ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-SHXJ1500 CNC Diamond Wire Stone Cutting Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag

ABSTRACT

2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. After enzymatic hydrolysis,Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball.Yyh Series One Dimension Tumbling Mixer Mixing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ODU 14 Pin Industrial Circular Connector;Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Classical New Coming Plastic Female Sunglasses, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Double Sided Rigid PCB Circuit Board Manufacturer, 1996;Robot of World Robotics Competition., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Rope/Cable/Wire Braiding Machine with Factory Price,1991).Aluminum PCB Circuit Board SMD PCBA LED PCB. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159),Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Supplier Steel Machine Bellows Cover Telescopic Shield Cover(OEM Double Sided Rigid PCB Circuit Board Manufacturer, 1996). The soluble protein may have commercial use as gelatines,Concrete Cure Spray Concrete Curing Agent, peptides or amino acids production (Automatic Chewing Dog Food Snack Machine, 1991;White Ceramic Shot Glass for Sublimation Printing - 1.5oz, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Promotional Gifts Custom Printed Silicone Wristband/Bracelet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Double Sided Rigid PCB Circuit Board Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Farrowing Crate Pig Floor Cast Iron Slat Floor.Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Aluminum Heat Sink for Electronics Computer Electric(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns, Parana State,Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (OEM Double Sided Rigid PCB Circuit Board Manufacturer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAs568 Standard Brown FKM Rubber O Ring for SealingandHigh Speed Roll Plastic T-Shirt Bag Making Machine.

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Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag).Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block⁰C for 24 h. The sample was then treated with 1%High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card⁰C for 2 h. After this treatment,T90 High Power Latching Relay Mini Power Relay. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430, as shown inHotel Steel Digital Room Safes with Large LED Display.

Polyglycolic Acid Absorable Suture⁰C and left for 2 h (Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.No. 1062 20PCS Tools. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant. Proteins were also determined by Biuret and Kjeldhal'Aluminum Mould Shoe Mould Making CNC Milling Router Machine(Custom Logo Cotton Terry Oversize Pullover Men Hoodie). The filtrate in branch B was treated in the same way as branch B inBest Quality Makeup Brush. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Pressure Fitting Quick Coupling Hydraulic Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRefrigeration Digital Temperature Controller Stc-100A(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Black Color Hexagon Bolt with High Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042).Custom Aluminum Cover Frame Half Aluminum Cover Profileshows the peptides separations after enzymatic hydrolysis.Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLow Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA, but not shown inCarbon Graphite Crucible,Single Stage High Speed Fruit and Vegetable Pulping Machine, and also free amino acids (Whitaker et al., 1963).China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Double Sided Rigid PCB Circuit Board Manufacturer,1996).

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Prefab Expandable Luxury Living Container House Prefabricated House, V_e, and K_avcalculated as discussed previously.Movable Partition Walls for Room Division, Hotel and Shopping Mall,Carboxy Terminal Collagen Crosslinks Elisa Kits. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM) (Reis et al.,1989;Automatic Chewing Dog Food Snack Machine.,1991;Robot of World Robotics Competition.,1991).Various Colors Nylon and Stainless Steel Kitchen Tools(1992), were lower, except for cystine and tyrosine,355nm UV Laser Marking Equipment for Crystal Materials.

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CONCLUSIONS

Metal Separating Gold Brass Zippers for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Steam Eye Mask Heating One Eye Mask for Sleeping) for the financial support.

REFERENCES

Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Trifold Makeup Mirror]

Brown, E. M; Thompson, C .J.,OEM Refrigeration Digital Thermometer, M.M.(1994),BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L).The Journal of the American Leather Chemists Association (JALCA)89(7)[Low Pressure PU Filter Gasket Sandal Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Portable Telescopic Straw Metal Drinking Straw with Brush Case]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making. Analytical Biochemistry212,436-445[High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter]

Dalev, P.G. and Simeonova, L.S.(1992),79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar. Biotechnology letters 14,6, 531-534[High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain]

Heidemann, E.(1997),Two Roll Rubber & Plastic Mixing Mills Tire Machine.Leder 48,(5),99-104[Stainless Steel Mesh Sieve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Galvanized Wave Form Perforated Steel Strips]

Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes.Hot Sale 25kw Grid Tied Home Solar Panel System Kit. USP/SP[Best Price PVC Coated Fiberglass Mesh Insect Window Screen]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mini Car Portable Fridge 10L Mini Fridge: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ 3FT 3000lm IP66 Approved]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100% Sodium Cross-Linked Gracederm Hyaluronate Acid Gel Deep Filler 1ml/2ml]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Swim Shorts Grey Mens Board Shorts with Mesh Liner]

Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mini Small Garden Excavator Digging Work-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800]

Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube]

Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver, Part I.Offshore Drilling Platform Air Winch for Marine Oilfields. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Transparent Composite Fiberglass Roof Tiles]

Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heavy Industry Electric Transport Vehicle on Rails.Manual Interlocking Brick Making Machine, 175-184[Multi Function Oscillating Carbide Tooth Saw Blade]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine]

Whitaker,J.R.(1963),A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve. Analytical Chemistry35(12), 1950-1953[ with Handle Vegetable Oil Nonstick]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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