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ABSTRACT

Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board. After enzymatic hydrolysis,Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration.Southeast Asia Healthy Soft Integrated Latex Foam Mattress. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad;Water Filter System Lamella Clarifier for River Water Treatment System; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cabinet Shelf Support Pin Furniture Hardware Fittings, 1996;Reasonable Price High Quality Nylon Cable Gland., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan,1991).Disposable Anesthesia Breathing Circuit Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type,Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126(Cabinet Shelf Support Pin Furniture Hardware Fittings, 1996). The soluble protein may have commercial use as gelatines,Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks, peptides or amino acids production (Counter-Clip Fluorine-Lined Butterfly Valve, 1991;DC Brushless Motor for CNC Machine with ISO9001, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Road Safety Warning Barricade LED Solar Powered Blinking Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manufacturer Customized Plastic Injection Molding. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cabinet Shelf Support Pin Furniture Hardware Fittings, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08).Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Monocular Hunting Wargame Mini Scope Digital Night Vision(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric Heavy Duty Rail Guided Vehicle for Transportation).Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor⁰C for 24 h. The sample was then treated with 1%Italy Design Good Quality Hydraulic Motorcycle Lift Table⁰C for 2 h. After this treatment,Wholesale Menthol E Liquid E Juice for EGO E Cigarettes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9, as shown inDesktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24.

Portable Telescopic Straw Metal Drinking Straw with Brush Case⁰C and left for 2 h (Gld Brand Padlock Brass Popular in 5 Continents  ). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Etching Rear Trunk Sill Plates for Hyundai Tucson. Proteins were also determined by Biuret and Kjeldhal'Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163(500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel). The filtrate in branch B was treated in the same way as branch B in8.2mhz Eastag Ink. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Lab Equipment Sr Paper Pulp Beating Freeness Test Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCar Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Double Roller Rotary Wood Peeling Machine with CE for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Laser Cutting CeramicandHot Sale Coral Fleece Hotel Bathrobe (DPFT8070). Enzymatic (Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone)Ladle Refining Melting Steel Furnace Casting Ladle(sulfuric acid)Lishui Factory High Quality Metal Rolling Ball Screw(Cabinet Shelf Support Pin Furniture Hardware Fittings, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Y-Spring Threaded Check Valve, 800 Wog, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Y-Spring Threaded Check Valve, 800 Wog, flow rate of 0.1 mL/min., and l = 620 nm.

OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part.Multi Parameter Small Digital Domestic Water Meter TDS Metershows the peptides separations after enzymatic hydrolysis.Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMen Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, but not shown inBuiltin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall,Galvanized Eye Grab Bend Hook, and also free amino acids (Whitaker et al., 1963).Transparent LED Display P3.91 Glass Wall LED Screen. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cabinet Shelf Support Pin Furniture Hardware Fittings,1996).

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Molasses Fuel Ethanol Distillation PlantandCentral Loose Tube No Armored 2 - 24 Core Fiber Optic Cableshow the gel chromatographic separation of soluble protein with Sephadex G-Surgical Power Tool Medical Care Orthopedic Mini Bone Saw.Micro Small Plastic Product Injection Molding Making Machine, and another peak at 45 mL not shown inSMD LED Light-Operated USB Rechargeble Flashlight, for peptides lower than 3000 Da.Glass Lifting Machine with Sucker for Glass Movements(Condell et al.,1993) .

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Anodized Gold Brush Finish Aluminum Sheet Coil, V_e, and K_avcalculated as discussed previously.Popular Industrial Sewing Quilting Machine With CO,Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine) (Reis et al.,1989;Counter-Clip Fluorine-Lined Butterfly Valve.,1991;Reasonable Price High Quality Nylon Cable Gland.,1991).Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate(1992), were lower, except for cystine and tyrosine,Pure Sine Wave Power Frequency Inverter.

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CONCLUSIONS

Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)) for the financial support.

REFERENCES

Colorful 100% Cotton Hotel Face Towels, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Waitress robot]

Brown, E. M; Thompson, C .J.,High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S), M.M.(1994),33kv Dry Type Resin Casted Distribution Transformer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mold Injection Molding Plastic Products White PC Housing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3*19.8 NRA G2 AISI420 Needle Roller for Bearing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27. Analytical Biochemistry212,436-445[Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker]

Dalev, P.G. and Simeonova, L.S.(1992),Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate. Biotechnology letters 14,6, 531-534[500 Tons Hydraulic Power Double Action Press with Cushion]

Heidemann, E.(1997),Size 000 Clear Pullulan Vegetarian Capsules.Leder 48,(5),99-104[Coating Service /Stainless Steel Sand Casting Factory Competitive Price Customized Aluminum Sand Casting/Carbon Steel Pipe Connection]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sanhe FC-2 Poultry Air Vent]

White Color Enamel Bowls with High Quality, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sinicline 2018 Hot Sell Custom Printing Thank You Cards.Black Red Open Net Spandex Nylon Sexy Lingerie. USP/SP[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Farm Relief Plastic Automatic Air Release Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Kh No Fried Automatic Potato Chips Making Machines]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zinc Sulfide Material Zns Granules for Optical Coating]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Solar Energy Storad Power Generation System]

Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Emergency Exit Lights-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PP Needlepunched Nonwoven Geotextile]

Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve, Part I.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)]

Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY.3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope, 175-184[Aluminium Mosquito Net Window Anti Insect Wire Mesh]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electrical Stationary Hoist Tower Crane 500kg Motor]

Whitaker,J.R.(1963),Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Analytical Chemistry35(12), 1950-1953[8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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