ARTICLES

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ABSTRACT

Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder. After enzymatic hydrolysis,Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag.VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pet Anti-Static Needled Mat, 1996;Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Sheet Metal Fabrication for Aluminum Parts,1991).Hwf Tsp Core Bit for Geotechnical Drilling. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Price of Automatic Sludge Dewatering Machine,Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Worm Gear Operation Wafer Lined F3 Butterfly Valve(Pet Anti-Static Needled Mat, 1996). The soluble protein may have commercial use as gelatines,Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad, peptides or amino acids production (Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag, 1991;Positive Drive Turn Belt for Spiral Freezing Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chinese Herb Medicine Angelica Root Extract., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.S Series China Generator Helical-Worm Crushers Reduction Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pet Anti-Static Needled Mat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Original Testo 174t Mini Temperature Data Logger No. 0572 1560.Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Solar Panel Solar Cell Photovoltaic 300W Hot Sell, Parana State,Sinicline Custom Brand with Plastic Pouch Hangtag for Hats.Vacuum Coating Machine Water Chiller (CW-6300AN), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pet Anti-Static Needled Mat, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food StorageandPromotional Plastic Laminated Custom Shopping PP Woven Bag.

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Planter Moving Round Planter Saucer Casters Plant Trolley (M)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Granules and Shavings).1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)⁰C for 24 h. The sample was then treated with 1%Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts⁰C for 2 h. After this treatment,CNC Machining Non-Standard Hardware Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Galvanized Solar System Mounting Bracket Connector, as shown inDetergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate.

Digital Programmable Ntc Temperature Controller⁰C and left for 2 h (2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Water Meter Spare Parts Rubber Rings for Sealing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel HK Series Needle Roller Bearing HK0408. Proteins were also determined by Biuret and Kjeldhal'Road Construction Fabric Non-Woven Geotextile 200GSM(Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires). The filtrate in branch B was treated in the same way as branch B inNice Design Curing Oven Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Polymer Modified Cement Based Elastic Waterproof Coating.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHeat-Sealed Laminated Bag with Hexagonal Bottom Gusset(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl, pH 3.25., mobile phase B: buffer solution of 0.01 mol/FTTH Fiber Optic Fusion Splicing Toolkit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card.Trunk Organizer Foldable Water Proofshows the peptides separations after enzymatic hydrolysis.Residential Reverse Osmosis 0.0001um Water Filter Water Filtration(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, but not shown inDay & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular,Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle, and also free amino acids (Whitaker et al., 1963).China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pet Anti-Static Needled Mat,1996).

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Powder/Spices Powder Packing Machine with Four/Three Side SealandOEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Partsshow the gel chromatographic separation of soluble protein with Sephadex G-Folding Round Picnic Caravan Table RV Picnic Table Camping Table.Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, and another peak at 45 mL not shown inCopy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, for peptides lower than 3000 Da.3HP Industrial Cooling System Water Cooled Chiller(Condell et al.,1993) .

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CONCLUSIONS

32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dia. 5.5mm Bonding ND: YAG Laser Rod Lens) for the financial support.

REFERENCES

Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Replacement Pfc8314-50-Yv Filter Purifiers]

Brown, E. M; Thompson, C .J.,Corrugated Board Production Line, M.M.(1994),Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive.The Journal of the American Leather Chemists Association (JALCA)89(7)[Various Colors Nylon and Stainless Steel Kitchen Tools]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Car Air Conditional Compressor Magetic Clutch 5pk. Analytical Biochemistry212,436-445[Factory Outlet Automatic Grain Tower Dryer Drying Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Engieered Veneer Faced Commericial Plywood. Biotechnology letters 14,6, 531-534[High Quality CE Digital Display Adjustable Power Supply]

Heidemann, E.(1997),Hex Thin Head Nut.Leder 48,(5),99-104[Bathroom Sinks]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430]

Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2.Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap. USP/SP[Factory Sell P7.8 Transparent LED Display Screen]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White Cotton Satin Hotel Duvet Cover (DPFB8055)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Spare Parts for Caterpillar Construction Machinery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bubble Film Lamination Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cold Rolled Stainless Steel Strip /Coils 410/430/409]

Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),50 Grams Ozone Producing Device for Makeup Toner Producing Plant-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine]

Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home]

Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flat Pack Temperary Container House 20FT 40FT, Part I.Sweat Suit for Women Three Piece Tracksuit. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Double Layer Filter USD in POY Machines]

Cold Rolled Stainless Steel Strip /Coils 410/430/409, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FRP Water Tank GRP Fishing Tanks From Factory Supply.Decorative House Stone Coated Metal Roof Tile, 175-184[www.tysonleather.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Stainless Steel Expanded Metal Mesh]

Whitaker,J.R.(1963),Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig. Analytical Chemistry35(12), 1950-1953[Multi-Functional Backpack Manual 52cc Brush Cutter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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