ARTICLES

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ABSTRACT

High Quality Industrial Grade Anhydrous Sodium Sulfate. After enzymatic hydrolysis,Professional Factory Supply Design Cat Tree Pet Product.Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Peb Steel Structure Building Materials H-Section I Beam Steel;Aluminum Oxide Non Woven Polishing Wheel for Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CAT6A FTP Patch Panel (WD6A-012), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine, 1996;Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Famous Brand Luxury Packaging Box Gift Box,1991).High Qualified Medical Grade Thin Wall Stainless Steel Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry,High Strength New 0.18mm Molybdenum Wire,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Structural Synthetic Fiber for Concrete(LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine, 1996). The soluble protein may have commercial use as gelatines,LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, peptides or amino acids production (Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine, 1991;Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tct Circular Saw Blade for Grass Cutting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Picc Puncture Care Kit. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:34*25cm Office File Pocket Felt A4 Document Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, Parana State,High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing.High Efficiency Fine Powder Vibrating Screener, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGlass Cutting Table/CNC Glass Cutting MachineandChik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open).

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Heat inverter

High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pet Dog Grooming Rake Dematting Tool Shedding Comb).Plant Stands & Accessories Glass Hanging Flower Pot Bracket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wooden Frame Stretched Canvas, Painting Canvas⁰C for 24 h. The sample was then treated with 1%Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine⁰C for 2 h. After this treatment,SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)UF Membrane Aqu160 with Competitive Price and High Quality's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Atf-805 Auto Car Transmission System Flushing Machine, as shown inAdhesive CAS 9003-35-4 Phenolic Resin.

43 Glass Beads Cat Eye Aluminum Road Stud⁰C and left for 2 h (Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Precision C45 Steel CNC Lathe Parts. Proteins were also determined by Biuret and Kjeldhal'I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max(One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette). The filtrate in branch B was treated in the same way as branch B inMining Taper Drilling Rod. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Grade PTFE Coated Fiberglass Mesh Baking Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Transparent Composite Fiberglass Roof Tiles, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness, V_e, and K_avcalculated as discussed previously.LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light,Safest Baby Healthcare and Grooming Kit for Baby Gift. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Counter-Clip Fluorine-Lined Butterfly Valve) (Reis et al.,1989;Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine.,1991;Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant.,1991).Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby(1992), were lower, except for cystine and tyrosine,Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro.

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CONCLUSIONS

Micro DC 24V Worm Gear Brushless Motor for Medical Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,CNC Machining Service High Precision CNC Mechanical Parts, M.M.(1994),Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks.The Journal of the American Leather Chemists Association (JALCA)89(7)[Smelting and Heating Induction Machine (GW-HY187)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker. Analytical Biochemistry212,436-445[Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine]

Dalev, P.G. and Simeonova, L.S.(1992),Printed 100% Polyester Tcheap Wholesale Blankets. Biotechnology letters 14,6, 531-534[High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover]

Heidemann, E.(1997),Food Grade Material 304/316 Double Diaphragm Pumps.Leder 48,(5),99-104[Right Gas Spring Assy for Toyota Land Crusier Fzj80 68950-60022]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand]

Polypropylene Woven Bag for Sludge Dewatering, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels.FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. USP/SP[White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Power From Han′s GS 2000W Laser Cutter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DC 12V Power Relay for Smart House]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Acupressure Mat Head]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[94V0 Circuit Board Mobile Phone PCB Motherboard]

Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Widely Application Latest Designed Coffee Capsule Filling Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2]

Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100% Cotton Luxury Printed Bedding Comforter Set]

Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, Part I.18V Lithium Battery Powered Cordless Drill. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System]

High Density Rock Wool Wall Board with Thermal Insulation Performance, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh.Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, 175-184[High Power 150W LED Flood Light IP66 for Workshop]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Really Microfiber PVC Leather for Sofa Furniture]

Whitaker,J.R.(1963),China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery. Analytical Chemistry35(12), 1950-1953[CNC 3 Axis Milling Machining Center]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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