ARTICLES

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ABSTRACT

Large Slewing Rings Bearings for Wind Turbine Ring on Sale. After enzymatic hydrolysis,Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container.Chemicals Flocculant Cationic Polymer Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Light Weight Polyester Eye Mask for Travel Airplane Hotel, 1996;6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable,1991).Bamboo Tablet Stand Laptop Rack Bt-2212. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book,Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Personal Care Glutathione Skin Whitening Tablets(Light Weight Polyester Eye Mask for Travel Airplane Hotel, 1996). The soluble protein may have commercial use as gelatines,Customized Over Size Boys Hoodie with Embroidered, peptides or amino acids production (Digital Printing Football Fans Flag, 1991;Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-500 Tons Hydraulic Power Double Action Press with Cushion. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Light Weight Polyester Eye Mask for Travel Airplane Hotel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine.Promotional Plastic Laminated Custom Shopping PP Woven Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metal Galvanized Wave Form Perforated Steel Strips(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, Parana State,Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions.48V25A Lead Acid Battery Maintenance Pulse Charger, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Light Weight Polyester Eye Mask for Travel Airplane Hotel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuseand Box Sealing Packing Taping Strapping Machine.

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15mm Best Price Commercial Grade Birch Plywood for Furniture(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve).Top Great Wallet Genuine Leather Purse with Coin Pocket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Price 20kVA Single Phase Silent Diesel Generator⁰C for 24 h. The sample was then treated with 1%Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing⁰C for 2 h. After this treatment,Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service, as shown inAluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine.

Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer⁰C and left for 2 h (Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Clarifier Tank for Water Treatment(Scouring Pads Making Machine for Brush King). The filtrate in branch B was treated in the same way as branch B inIP44 New Recessed Down Light From Venezina 6W/10W/15W. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Carrier Roller for Sany Excavator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002), pH 3.25., mobile phase B: buffer solution of 0.01 mol/2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Screen Printing Materials and Equipments Screen Printing Frames.Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bagshows the peptides separations after enzymatic hydrolysis.China Supplier Hard Ware Fittings Gas Spring for Tool Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCounter-Clip Fluorine-Lined Butterfly Valve, but not shown inGamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge,Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, and also free amino acids (Whitaker et al., 1963).Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Light Weight Polyester Eye Mask for Travel Airplane Hotel,1996).

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CONCLUSIONS

Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale) for the financial support.

REFERENCES

PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Factory Polymers PAM Flocculant]

Brown, E. M; Thompson, C .J.,Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, M.M.(1994),High Speed Copper Wire Braiding Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[DC 12V Power Relay for Smart House]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[94V0 Circuit Board Mobile Phone PCB Motherboard]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Safe Durable Construction Scaffolding Plank for Building. Analytical Biochemistry212,436-445[Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2]

Dalev, P.G. and Simeonova, L.S.(1992),Different Size of Ring NdFeB Magnet. Biotechnology letters 14,6, 531-534[100% Cotton Luxury Printed Bedding Comforter Set]

Heidemann, E.(1997),Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine.Leder 48,(5),99-104[304 Stainless Steel Coil Lowes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System]

Popular Laser Cutting Engraving Machine for Nonmetals, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Welding Structure.Carboxymethyl Cellulose CAS No. 9004-32-4. USP/SP[Really Microfiber PVC Leather for Sofa Furniture]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC 3 Axis Milling Machining Center]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Prices of 20W 40W 60W Solar Street Lights]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[SUS 316 Stainless Steel Coiled Tube with High Quality From China Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine]

Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),150kg Per Hour Frozen French Fries Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector]

Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Melt Pressure Transducer (PT133)]

Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Professional LED Display Ceramic Hair Curling Iron, Part I.CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[450ml Sublimation Stainless Steel Milk Bottle (Colorful)]

China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vulcanizing Machine /Rubber Tiles Vulcanzing Press.Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low, 175-184[Wholesale Frosted Color Spray Cactus Shape Glass Mason Jar]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field]

Whitaker,J.R.(1963),Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce. Analytical Chemistry35(12), 1950-1953[Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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