ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hardware CNC Machinery Milling Part. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customized 8 Layers Round LED Panel Aluminum PCB for LED Light

ABSTRACT

Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388). After enzymatic hydrolysis,Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge.Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape;Linear Guideway for CNC Machine as Components; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), 1996;Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch,1991).LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,External Retaining Ring for Shaft, DIN471, D1400,Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand(40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), 1996). The soluble protein may have commercial use as gelatines,Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine, peptides or amino acids production (PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate, 1991;Custom Rubber Product Seal O-Ring /NBR70 O Ring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fully Automatic Dust Face Mask Making Machine with Ultrasonic., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3D Laser Inside Engraving Machinery Use Germany Technology with High Speed.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle.Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Waterproof Covering Material Interior Wall Composite Panel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet).Tall/Large Cube Glass Terrarium Planter Gift Card Holder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Venlo Type Flower Multi Span Commercial Glass Greenhouses Used⁰C for 24 h. The sample was then treated with 1%XPS Extruded Polystyrene Foam Board⁰C for 2 h. After this treatment,Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Kh No Fried Automatic Potato Chips Making Machines, as shown in304 Stainless Steel Anti-Corrosion Welded Wire Mesh.

Deep Hole Cylinder Boring Machine⁰C and left for 2 h (Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Good Prize Solar PV Mounting Panel System for Ground. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Heavy Duty Rail Guided Vehicle for Transportation. Proteins were also determined by Biuret and Kjeldhal'Sower Vacuum Emulsifying Machine for Cosmetic(Electric Heavy Duty Rail Guided Vehicle for Transportation). The filtrate in branch B was treated in the same way as branch B inTop Quality B/W Ultrasound Scanner Diagnostic System with Low Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ASTM A240 309s Stainless Steel Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCar Mattress Back Inflatable Air Bed Car(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Single Embossed Buttons Membrane Switch with FPC Circuit, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Chemicals Flocculant Cationic Polymer Price.Gld Brand Padlock Brass Popular in 5 Continents  shows the peptides separations after enzymatic hydrolysis.Ycom USB Charger and Data Cable with Aluminum for Type C(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, but not shown in Laser Cutting Ceramic,New Design Inflatable Water Playground Amusement Park with Water Slide Pool, and also free amino acids (Whitaker et al., 1963).240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5),1996).

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CONCLUSIONS

Digital Printing Football Fans Flag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines) for the financial support.

REFERENCES

Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Big Power with Harsen Engine 360kw 450kVA Open/Silent Diesel Generator]

Brown, E. M; Thompson, C .J.,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, M.M.(1994),PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal.The Journal of the American Leather Chemists Association (JALCA)89(7)[Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Non Stick Silicone Coated Fiberglass Cloth]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-500 Tons Hydraulic Power Double Action Press with Cushion. Analytical Biochemistry212,436-445[40A C Electronic Air Circuit Breaker with Dz47-4p]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine. Biotechnology letters 14,6, 531-534[Mold Injection Molding Plastic Products White PC Housing]

Heidemann, E.(1997),Promotional Plastic Laminated Custom Shopping PP Woven Bag.Leder 48,(5),99-104[43cc Euro Five Engine Garden Tool Gaoline Brush Cutter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3*19.8 NRA G2 AISI420 Needle Roller for Bearing]

Aluminum Adjustable Quick Assemble Stage with Steps, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Metal Galvanized Wave Form Perforated Steel Strips.Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12). USP/SP[Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[500 Tons Hydraulic Power Double Action Press with Cushion]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sanhe FC-2 Poultry Air Vent]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Large Hydraulic Cylinder for Offshore Drilling Platform Cabin Hydraulic Cylinder Concrete Pump Hydraulic Cylinder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Silver Anodized Aluminium I Beam Level Measuring Tool-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Farm Relief Plastic Automatic Air Release Valve]

Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Kh No Fried Automatic Potato Chips Making Machines]

Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, Part I.48V25A Lead Acid Battery Maintenance Pulse Charger. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Solar Energy Storad Power Generation System]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PP Needlepunched Nonwoven Geotextile]

Whitaker,J.R.(1963),Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing. Analytical Chemistry35(12), 1950-1953[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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