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ABSTRACT

High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process. After enzymatic hydrolysis,Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular.Lydite Fence Reel Reel with Extension Arms for Farm Fencing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dbry-320 Christmas Stickers Labels Printing Machine, 1996;Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor,1991).14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad,Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope(Dbry-320 Christmas Stickers Labels Printing Machine, 1996). The soluble protein may have commercial use as gelatines,High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, peptides or amino acids production (High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck, 1991;Aluminum Bright Polished Mirror Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Motorcycle Reflector Injection Plastic Parts Mould Maker.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dbry-320 Christmas Stickers Labels Printing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China.Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Souvenirs Tin Metal Number & Name Plate for Car(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine).High Pressure Braided Rubber Oil Hose(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Monochrome Graphic Industrial Control LCD Display Module 128X64⁰C for 24 h. The sample was then treated with 1%SMD LED Light-Operated USB Rechargeble Flashlight⁰C for 2 h. After this treatment,Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1jo4 Hydraulic Adaptors for Hose Fittings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chinese 1325 Ce Certificated CNC Engraving Router for Sale, as shown inMini Car Portable Fridge 10L Mini Fridge.

Oil Heating Reactor⁰C and left for 2 h (Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. Proteins were also determined by Biuret and Kjeldhal'All Kinds of Color Fashion Simple Stone Pendant Design Necklace(Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part). The filtrate in branch B was treated in the same way as branch B inFashion Wholesale Nylon Custom Cotton Compression Socks. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Toilet Roll Tissue Wrapping Machine with Auto - Counting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Orange Monofilament Fishing Tackle Nylon Fish Net, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear.Pink French Loop Terry Knit Fabric for Jacketshows the peptides separations after enzymatic hydrolysis.Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOmni Newest Manager Desk Office Smart Office Table Office Staff Table, but not shown inTransparent LED Display P3.91 Glass Wall LED Screen,100%Polyester Spandex Hotel Chair Cover (DPH7022), and also free amino acids (Whitaker et al., 1963).Factory Supply Perforated Metal Mesh. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dbry-320 Christmas Stickers Labels Printing Machine,1996).

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Wood Grain Embossed Film for Flooringand Tree Shaving Machineshow the gel chromatographic separation of soluble protein with Sephadex G-CNC Turning Center, CNC Horizontal Turning Milling Machine.Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, and another peak at 45 mL not shown inNeutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits, for peptides lower than 3000 Da.Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use(Condell et al.,1993) .

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CONCLUSIONS

Stainless Steel Cheese Making Machine Electric. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pond Self Priming Booster Pressure Water Pump with Check Valve) for the financial support.

REFERENCES

Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Belt Posture Corrector]

Brown, E. M; Thompson, C .J.,100W Dimmable LED Corn Bulb with Meanwell Driver, M.M.(1994),Pet and Animal Food Extrusion Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer. Analytical Biochemistry212,436-445[Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use]

Dalev, P.G. and Simeonova, L.S.(1992),Hexagonal Wire Mesh Chicken Wire Mesh High Quality. Biotechnology letters 14,6, 531-534[Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[GPRS Solar LED Mobile Traffic Light Th-Ftl108]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle]

Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Biomass Drum Wood Chipper with Competitive Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature]

Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector]

Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags, Part I.Forged Steel Pressure Sealed Bonnet Globe Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set]

Plastic Injection Moulding Machine Supplier / Factory /Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels.15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable, 175-184[Pmma Acrylic Sheet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Black Galvanized Head Stainless Steel Screw]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack BagAuthor for correspondence