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ABSTRACT

Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp. After enzymatic hydrolysis,T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm).High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder;Mechanical Seal 101 Type for Pump and Auto; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass, 1996;China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus,1991).Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube,3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Low Pressure PU Filter Gasket Sandal Making Machine(Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass, 1996). The soluble protein may have commercial use as gelatines,Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, peptides or amino acids production (Flour Making Machine Wheat Maize Flour Mill Machinery, 1991;Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metal Material and Sports Theme Custom Metal Medal.Portable Installation and Ce Certification 20g/Hr OEM Air Purifier-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Printed 100% Polyester Tcheap Wholesale Blankets).Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products⁰C for 24 h. The sample was then treated with 1%Made in China Lqa Strap Banding Tool⁰C for 2 h. After this treatment,New R&D Wire Cable Feeding Winding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Residence Use 220V 50Hz 12kw Portable silent Diesel Generator, as shown in150kg Per Hour Frozen French Fries Production Line.

Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain⁰C and left for 2 h (Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header. Proteins were also determined by Biuret and Kjeldhal'Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly(Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)). The filtrate in branch B was treated in the same way as branch B inAluminum Parts Cnc Machining Services. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Galvanized Roller Shutter Window Torsion Springs.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarbon Graphite Crucible(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Magic Hair Twist   Curl Sponge Brush, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Borosilicate Glass Baking Dish Set ClearandClearing Clogged Effectively Pipe Drain Cleaning Machine (A75). Enzymatic (Geyi Disposable Laparoscopic Visible Optical Trocar)Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding(sulfuric acid)Bimetallic Thermometer Industrial Temperature Gauge(Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%19 Network Cabinet with Lockable Rear Door, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%19 Network Cabinet with Lockable Rear Door, flow rate of 0.1 mL/min., and l = 620 nm.

Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery.Plant Stands & Accessories Glass Hanging Flower Pot Bracketshows the peptides separations after enzymatic hydrolysis.175mm Diamond Cutting Blade for Granite&Tiles(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectric Magic Hot Hair Styling Curling Iron with Brushes, but not shown in21.5′′ Wall Mount Display Capacitive Touch Monitor,Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, and also free amino acids (Whitaker et al., 1963).PU-Flex Duct Hose for Transporting Dust, Granules and Shavings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass,1996).

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CONCLUSIONS

Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)) for the financial support.

REFERENCES

Parcel Tape Adhesive OPP Packing Tape, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Multifunctional Shr Opt IPL/IPL Laser/IPL Hair Removal Tattoo Removal IPL Shr Opt Laser Permanent Hair Removal Beauty Salon Equipment Beauty Instrument]

Brown, E. M; Thompson, C .J.,High Efficiency Non-Expensive Cracking Agent, M.M.(1994),Fiber Optic Sc APC Fast Connector for FTTH Drop Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality CO2 Laser Marking Machine with Ce]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel HK Series Needle Roller Bearing HK0408]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch. Analytical Biochemistry212,436-445[Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Striped Compression Soccer Socks for Adults. Biotechnology letters 14,6, 531-534[Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting]

Heidemann, E.(1997),Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738).Leder 48,(5),99-104[Activated Alumina for Petrochemical Catalyst Carrier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glass Cutting Table/CNC Glass Cutting Machine]

Steel Forged Automobile Spare Parts with Customized Drawing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen.High Tensile Strength Galvanized Steel Wire. USP/SP[Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Laptop Bag College Men Backpack Leather Back Pack Bags]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-24 Volt Fire Alarm Strobe Light Horn Strobe Sounder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pet Dog Grooming Rake Dematting Tool Shedding Comb]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Teslacigs Wye 85W Vape Mod with Innovative Taste]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Adhesive CAS 9003-35-4 Phenolic Resin]

from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Price PVC Coated Fiberglass Mesh Insect Window Screen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad]

from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette]

from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, Part I.Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse]

Factory OEM CNC Machined Gear Motor Shaft, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage.Pink French Loop Terry Knit Fabric for Jacket, 175-184[Nozzles]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Box Sealing Packing Taping Strapping Machine]

Whitaker,J.R.(1963),Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb. Analytical Chemistry35(12), 1950-1953[4-14 Manual Staple Gun with Staples for Hand Tools]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Customized Molded Best Silicone Glass Water Drinking Bottle SleeveAuthor for correspondence