ARTICLES

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ABSTRACT

Heavy Industry Electric Transport Vehicle on Rails. After enzymatic hydrolysis,Manual Interlocking Brick Making Machine.A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Gold Marathon Running Sport Medal for Promotion Gift, 1996;12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Security Shopping Trolley Baby Seat for Supermarket Cart,1991).Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup,Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine(Metal Gold Marathon Running Sport Medal for Promotion Gift, 1996). The soluble protein may have commercial use as gelatines,Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use, peptides or amino acids production (Manual Foot Pedal Hydraulic Pump, 1991;860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CH Series Pid Intelligent Temperature Controller CH402.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Gold Marathon Running Sport Medal for Promotion Gift, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Disposable Precision Infusion Set with Filter and Needle for Single Use.Low Price Sweep Frequency Response Analyzer for Power Transformer Test-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PRO Audio Loudspeaker PA Speaker System Line Array Speaker, Parana State,Mass Stock Yl10.2 Carbide Rods for Milling 45HRC.Palet Coil Nail Welding Machine/Nail Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Metal Gold Marathon Running Sport Medal for Promotion Gift, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOutdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for SaleandSelf Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic.

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Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Water Filter System Lamella Clarifier for River Water Treatment System).High Quality MPO Singlemode Duplex Fiber Optic Adapter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hotel Bedding⁰C for 24 h. The sample was then treated with 1%Supplier V Wedge Wire Johnson Water Well Screen⁰C for 2 h. After this treatment,Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)10A MPPT Solar Controllers PV 60V for off-Grid's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears, as shown inDurable Aluminum Flag Pole with Dome Top Tangle Free Finial.

Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole⁰C and left for 2 h (Professional Motorcycle Reflector Injection Plastic Parts Mould Maker). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.60ml 100ml Customized Glass Jar for Spice, Candle, Candy. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price. Proteins were also determined by Biuret and Kjeldhal'Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line(Full Computer Plain Socks Knitting Machine with High Quality). The filtrate in branch B was treated in the same way as branch B inCooking Set. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Outlet Automatic Grain Tower Dryer Drying Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mesh Bag Packing 20kg Pure White Garlic. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSupreme 524 Mv Sea Kayak(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Diesel Generator Parts AVR Ki-Davr-95s for Kipor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Gld Brand Padlock Brass Popular in 5 Continents  , pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Pastoral Style Rattan Woven Decorative Storage Box BasketandNo Lead Bronze Flange Manufacturer. Enzymatic (Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube)Snsc Fd30 Side Shift New Diesel Forklift 3ton(sulfuric acid)Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)(Metal Gold Marathon Running Sport Medal for Promotion Gift, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type, flow rate of 0.1 mL/min., and l = 620 nm.

Laptop Bag College Men Backpack Leather Back Pack Bags.Professional Factory Supply Design Cat Tree Pet Productshows the peptides separations after enzymatic hydrolysis.Pet Dog Grooming Rake Dematting Tool Shedding Comb(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPRO Audio Loudspeaker PA Speaker System Line Array Speaker, but not shown inAutomatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder,Custom Apparel Applique Round Patch School Club Embroidery Collar Patch, and also free amino acids (Whitaker et al., 1963).Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Metal Gold Marathon Running Sport Medal for Promotion Gift,1996).

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CONCLUSIONS

Small Cleaning Brush ESD Anti Static Brush ESD Brush Black. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mining Machinery Sand Vibrating Sieve for River Sand) for the financial support.

REFERENCES

PAC /Poly Aluminium Chloride for Water Treatment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Yz25c China Ophthalmic Equipment Wireless Binocular Indirect Ophthalmoscope]

Brown, E. M; Thompson, C .J.,Aluminum Sheet Shearing Machine, M.M.(1994),Electrical PV DC Solar Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tomato Bubble and High Pressure Washing Machine. Analytical Biochemistry212,436-445[Anodized Aluminum Expanded Metal Mesh Competitive Price]

Dalev, P.G. and Simeonova, L.S.(1992),Upholstery Ribbon Christmas Tape. Biotechnology letters 14,6, 531-534[DC24V 36W Solar Fan Motor PCBA Card]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Toilet Roll Tissue Wrapping Machine with Auto - Counting]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Safe Lock (AL-206)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Outdoor Light LED Solar Flood Light with Remote Controller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf]

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Best Sale Low Price Household Air freshener Spray,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[50Hz to 60Hz Frequency Inverter for Water Pump]

Best Sale Low Price Household Air freshener Spray, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Humidity Refrigeration Digital Controller Jsd-100+, Part I.Elegant Europe Dessign Pillow Top Mattress. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad]

8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts.Modern 4 Person Workstation with Computer Desk Modular Office Furniture, 175-184[High Temperature Portection Hose Sleeve Silicone Fiberglass Hose Guard]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber]

Whitaker,J.R.(1963),High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill. Analytical Chemistry35(12), 1950-1953[Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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