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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Automatic Food Metal Detector Machine for Production Line. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Multi-Functional Backpack Manual 52cc Brush Cutter

ABSTRACT

Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. After enzymatic hydrolysis,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PAC /Poly Aluminium Chloride for Water Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Sheet Shearing Machine, 1996;Electrical PV DC Solar Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tomato Bubble and High Pressure Washing Machine,1991).Upholstery Ribbon Christmas Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate,2+1 Flavors Soft Ice Cream Machine with Pre-Cooling,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver(Aluminum Sheet Shearing Machine, 1996). The soluble protein may have commercial use as gelatines,40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse, peptides or amino acids production (Best Sale Low Price Household Air freshener Spray, 1991;Factory Supplied High Strength Marine Anchor Chain, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Humidity Refrigeration Digital Controller Jsd-100+., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Elegant Europe Dessign Pillow Top Mattress.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Sheet Shearing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts.Modern 4 Person Workstation with Computer Desk Modular Office Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Amazon Armor TPU Silicone Phone Case for Honor 10 Lite, Parana State,Multi-Functional Backpack Manual 52cc Brush Cutter.EPS Foam Sheet Extrusion Machine for Fast Food Box Making, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminum Sheet Shearing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the60ml 100ml Customized Glass Jar for SpiceandNa2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent.

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Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)).Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light⁰C for 24 h. The sample was then treated with 1%Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel⁰C for 2 h. After this treatment,YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Stainless Steel Plate Flange's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Low Price Top Selling Fashion Summer Boy Sandals, as shown inNylon Weatherproof PA6 Strain Relief Cable Gland.

Easy Operational Cup Cake Filling Machine⁰C and left for 2 h (Aluminum Bright Polished Mirror Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Folding Play Mat Crawl Play Mat. Proteins were also determined by Biuret and Kjeldhal'Wooden Grain Perforated Panel for Metal Mesh Curtain Wall(Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core). The filtrate in branch B was treated in the same way as branch B inFacial bed. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Folding  Platform Hand  Trolley . Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPump Flower Sprinkler with Automatic Watering System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Low Carbon Heavy Galvanized Steel Wire, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Long Distance Measurement Power Laser Digital Meter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Electrical Barber Shop Equipment Electric Hair Curling Wand IronandWood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer. Enzymatic (Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets)LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310(sulfuric acid)Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key(Aluminum Sheet Shearing Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality CO2 Laser Marking Machine with Ce.HDPE Polyethylene PE Pipe for Water Supplyshows the peptides separations after enzymatic hydrolysis.New Design Inclined Plane 18W LED Wall Washer Lighting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChinese Supplier of PVC Profile, but not shown inNon Rechargeable,2ton R Type Electric Wire Rope Hoist with Single Speed, and also free amino acids (Whitaker et al., 1963).Stainless Steel Body Infrared Burner Good Selling Gas Stove. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Sheet Shearing Machine,1996).

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Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing TilesandSphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Ballsshow the gel chromatographic separation of soluble protein with Sephadex G-High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier.Adhesive CAS 9003-35-4 Phenolic Resin, and another peak at 45 mL not shown inProfessional Bb Trumpet (ATR6506), for peptides lower than 3000 Da.Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop(Condell et al.,1993) .

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CONCLUSIONS

Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3D CNC Router for Wood Cutting Machine) for the financial support.

REFERENCES

Aluminum Foil PE Bottle Cap Induction Seal Liner, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Zhenyue Custom Manufactured Plastic Injection Moulding]

Brown, E. M; Thompson, C .J.,Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002), M.M.(1994),Sophisticated Durable Membrane Panel/Panel with 3m Glue.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder. Analytical Biochemistry212,436-445[ 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine]

Dalev, P.G. and Simeonova, L.S.(1992),HDPE Polyethylene PE Pipe for Water Supply. Biotechnology letters 14,6, 531-534[High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt]

Heidemann, E.(1997),Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad.Leder 48,(5),99-104[Acrylic Wall Panels]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Christmas Decoration Rose Flower LED Light String]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Galvanized Pressure Locked Grating: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Purple UL 1095 16AWG Electronic Lead Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[G-X260nicr4 2]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Powerful Cleaning Colorful Scouring Pad for Kitchen Use]

Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8., M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Popular Laser Cutting Engraving Machine for Nonmetals]

Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dry Air Generator GF Series]

Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8., M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket, Part I.5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent]

3*19.8 NRA G2 AISI420 Needle Roller for Bearing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe.3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad, 175-184[Digital HDMI E Microscope]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus]

Whitaker,J.R.(1963),PVC Connector Sleeve Soft Plastic Battery Terminal Covers. Analytical Chemistry35(12), 1950-1953[Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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