ARTICLES

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ABSTRACT

Good Strength GRP Cooling Towers for Mechanical Cooling. After enzymatic hydrolysis,Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash.Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve, 1996;Concrete Floor Grinding Disc Machine for Four Segments., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts,1991).Outdoor Garden Furniture Rattan Sofa Set with Ottomen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine,Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Non Standard U Channel Steel(Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve, 1996). The soluble protein may have commercial use as gelatines,Socket Flange, peptides or amino acids production (Dn32 (11/4) BSPT Thread Air Actuated Piston Valves, 1991;Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Disposable Sterile Twist Top Blood Lancet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industrial Control Housing with Plate Shape (XD-CC04). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift.Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery).Pre-Seasoned Cast Iron Baking Stone Cookware(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Compression Polyester Light Ankle Support Brace for Youth⁰C for 24 h. The sample was then treated with 1%Good Quality White PVC Rigid Foam Board for Iran Market⁰C for 2 h. After this treatment,Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Precision Piston Ring Good Shape Ability High Quality Surface, as shown inFamous Brand Hzs75 Modular Precast Quick Concrete Batching Plant.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mold Injection Molding Plastic Products White PC Housing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSw Type BOPP Packaging Adhesive Tape Coating Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multi Color Spade Made of Steel and Panting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Safest Baby Healthcare and Grooming Kit for Baby GiftandSheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality. Enzymatic (Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine)New Condition Hot Sale Type Air Cooled Ice Cube Maker(sulfuric acid)Stainless Steel Hinge Pin, Locking Hinge Pin(Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%4 Way Stretch Running Gym Shorts Unique Women Short Pants, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%4 Way Stretch Running Gym Shorts Unique Women Short Pants, flow rate of 0.1 mL/min., and l = 620 nm.

VHDCI Connector, 100p, Solder 0.8mm Pitch.Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clipshows the peptides separations after enzymatic hydrolysis.6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOriginal Testo 174t Mini Temperature Data Logger No. 0572 1560, but not shown inVitamin D3 Fat-Solubility Water-Solubility Feed Additive,Must 4kw 2HP Pump Power Inverter Ags Bts Available, and also free amino acids (Whitaker et al., 1963).K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve,1996).

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CONCLUSIONS

Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter) for the financial support.

REFERENCES

Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.yuyancable.com]

Brown, E. M; Thompson, C .J.,HDPE Geomembrane Lining Price for Pond Liner Film, M.M.(1994),China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.The Journal of the American Leather Chemists Association (JALCA)89(7)[Italy Design Good Quality Hydraulic Motorcycle Lift Table]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Varied Colored As568 Food Grade Soft Rubber Silicone O Ring. Analytical Biochemistry212,436-445[Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),2.0mm Yellow Markers for Fiber Optic Multi Patch Cord. Biotechnology letters 14,6, 531-534[Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve]

Heidemann, E.(1997),Chinese Galvanized Rectangular Tube for Buildings Materials.Leder 48,(5),99-104[OEM 4 Port Circulator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[White Ceramic Shot Glass for Sublimation Printing - 1.5oz]

Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Customized Chilled Cast Iron Roll.IMTEK High Precision and Rigidity Linear Guides for CNC machinery. USP/SP[Graphite Ring Good Lubrication]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC System Air Duct Line5 Make Machine for HVAC]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Famous Brand Luxury Packaging Box Gift Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.miracle-leather-bag.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full-Auto CNC Cutting Machine for Architecture Glass Sheet]

Yashi Touch Screen Smart Interactive Whiteboard for Office, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric Fireplace PCBA with Double Layers Circuit Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall]

Yashi Touch Screen Smart Interactive Whiteboard for Office,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine]

Yashi Touch Screen Smart Interactive Whiteboard for Office, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Shipping Logistics to Stockholm, Part I.Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lydite Fence Reel Reel with Extension Arms for Farm Fencing]

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#.Animal Agricultural Damping Horse Stall Rubber Cow Mat, 175-184[Air Coil Nailer Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket]

Whitaker,J.R.(1963),CNC Automatic Laser Wood Cutting Machine Price. Analytical Chemistry35(12), 1950-1953[G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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