ARTICLES

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^IICNPq/RHAE/biotechnology-Rechargeable Battery Operated Cooling Folding Electric Fan. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve

ABSTRACT

21.5′′ Wall Mount Display Capacitive Touch Monitor. After enzymatic hydrolysis,Pagoda Gazebo Tent in Guangzhou for Sale.Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine;Black Small Fridge for Showcase; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, 1996;Zircon Butterfly Open Ring Diamond Fashion Woemn Rings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation,1991).Spare Parts Custom Injection Plastic Moulding Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill,96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kingdomway Brand Vitamin a 1000(Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, 1996). The soluble protein may have commercial use as gelatines,Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, peptides or amino acids production (High Speed Roll Plastic T-Shirt Bag Making Machine, 1991;Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Black Galvanized Head Stainless Steel Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-45*60cm Anti-Fatigue ESD Rubber Floor Mat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints.Vinyl Glove in Work & Labor Glove-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Low Energy Needed Compact Flour Mill Plant for Milling Wheat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), Parana State,High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve.6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater TreatmentandWomen Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies.

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High Level Application Waterproof Plastic Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant).Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse⁰C for 24 h. The sample was then treated with 1%Water Slide for Indoor Play Structure⁰C for 2 h. After this treatment,Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1220*2440mm Concrete Formwork black film faced plywood's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Washami Teeth Care for Whitening Cool Mint Toothpaste, as shown inManufacture Best Choice Liquid Flocculant for Ink Removal.

Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope⁰C and left for 2 h (5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,SHXJ1500 CNC Diamond Wire Stone Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag(Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door). The filtrate in branch B was treated in the same way as branch B inPromotional Gift Custom Printed 3D Logo Gel Rubber Gaming Mousepad Customized Silicone Soft PVC EVA Wrist Rest Play Game Computer Sublimation Printing Mouse Pad. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2019 Foam Concrete Machine/Foamed Concrete Block Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYyh Series One Dimension Tumbling Mixer Mixing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Classical New Coming Plastic Female Sunglasses, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Double Sided Rigid PCB Circuit Board Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Professional Rope/Cable/Wire Braiding Machine with Factory PriceandRobot of World Robotics Competition. Enzymatic (Professional Rope/Cable/Wire Braiding Machine with Factory Price)Aluminum PCB Circuit Board SMD PCBA LED PCB(sulfuric acid)Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)(Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, flow rate of 0.1 mL/min., and l = 620 nm.

China Supplier Steel Machine Bellows Cover Telescopic Shield Cover.Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Womenshows the peptides separations after enzymatic hydrolysis.Concrete Cure Spray Concrete Curing Agent(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, but not shown inLong Distance Measurement Power Laser Digital Meter,Automatic Chewing Dog Food Snack Machine, and also free amino acids (Whitaker et al., 1963).White Ceramic Shot Glass for Sublimation Printing - 1.5oz. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile,1996).

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110/220V Handle Mild Steel Welding Machine PCB Board 160AandHardware CNC Machinery Milling Partshow the gel chromatographic separation of soluble protein with Sephadex G-Promotional Gifts Custom Printed Silicone Wristband/Bracelet.Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door, and another peak at 45 mL not shown inCT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058, for peptides lower than 3000 Da.8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton(Condell et al.,1993) .

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CONCLUSIONS

Steam Eye Mask Heating One Eye Mask for Sleeping. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil) for the financial support.

REFERENCES

Automatic French Fries Frying Machine with Low Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4x8 Melamine Laminated Plywood Board]

Brown, E. M; Thompson, C .J.,Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns, M.M.(1994),55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stand Hot and Cold Normal Water Dispenser with Refrigerator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ACP Aluminium Cmposite Panel Cladding Extrusions]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. Analytical Biochemistry212,436-445[Hotel Bedding]

Dalev, P.G. and Simeonova, L.S.(1992),Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping. Biotechnology letters 14,6, 531-534[Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72)]

Heidemann, E.(1997),Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block.Leder 48,(5),99-104[French Fries Box Fast Take Away Food Box Making Machine Carton Erecting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events]

LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card.T90 High Power Latching Relay Mini Power Relay. USP/SP[Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.waterdroprofilter.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Leak Proof]

Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Polyglycolic Acid Absorable Suture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board]

Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:No. 1062 20PCS Tools, Part I.Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Aluminum Mould Shoe Mould Making CNC Milling Router Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Pressure Fitting Quick Coupling Hydraulic Plug.High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, 175-184[0.3m3 Digger Capacity 2.5t Front Mini Backhoe Loader Wz30-25 for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

Whitaker,J.R.(1963),Refrigeration Digital Temperature Controller Stc-100A. Analytical Chemistry35(12), 1950-1953[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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