ARTICLES

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ABSTRACT

Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001). After enzymatic hydrolysis,6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck.High Level Application Waterproof Plastic Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine;Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Water Slide for Indoor Play Structure, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, 1996;1220*2440mm Concrete Formwork black film faced plywood., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Washami Teeth Care for Whitening Cool Mint Toothpaste,1991).Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine,SHXJ1500 CNC Diamond Wire Stone Cutting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag(Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, 1996). The soluble protein may have commercial use as gelatines,2019 Foam Concrete Machine/Foamed Concrete Block Making Machine, peptides or amino acids production (Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, 1991;Yyh Series One Dimension Tumbling Mixer Mixing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ODU 14 Pin Industrial Circular Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Classical New Coming Plastic Female Sunglasses. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Double Sided Rigid PCB Circuit Board Manufacturer.Robot of World Robotics Competition-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Rope/Cable/Wire Braiding Machine with Factory Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Concrete Cure Spray Concrete Curing Agent, Parana State,Factory Price DIN5685 Galvanized Marine Anchor Link Chain.Automatic Chewing Dog Food Snack Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWooden Grain Perforated Panel for Metal Mesh Curtain WallandHome Furniture Set Restaurant King Throne Wedding Banquet Dining Chair.

316L)

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card, pH 3.25., mobile phase B: buffer solution of 0.01 mol/T90 High Power Latching Relay Mini Power Relay, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminum Mould Shoe Mould Making CNC Milling Router Machine.High Temperaturethermocouple Male and Female Plugshows the peptides separations after enzymatic hydrolysis.High Pressure Fitting Quick Coupling Hydraulic Plug(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle, but not shown inElectrical PV DC Solar Cable,High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, and also free amino acids (Whitaker et al., 1963).Refrigeration Digital Temperature Controller Stc-100A. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder,1996).

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CONCLUSIONS

Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck) for the financial support.

REFERENCES

Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Silicone Reusable Food Storage Bag China Manufacturer]

Brown, E. M; Thompson, C .J.,Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, M.M.(1994),Single Stage High Speed Fruit and Vegetable Pulping Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[20FT New Shipping Container 40FT Dry Cargo Container]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board. Analytical Biochemistry212,436-445[High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services. Biotechnology letters 14,6, 531-534[Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price]

Heidemann, E.(1997),Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads.Leder 48,(5),99-104[China Garden Hose]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2ton R Type Electric Wire Rope Hoist with Single Speed]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Movable Partition Walls for Room Division, Hotel and Shopping Mall: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Foil Mylar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

Carboxy Terminal Collagen Crosslinks Elisa Kits, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Various Colors Nylon and Stainless Steel Kitchen Tools-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Accept OEM New Products Team Various Colors Gold Ribbon]

Carboxy Terminal Collagen Crosslinks Elisa Kits,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Portable Installation and Ce Certification 20g/Hr OEM Air Purifier]

Carboxy Terminal Collagen Crosslinks Elisa Kits, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:355nm UV Laser Marking Equipment for Crystal Materials, Part I.Metal Separating Gold Brass Zippers for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gear Rolling Machine Zh28/25 Spline Hobbing Machine]

Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Refrigeration Digital Thermometer.BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L), 175-184[www.taibaoink.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Varied Colored As568 Food Grade Soft Rubber Silicone O Ring]

Whitaker,J.R.(1963),Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making. Analytical Chemistry35(12), 1950-1953[Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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