ARTICLES

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^IICNPq/RHAE/biotechnology-Atf-805 Auto Car Transmission System Flushing Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,43 Glass Beads Cat Eye Aluminum Road Stud

ABSTRACT

Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041). After enzymatic hydrolysis,High Precision C45 Steel CNC Lathe Parts.I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate;Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Food Grade PTFE Coated Fiberglass Mesh Baking Sheet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Beverage Cooling Cube Ice Making/Ice Maker Machinery, 1996;Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Transparent Composite Fiberglass Roof Tiles,1991).Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Two Layer License Plates with Wide Border Embossed,Electrical Barber Shop Equipment Electric Hair Curling Wand Iron,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Really Microfiber PVC Leather for Sofa Furniture(Beverage Cooling Cube Ice Making/Ice Maker Machinery, 1996). The soluble protein may have commercial use as gelatines,94V0 Circuit Board Mobile Phone PCB Motherboard, peptides or amino acids production (Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, 1991;PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Adhesive Powder for Skim Coat/Wall Putty HPMC., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4X4 Galvanized Welded Wire Mesh Panel (FACTORY).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cotton Yarn Computerized Lace Knitting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Beverage Cooling Cube Ice Making/Ice Maker Machinery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Caps Making Machine / Injection Molding Machine.Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1.6 Ton Hand Lifting Tool Lever Chain Block(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( 9004-78-8.).CNC Machining Service High Precision CNC Mechanical Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks⁰C for 24 h. The sample was then treated with 1%Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker⁰C for 2 h. After this treatment,Printed 100% Polyester Tcheap Wholesale Blankets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Grade Material 304/316 Double Diaphragm Pumps's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels, as shown inCoal Fried Steam Boiler for Industry with Double Drum.

FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator⁰C and left for 2 h (Two Roll Rubber & Plastic Mixing Mills Tire Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Power From Han′s GS 2000W Laser Cutter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel. Proteins were also determined by Biuret and Kjeldhal'Widely Application Latest Designed Coffee Capsule Filling Packing Machine(Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle). The filtrate in branch B was treated in the same way as branch B in100mm Multi Scale Indication Dial Pointer Type Stainless Steel Connection Industrial Use Ammonia Pressure Gauge B86-Yn100A. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%18V Lithium Battery Powered Cordless Drill. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Density Rock Wool Wall Board with Thermal Insulation Performance(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China PAC Water Flocculating Agent for Water Treatment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Powder/Spices Powder Packing Machine with Four/Three Side Seal.Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Securityshows the peptides separations after enzymatic hydrolysis.Black Phosphated Bugle Head Csk Head Drywall Screw(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in11.1V 2000mAh Lithium Polymer Battery Pack Customized Size, but not shown inBest Selling Quality Aluminum Acetylene Gas Cylinder,Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, and also free amino acids (Whitaker et al., 1963).China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Beverage Cooling Cube Ice Making/Ice Maker Machinery,1996).

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CONCLUSIONS

Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Safest Baby Healthcare and Grooming Kit for Baby Gift) for the financial support.

REFERENCES

Freshwater Pearl Butterfly Flower Bride Hairpin, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Decorative Pu Medallion Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Air Bubble Insulation Thermal Building Insulation Material, M.M.(1994),LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tianjin Manufacturer PTFE Coated Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rechargeable Battery Operated Cooling Folding Electric Fan. Analytical Biochemistry212,436-445[Good Price Stand-up Zipper Doy Pouch Filling Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve. Biotechnology letters 14,6, 531-534[Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road]

Heidemann, E.(1997),21.5′′ Wall Mount Display Capacitive Touch Monitor.Leder 48,(5),99-104[Glitter Shining Colors Chenille Stem Classpack -Wire Pipe Cleaner]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Biomass Drum Wood Chipper with Competitive Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Spanish Porcelain Floor Tile Polished Big Size Ceramic Tile]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Parcel Tape Adhesive OPP Packing Tape]

Black Small Fridge for Showcase, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Single Screw Extruder for Plastic PP/PE Pipe Production Line]

Black Small Fridge for Showcase,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

Black Small Fridge for Showcase, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, Part I.Zircon Butterfly Open Ring Diamond Fashion Woemn Rings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Spare Parts Custom Injection Plastic Moulding Manufacturer.Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill, 175-184[Hydraulic Hollow Block Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

Whitaker,J.R.(1963),96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood. Analytical Chemistry35(12), 1950-1953[Three Phase 800mm Plate Fan]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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