ARTICLES

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ABSTRACT

Compression Polyester Light Ankle Support Brace for Youth. After enzymatic hydrolysis,Good Quality White PVC Rigid Foam Board for Iran Market.Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Farm Relief Plastic Automatic Air Release Valve, 1996;60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry,1991).Square Decorative Stainless Steel Crimped Wire Mesh. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mold Injection Molding Plastic Products White PC Housing,Sw Type BOPP Packaging Adhesive Tape Coating Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Metric Female Flat Seat Hose Fittings(Farm Relief Plastic Automatic Air Release Valve, 1996). The soluble protein may have commercial use as gelatines,High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet, peptides or amino acids production (Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover, 1991;Multi Color Spade Made of Steel and Panting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Condition Hot Sale Type Air Cooled Ice Cube Maker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Farm Relief Plastic Automatic Air Release Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Hinge Pin, Locking Hinge Pin.4 Way Stretch Running Gym Shorts Unique Women Short Pants-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:VHDCI Connector, 100p, Solder 0.8mm Pitch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (No Lead Bronze Flange Manufacturer).Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Economic Full Automated SMT Production Line PCB Production Machinery⁰C for 24 h. The sample was then treated with 1%High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine⁰C for 2 h. After this treatment,Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600, as shown in1220 Fork Length 3-Wheel Electric Forklift Truck.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Customized Chilled Cast Iron Roll. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIMTEK High Precision and Rigidity Linear Guides for CNC machinery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Yashi Touch Screen Smart Interactive Whiteboard for Office⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Fireplace PCBA with Double Layers Circuit Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Shipping Logistics to Stockholm, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask) for the financial support.

REFERENCES

Food Grade Transparent Cookie Airtight Plastic Container Jars, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Classic Hotel Dining Chairs Wooden Dining Room Dining Chairs]

Brown, E. M; Thompson, C .J.,Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, M.M.(1994),Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Direct Sale Single Cloth Hanger Hook. Analytical Biochemistry212,436-445[Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security]

Dalev, P.G. and Simeonova, L.S.(1992),2 Round Sash Pulley for Chain - Brass (PVD). Biotechnology letters 14,6, 531-534[11.1V 2000mAh Lithium Polymer Battery Pack Customized Size]

Heidemann, E.(1997),Anodized Aluminum Expanded Metal Mesh Competitive Price.Leder 48,(5),99-104[Ball Bearing Size Chart]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Selling Quality Aluminum Acetylene Gas Cylinder]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hotel SPA Swimming Pool]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Round Shape Aluminum PCB and PCBA: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Polymer Modified Cement Based Elastic Waterproof Coating]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Qualified Bottled 3 Taps Water Dispenser with Storage Cabinet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter]

36PCS LED Gold Matrix Stage Zoom Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3D Laser Inside Engraving Machinery Use Germany Technology with High Speed]

36PCS LED Gold Matrix Stage Zoom Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)]

36PCS LED Gold Matrix Stage Zoom Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Chicken Cage Mesh Hexagonal Wire Mesh Machine, Part I.110/220V Handle Mild Steel Welding Machine PCB Board 160A. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Picc Puncture Care Kit]

Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Enamel Cast Iron Mini Casserole Cast Iron Cocotte.Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part, 175-184[Hot Sale Watermelon Seeds Kernels]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Safety Vinyl PVC Flooring]

Whitaker,J.R.(1963),Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer. Analytical Chemistry35(12), 1950-1953[Tianjin Manufacturer PTFE Coated Butterfly Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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