ARTICLES

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ABSTRACT

Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length. After enzymatic hydrolysis,Non Stick Silicone Coated Fiberglass Cloth.Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, 1996;White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale 100% Polyester Cartoon Kids Sleepwear,1991).Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater,Laparoscopy Clip Applicator for Abdominal Surgery Uses,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Yield Tree Stump Crusher Machine(3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, 1996). The soluble protein may have commercial use as gelatines,Diaphragm Valve for Bag Filter Since 1992, peptides or amino acids production (4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, 1991;Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manufacturer Plastic Injection Mould Molding Nylon PP Injection., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Forging Cast Metal Sand Die Casting.ADC12 Aluminum Die Casting Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PVC Fabric Rapid Roller Shutter for Clearn Room, Parana State,Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag.Fiberglass Animal Poultry Insulation Grain Tower, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSs Reduced Port 2000wog 1PC Screwed Ball ValveandResidential Reverse Osmosis 0.0001um Water Filter Water Filtration.

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Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine).Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale 375ml Dark Green Glass Ice Wine Bottles⁰C for 24 h. The sample was then treated with 1%Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle⁰C for 2 h. After this treatment,Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cog Graphic FPC LCD Display Module's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Power Fitting- Hot DIP Galvanizing Bolt with Nut, as shown inBelt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low.

Slewing Gear for Tower Crane Slewing Winch Reducer⁰C and left for 2 h (Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side. Proteins were also determined by Biuret and Kjeldhal'High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes(Electric Fireplace PCBA with Double Layers Circuit Board). The filtrate in branch B was treated in the same way as branch B inCircuit Boards. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTelescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Single Screw Extruder for Plastic PP/PE Pipe Production Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic Injection Moulding Machine Supplier / Factory /ManufacturerandGold Star Square Glass Candle Holder for Wedding Decoration. Enzymatic (11kg 24L Low Pressure LPG Gas Cylinder)Commercial Use Click Lock Standard Spc Vinyl Plank Flooring(sulfuric acid)Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop(3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer) for the financial support.

REFERENCES

Disposable Anesthesia Breathing Circuit Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[H331 A331 Carbon or Alloy Grade 43 Clevis Slip Hoist Hooks]

Brown, E. M; Thompson, C .J.,High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type, M.M.(1994),Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Granules and Shavings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126. Analytical Biochemistry212,436-445[Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate]

Dalev, P.G. and Simeonova, L.S.(1992),Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks. Biotechnology letters 14,6, 531-534[2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus]

Heidemann, E.(1997),Counter-Clip Fluorine-Lined Butterfly Valve.Leder 48,(5),99-104[Aluminium Coil Manufacturers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires]

Outdoor Garden Save Energy with Solar Flame Wall Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),DC Brushless Motor for CNC Machine with ISO9001.Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck. USP/SP[Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Trunk Organizer Foldable Water Proof]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Road Safety Warning Barricade LED Solar Powered Blinking Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Sale Christmas Crystal Ball Music Box for Promotion]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular]

Manufacturer Customized Plastic Injection Molding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank]

Manufacturer Customized Plastic Injection Molding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Powder/Spices Powder Packing Machine with Four/Three Side Seal]

Manufacturer Customized Plastic Injection Molding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve, Part I.Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts]

60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Solar Energy Storad Power Generation System.110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K), 175-184[Three-Head Automatic Sealing Strip Filling Machine for Tin Can Making]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine]

Whitaker,J.R.(1963),Custom Aluminum Cover Frame Half Aluminum Cover Profile. Analytical Chemistry35(12), 1950-1953[Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Air Bubble Insulation Thermal Building Insulation MaterialAuthor for correspondence