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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.AISI 201/202/301/304 Stainless Steel Welded Square Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce, 1996;Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger,1991).PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road,High Speed Copper Wire Braiding Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Safe Durable Construction Scaffolding Plank for Building(Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce, 1996). The soluble protein may have commercial use as gelatines,Different Size of Ring NdFeB Magnet, peptides or amino acids production (Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine, 1991;High Quality Welding Structure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Carboxymethyl Cellulose CAS No. 9004-32-4., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰150kg Per Hour Frozen French Fries Production Line.Automatic Professional LED Display Ceramic Hair Curling Iron-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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As568 Standard Brown FKM Rubber O Ring for Sealing⁰C and left for 2 h (CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card. Proteins were also determined by Biuret and Kjeldhal'200W UFO LED High Bay Light Fixture for Interior Exterior Applications(Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings). The filtrate in branch B was treated in the same way as branch B inm.qj-ec.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBathroom False Plastic Board Interior Decorative 3D Wall Panels(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Borosilicate Glass Baking Dish Set Clear, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Concrete Spare Part Electrical System Central Distribution Box.Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDFshows the peptides separations after enzymatic hydrolysis.Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared infrom China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, but not shown inHigh Speed Cable Wire Winding Braiding Machine,50Hz to 60Hz Frequency Inverter for Water Pump, and also free amino acids (Whitaker et al., 1963).Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce,1996).

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PVC Cabinet Construction Board, White 18mm PVC Foam Board, V_e, and K_avcalculated as discussed previously.2019 New Portable Mini Dental X-ray Machine,Ss Reduced Port 2000wog 1PC Screwed Ball Valve. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness) (Reis et al.,1989;Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine.,1991;Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss.,1991).China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous(1992), were lower, except for cystine and tyrosine,China Prefab Steel Building for Garment Factory.

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CONCLUSIONS

Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low) for the financial support.

REFERENCES

Cable Tray with ′t′ Slots, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Alexa Smart WiFi LED Strip 5m 300 LEDs Smart LED Light Compatible with Tuya Smart APP/Siri]

Brown, E. M; Thompson, C .J.,10 Gauge Galvanized Welded Wire Mesh for Construction Using, M.M.(1994),High Quality Factory Inline Hockey Skate Puck.The Journal of the American Leather Chemists Association (JALCA)89(7)[PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminum Mould Shoe Mould Making CNC Milling Router Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector. Analytical Biochemistry212,436-445[10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light]

Dalev, P.G. and Simeonova, L.S.(1992),201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet. Biotechnology letters 14,6, 531-534[Low Energy Needed Compact Flour Mill Plant for Milling Wheat]

Heidemann, E.(1997),Disposable PS Foam Fast Food Box Making Machine.Leder 48,(5),99-104[Horizontal Cnc Milling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sower Vacuum Emulsifying Machine for Cosmetic]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Quality Bending Metal Mould with All Kinds Metal Materials]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Foska Good Quality Plastic Stapler Set with Staples: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medical Temperature Sensor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System]

Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hotel SPA Swimming Pool, Sport Room, Bathroom Towel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding]

Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Powerful Cleaning Colorful Scouring Pad for Kitchen Use, Part I.500t Plywood Hot Press Woodworking Machine (12-25 layers). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion Shoulder Backpack Handbag School Bag]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope]

Whitaker,J.R.(1963),5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine. Analytical Chemistry35(12), 1950-1953[Security Shopping Trolley Baby Seat for Supermarket Cart]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Silver Anodized Aluminium I Beam Level Measuring ToolAuthor for correspondence