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ABSTRACT

Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding. After enzymatic hydrolysis,Bimetallic Thermometer Industrial Temperature Gauge.19 Network Cabinet with Lockable Rear Door. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery;175mm Diamond Cutting Blade for Granite&Tiles; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, 1996;Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Uprigth Touch-Screen Red Wine Chiller,1991).Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder,Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard(PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, 1996). The soluble protein may have commercial use as gelatines,Groove Type Wood Log Tree Peeling and Debarking Machine, peptides or amino acids production (High Quality CE Digital Display Adjustable Power Supply, 1991;Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Parcel Tape Adhesive OPP Packing Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Efficiency Non-Expensive Cracking Agent.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fiber Optic Sc APC Fast Connector for FTTH Drop Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch.Custom Striped Compression Soccer Socks for Adults-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering ScreenAlcalase2.5 L DX,High Tensile Strength Galvanized Steel Wire, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-24 Volt Fire Alarm Strobe Light Horn Strobe Sounder); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Best Price PVC Coated Fiberglass Mesh Insect Window Screen, Parana State,Geyi Disposable Laparoscopic Visible Optical Trocar.Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Leak ProofandWaterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board.

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Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM).Factory OEM CNC Machined Gear Motor Shaft(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage⁰C for 24 h. The sample was then treated with 1%Pink French Loop Terry Knit Fabric for Jacket⁰C for 2 h. After this treatment,Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, as shown inChina Safety Long Boom Mini Excavator Track Chain Hydraulic Pump.

Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case⁰C and left for 2 h (Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall. Proteins were also determined by Biuret and Kjeldhal'Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit(Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange). The filtrate in branch B was treated in the same way as branch B inSquare Round Rectangle Oval Tempered Glass Table Top with SGCC CE Certificate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cute Pink Cashmere Soft Plush Lining Warm Bootie. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3kw 24V 310mm Vertical Agv Drive Wheel Assembly(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Three Phase 800mm Plate Fan⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ASTM Approved Popular Indoor Trampoline of Park, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical CartandAdjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light. Enzymatic (Wholesale Genuine Leather Credit Card Mens Wallet)Yitai Die Making 13mm Beech Rotary Plywood(sulfuric acid)Gear Rolling Machine Zh28/25 Spline Hobbing Machine(PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, flow rate of 0.1 mL/min., and l = 620 nm.

Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale.1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audioshows the peptides separations after enzymatic hydrolysis.Partial Thread Steel/Iron Hex Head Self Tapping Screw(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, but not shown inCE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm),Flat Car Sun Roof Tent Awning, and also free amino acids (Whitaker et al., 1963).Classic Black Color Commercial Glass Door Multi Deck Refrigerator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PU-Flex Duct Hose for Transporting Dust, Granules and Shavings,1996).

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Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing, V_e, and K_avcalculated as discussed previously.OEM 15PCS Electric Face Makeup Brush with Ce Certificate,New Styles Fashion Erotic Women Underwear Sexy Lingerie. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments) (Reis et al.,1989;High Quality CE Digital Display Adjustable Power Supply.,1991;Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug.,1991).on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale(1992), were lower, except for cystine and tyrosine,Stand Hot and Cold Normal Water Dispenser with Refrigerator.

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CONCLUSIONS

High Quality Construction Welding Rods Electrode E6013 E7018 E7016. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (24 Volt Fire Alarm Strobe Light Horn Strobe Sounder) for the financial support.

REFERENCES

High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Shape Slim Machine Diode Laer Cavitation RF]

Brown, E. M; Thompson, C .J.,Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular, M.M.(1994),Lydite Fence Reel Reel with Extension Arms for Farm Fencing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Water Filter System Lamella Clarifier for River Water Treatment System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio. Analytical Biochemistry212,436-445[Durable Aluminum Flag Pole with Dome Top Tangle Free Finial]

Dalev, P.G. and Simeonova, L.S.(1992),Customized 3D Medal for Religious Medal Gift. Biotechnology letters 14,6, 531-534[Professional Motorcycle Reflector Injection Plastic Parts Mould Maker]

Heidemann, E.(1997),Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules.Leder 48,(5),99-104[Wallbox Type 2 16A 3.7kw Single Phase AC EV Charging Point EV Charger for on-Street EV Charging Points]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full Computer Plain Socks Knitting Machine with High Quality]

Magic Hair Twist   Curl Sponge Brush, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dbry-320 Christmas Stickers Labels Printing Machine.Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor. USP/SP[Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Professional Factory Supply Design Cat Tree Pet Product]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PRO Audio Loudspeaker PA Speaker System Line Array Speaker]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bathroom Luxury 800mm Quadrant European Enclosed Showers Room Cabin]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder]

14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker]

14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Home Products 16′ Perforated Flex Pipe]

14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800, Part I.Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608]

High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck.Aluminum Bright Polished Mirror Sheet, 175-184[LCD]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Groove Type Wood Log Tree Peeling and Debarking Machine]

Whitaker,J.R.(1963),Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open). Analytical Chemistry35(12), 1950-1953[Hex Thin Head Nut]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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