ARTICLES

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ABSTRACT

LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. After enzymatic hydrolysis,External Retaining Ring for Shaft, DIN471, D1400.Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Rubber Product Seal O-Ring /NBR70 O Ring, 1996;Fully Automatic Dust Face Mask Making Machine with Ultrasonic., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3D Laser Inside Engraving Machinery Use Germany Technology with High Speed,1991).Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle,Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints(Custom Rubber Product Seal O-Ring /NBR70 O Ring, 1996). The soluble protein may have commercial use as gelatines,DIN 7337 Aluminium Core Pulling Stainless Steel Rivet, peptides or amino acids production (Professional Bb Trumpet (ATR6506), 1991;Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3.5ton Chinese Engine Gasoline Forklift (FG35T)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Rubber Product Seal O-Ring /NBR70 O Ring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterproof Covering Material Interior Wall Composite Panel.Tall/Large Cube Glass Terrarium Planter Gift Card Holder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Venlo Type Flower Multi Span Commercial Glass Greenhouses Used(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Deep Hole Cylinder Boring Machine, Parana State,Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch.Good Prize Solar PV Mounting Panel System for Ground, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Rubber Product Seal O-Ring /NBR70 O Ring, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the110/220V Handle Mild Steel Welding Machine PCB Board 160AandHardware CNC Machinery Milling Part.

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Electric Heavy Duty Rail Guided Vehicle for Transportation(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT).Sower Vacuum Emulsifying Machine for Cosmetic(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ASTM A240 309s Stainless Steel Sheet⁰C for 24 h. The sample was then treated with 1%Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home⁰C for 2 h. After this treatment,Car Mattress Back Inflatable Air Bed Car. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine, as shown inLaundry Used Gas Water Heater with CE/Asme Certificate.

Single Embossed Buttons Membrane Switch with FPC Circuit⁰C and left for 2 h (Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Popular Laser Cutting Engraving Machine for Nonmetals. Proteins were also determined by Biuret and Kjeldhal'Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit(2.0mm Yellow Markers for Fiber Optic Multi Patch Cord). The filtrate in branch B was treated in the same way as branch B inDisposable Medical Products Repeated Use Type Needle Puncture Needle With Side Air. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Large Slewing Rings Bearings for Wind Turbine Ring on Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ycom USB Charger and Data Cable with Aluminum for Type C⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Design Inflatable Water Playground Amusement Park with Water Slide Pool, pH 3.25., mobile phase B: buffer solution of 0.01 mol/240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stand Hot and Cold Normal Water Dispenser with RefrigeratorandLight Weight Polyester Eye Mask for Travel Airplane Hotel. Enzymatic (6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8))2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable(sulfuric acid)Bamboo Tablet Stand Laptop Rack Bt-2212(Custom Rubber Product Seal O-Ring /NBR70 O Ring, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth) for the financial support.

REFERENCES

Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electric Hard Seal Fixed Ball Valve]

Brown, E. M; Thompson, C .J.,Silver Anodized Aluminium I Beam Level Measuring Tool, M.M.(1994),Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-48V25A Lead Acid Battery Maintenance Pulse Charger. Analytical Biochemistry212,436-445[Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange]

Dalev, P.G. and Simeonova, L.S.(1992),15mm Best Price Commercial Grade Birch Plywood for Furniture. Biotechnology letters 14,6, 531-534[Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart]

Heidemann, E.(1997),Top Great Wallet Genuine Leather Purse with Coin Pocket.Leder 48,(5),99-104[aihdcam]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Low-Price Medical Laughing N2o Gas Cylinders (25L/40L)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden]

Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door]

Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mass Stock Yl10.2 Carbide Rods for Milling 45HRC]

Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer, Part I.Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Size 000 Clear Pullulan Vegetarian Capsules]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments]

Whitaker,J.R.(1963),LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes. Analytical Chemistry35(12), 1950-1953[Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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