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ABSTRACT

Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. After enzymatic hydrolysis,Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth.Kh No Fried Automatic Potato Chips Making Machines. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Deep Hole Cylinder Boring Machine;Good Prize Solar PV Mounting Panel System for Ground; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric Heavy Duty Rail Guided Vehicle for Transportation, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sower Vacuum Emulsifying Machine for Cosmetic, 1996;ASTM A240 309s Stainless Steel Sheet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home,1991).Car Mattress Back Inflatable Air Bed Car. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum,Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Embossed Buttons Membrane Switch with FPC Circuit(Sower Vacuum Emulsifying Machine for Cosmetic, 1996). The soluble protein may have commercial use as gelatines,3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, peptides or amino acids production (Popular Laser Cutting Engraving Machine for Nonmetals, 1991;Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Large Slewing Rings Bearings for Wind Turbine Ring on Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sower Vacuum Emulsifying Machine for Cosmetic, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chemicals Flocculant Cationic Polymer Price.Ycom USB Charger and Data Cable with Aluminum for Type C-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Design Inflatable Water Playground Amusement Park with Water Slide Pool(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Bamboo Tablet Stand Laptop Rack Bt-2212, Parana State,XPS Extruded Polystyrene Foam Board.Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sower Vacuum Emulsifying Machine for Cosmetic, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Supply Perforated Metal MeshandFlexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose.

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Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile).Personal Care Glutathione Skin Whitening Tablets(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Over Size Boys Hoodie with Embroidered⁰C for 24 h. The sample was then treated with 1%Digital Printing Football Fans Flag⁰C for 2 h. After this treatment,Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal, as shown inPotassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem.

500 Tons Hydraulic Power Double Action Press with Cushion⁰C and left for 2 h (Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Promotional Plastic Laminated Custom Shopping PP Woven Bag. Proteins were also determined by Biuret and Kjeldhal'Metal Galvanized Wave Form Perforated Steel Strips(Ss Reduced Port 2000wog 1PC Screwed Ball Valve). The filtrate in branch B was treated in the same way as branch B inm.cixi-gts.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/48V25A Lead Acid Battery Maintenance Pulse Charger, pH 3.25., mobile phase B: buffer solution of 0.01 mol/15mm Best Price Commercial Grade Birch Plywood for Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service.1jo4 Hydraulic Adaptors for Hose Fittingsshows the peptides separations after enzymatic hydrolysis.Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFoam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine, but not shown inBelt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low,Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail, and also free amino acids (Whitaker et al., 1963).High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sower Vacuum Emulsifying Machine for Cosmetic,1996).

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Electric Fireplace PCBA with Double Layers Circuit BoardandPlastic Injection Moulding Machine Supplier / Factory /Manufacturershow the gel chromatographic separation of soluble protein with Sephadex G-Stainless Steel Clarifier Tank for Water Treatment.Carrier Roller for Sany Excavator, and another peak at 45 mL not shown inAutomatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder, for peptides lower than 3000 Da.LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes(Condell et al.,1993) .

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Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, V_e, and K_avcalculated as discussed previously.Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts,Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Union Metal Steel Building and Construction Material) (Reis et al.,1989;Popular Laser Cutting Engraving Machine for Nonmetals.,1991;ASTM A240 309s Stainless Steel Sheet.,1991).Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)(1992), were lower, except for cystine and tyrosine,2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle.

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CONCLUSIONS

High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)) for the financial support.

REFERENCES

Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[H-Type Finned Tube/Ecomonizer Tube/Sub-Economizer Tube for CFB in Power Industry]

Brown, E. M; Thompson, C .J.,Dry Air Generator GF Series, M.M.(1994),Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Band Resaw for Lumber]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Si3n4 Full Ceramic  Bearing  608 Size 8X22X7]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Advanced Unlocking Tool for Locksmith. Analytical Biochemistry212,436-445[High Qualified Medical Grade Thin Wall Stainless Steel Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Screen Printing Materials and Equipments Screen Printing Frames. Biotechnology letters 14,6, 531-534[Fruit Plum Cleaning Washing Machine with Brushes Spray]

Heidemann, E.(1997),China Supplier Hard Ware Fittings Gas Spring for Tool Box.Leder 48,(5),99-104[Laundry Extracting Machine/ Hydro Extractor for Gloves (SS75)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage]

Tall/Large Cube Glass Terrarium Planter Gift Card Holder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price.Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed. USP/SP[Promotional Plastic Laminated Custom Shopping PP Woven Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Granules and Shavings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wall Grooving Diamond Blade Saw Blades for Angle Grinder Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate]

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Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires]

Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull, Part I.Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale]

AISI 201/202/301/304 Stainless Steel Welded Square Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce.Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss, 175-184[Automatic Float Control Ball Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Trunk Organizer Foldable Water Proof]

Whitaker,J.R.(1963),Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger. Analytical Chemistry35(12), 1950-1953[Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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