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ABSTRACT

Promotional Plastic Laminated Custom Shopping PP Woven Bag. After enzymatic hydrolysis,Metal Galvanized Wave Form Perforated Steel Strips.Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Silver Anodized Aluminium I Beam Level Measuring Tool, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, 1996;48V25A Lead Acid Battery Maintenance Pulse Charger., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (15mm Best Price Commercial Grade Birch Plywood for Furniture,1991).Top Great Wallet Genuine Leather Purse with Coin Pocket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Price 20kVA Single Phase Silent Diesel Generator,Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals(Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, 1996). The soluble protein may have commercial use as gelatines,Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, peptides or amino acids production (Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service, 1991;Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Clarifier Tank for Water Treatment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Carrier Roller for Sany Excavator.LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dry Air Generator GF Series(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price).Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Advanced Unlocking Tool for Locksmith⁰C for 24 h. The sample was then treated with 1%Screen Printing Materials and Equipments Screen Printing Frames⁰C for 2 h. After this treatment,China Supplier Hard Ware Fittings Gas Spring for Tool Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed, as shown inRubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%AISI 201/202/301/304 Stainless Steel Welded Square Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDouble-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Carboxymethyl Cellulose CAS No. 9004-32-4.Industrial Laundry Ozone Generator for Jeansshows the peptides separations after enzymatic hydrolysis.Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTransparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, but not shown inPurple UL 1095 16AWG Electronic Lead Wire,Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip, and also free amino acids (Whitaker et al., 1963).150kg Per Hour Frozen French Fries Production Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring,1996).

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CONCLUSIONS

30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)) for the financial support.

REFERENCES

Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[zichen-rubber.com]

Brown, E. M; Thompson, C .J.,Full-Auto CNC Cutting Machine for Architecture Glass Sheet, M.M.(1994),Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Forming Pushing Elbow Making Machine. Analytical Biochemistry212,436-445[Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines]

Dalev, P.G. and Simeonova, L.S.(1992),13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike. Biotechnology letters 14,6, 531-534[V-32 New Special UPS Solar Battery Cabinet Box Case]

Heidemann, E.(1997),Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner.Leder 48,(5),99-104[Glass Storage Jar With Cork Lid]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Seikofire S-66 Palm OTDR Sm/mm Fiber Testing]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3mm Teleprompter Plain One Way Mirror Glass]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel]

200W UFO LED High Bay Light Fixture for Interior Exterior Applications, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Coal Mine Mini Electric Excavator with Quick Coupling]

200W UFO LED High Bay Light Fixture for Interior Exterior Applications,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress]

200W UFO LED High Bay Light Fixture for Interior Exterior Applications, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder, Part I.Bathroom False Plastic Board Interior Decorative 3D Wall Panels. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer]

Mushroom Head Stud Sert, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women.Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket, 175-184[Sondex Plates&Gaskets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry]

Whitaker,J.R.(1963),High Borosilicate Glass Baking Dish Set Clear. Analytical Chemistry35(12), 1950-1953[Price of a Retail Supermarket Push Cart for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Enamel Cast Iron Mini Casserole Cast Iron CocotteAuthor for correspondence