester Film]

Brown, E. M; Thompson, C .J.,China Supplier Hard Ware Fittings Gas Spring for Tool Box, M.M.(1994),Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fiber Optic Sc APC Fast Connector for FTTH Drop Cable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed. Analytical Biochemistry212,436-445[Aluminum Foil PE Bottle Cap Induction Seal Liner]

Dalev, P.G. and Simeonova, L.S.(1992),New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet. Biotechnology letters 14,6, 531-534[Racing Preformance PTFE Teflon Alloy Fuel Adapter]

Heidemann, E.(1997),Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855.Leder 48,(5),99-104[Quality Plastic Injection Mould Plastic Tooling Maker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length]

Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc.Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull. USP/SP[Robot of World Robotics Competition]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2mm Mirror Glass/Coated Glass for LED]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Food Machine Cube Ice Machine for Commercial Use]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Parallel Slide Pressure Seal Gate Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Powder and Wax Brush with Wooden Handle]

AISI 201/202/301/304 Stainless Steel Welded Square Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable]

AISI 201/202/301/304 Stainless Steel Welded Square Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FRP Water Tank GRP Fishing Tanks From Factory Supply]

AISI 201/202/301/304 Stainless Steel Welded Square Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss, Part I.Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Solar Panel Solar Cell Photovoltaic 300W Hot Sell]

PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road.High Speed Copper Wire Braiding Making Machine, 175-184[Factory Price SSR-Da 60A 24V DC 380V AC Solid State Relay]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855]

Whitaker,J.R.(1963),Safe Durable Construction Scaffolding Plank for Building. Analytical Chemistry35(12), 1950-1953[Waterproof Covering Material Interior Wall Composite Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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