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ABSTRACT

13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder. After enzymatic hydrolysis,Bathroom False Plastic Board Interior Decorative 3D Wall Panels.Mushroom Head Stud Sert. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Borosilicate Glass Baking Dish Set Clear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, 1996;Embossing Roller for Tissue Paper., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel,1991).Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Colorful  Reflective Sticker Reflective Tape,Concrete Spare Part Electrical System Central Distribution Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress(G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, 1996). The soluble protein may have commercial use as gelatines,50Hz to 60Hz Frequency Inverter for Water Pump, peptides or amino acids production (Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330), 1991;High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVC Cabinet Construction Board, White 18mm PVC Foam Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 New Portable Mini Dental X-ray Machine.Ss Reduced Port 2000wog 1PC Screwed Ball Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Factory Inline Hockey Skate Puck, Parana State,China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2).0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down LightandGalvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe.

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201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)).Disposable PS Foam Fast Food Box Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)⁰C for 24 h. The sample was then treated with 1%Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP⁰C for 2 h. After this treatment,Foska Good Quality Plastic Stapler Set with Staples. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hotel SPA Swimming Pool, Sport Room, Bathroom Towel, as shown inFoshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration.

Powerful Cleaning Colorful Scouring Pad for Kitchen Use⁰C and left for 2 h (43 Glass Beads Cat Eye Aluminum Road Stud). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.500t Plywood Hot Press Woodworking Machine (12-25 layers). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10ml Round Clear Nail Polish Bottle with Brush Cap. Proteins were also determined by Biuret and Kjeldhal'Full Automatic 4000b/h 500ml Plastic Jar Making Machine(Water Cooled Low Temperature Screw Chiller for Industrial Workshop). The filtrate in branch B was treated in the same way as branch B inhigh frequency transformer suppliers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPCB18h 8p8c Modular Plug with Shield Head RJ45 Connector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Laundry Used Gas Water Heater with CE/Asme Certificate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/15W Aquarium Submersible UV Lamp Durable Germicidal Lamp, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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What′s Kind of Machine for Making Organic Fertilizer Granules.Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bagshows the peptides separations after enzymatic hydrolysis.Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGlass Lifting Machine with Sucker for Glass Movements, but not shown inFlat Car Sun Roof Tent Awning,Home and Hospital Electric Patient Lift, and also free amino acids (Whitaker et al., 1963).Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen,1996).

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CONCLUSIONS

Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cable Tray with ′t′ Slots) for the financial support.

REFERENCES

RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable]

Brown, E. M; Thompson, C .J.,48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket, M.M.(1994),Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52. Analytical Biochemistry212,436-445[6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder]

Dalev, P.G. and Simeonova, L.S.(1992),China Supplier Granular Coal Based Activated Carbon Filter Meida. Biotechnology letters 14,6, 531-534[Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming]

Heidemann, E.(1997),Glass Cutting Table/CNC Glass Cutting Machine.Leder 48,(5),99-104[Cabinet Shoe Organizer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dn32 (11/4) BSPT Thread Air Actuated Piston Valves]

Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),12 Bottles Portable Home Ues Thermoelectric Wine Chiller.Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter. USP/SP[32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chicken Cage Mesh Hexagonal Wire Mesh Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Car Auto Seat Trunk Cover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Roller Wedge Screen Cylinder]

Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Freshwater Pearl Butterfly Flower Bride Hairpin]

Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women]

Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle, Part I.Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Elbow Forming Machine Gorelocker HVAC Duct Making Machine]

Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve.Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron, 175-184[X-ray Film/Medical Film/X-ray/Medical/Film/Radiology Film/Analogue Film/Wet Film/Conventional Film/Medical Consumables/Green Sensitive/Blue Sensitive/35X35cm]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver]

Whitaker,J.R.(1963),Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch. Analytical Chemistry35(12), 1950-1953[Customize Milky Extrusion PC Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining ChairAuthor for correspondence