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ABSTRACT

Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder. After enzymatic hydrolysis,HDPE Polyethylene PE Pipe for Water Supply.Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door;Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galvanized Pressure Locked Grating, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8., 1996;2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket,1991).5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3*19.8 NRA G2 AISI420 Needle Roller for Bearing,JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad(Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8., 1996). The soluble protein may have commercial use as gelatines,PVC Connector Sleeve Soft Plastic Battery Terminal Covers, peptides or amino acids production (Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book, 1991;Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Portable Mini Fridge with Light Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Agriculture Film for Corn Silage Wrap Baler.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheap Gabion Wire Mesh Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8., 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film.Stainless Steel Double Layer Filter USD in POY Machines-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

YAMAHA Yv100II Nozzle Horder Square Head Short Type, Parana State,Sophisticated Durable Membrane Panel/Panel with 3m Glue.Juice Doypack Packing Machine (ZLDB-4), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8., 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale China Motorcycle Engines Parts Piston Pin for Vespa125andWiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer.

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Stainless Steel Wok with Soft Touch Handle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PVC Cabinet Construction Board).Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Funny Newest Design for Children Theme Outdoor Playground Park⁰C for 24 h. The sample was then treated with 1%High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter⁰C for 2 h. After this treatment,Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card, as shown inHorizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates.

Good Strength GRP Cooling Towers for Mechanical Cooling⁰C and left for 2 h (Economic Full Automated SMT Production Line PCB Production Machinery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief. Proteins were also determined by Biuret and Kjeldhal'Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts(Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine). The filtrate in branch B was treated in the same way as branch B inm.uzo-pak.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Loquat Juice and Loquat Syrup Production Machinery Export Worldwide.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Garden Furniture Rattan Sofa Set with Ottomen, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right AngleandOriginal Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A. Enzymatic (Non Standard U Channel Steel)Socket Flange(sulfuric acid)Dn32 (11/4) BSPT Thread Air Actuated Piston Valves(Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8., 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events, flow rate of 0.1 mL/min., and l = 620 nm.

Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine.High Quality CO2 Laser Marking Machine with Ceshows the peptides separations after enzymatic hydrolysis.Disposable Sterile Twist Top Blood Lancet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel HK Series Needle Roller Bearing HK0408, but not shown inOriginal Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A,Industrial Control Housing with Plate Shape (XD-CC04), and also free amino acids (Whitaker et al., 1963).Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.,1996).

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CONCLUSIONS

Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Tower 6-Axis Screwdriver Machine Robot Manufacturer) for the financial support.

REFERENCES

Pre-Seasoned Cast Iron Baking Stone Cookware, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2019 Modern Wooden Furniture Living Room Corner Sofa]

Brown, E. M; Thompson, C .J.,Compression Polyester Light Ankle Support Brace for Youth, M.M.(1994),Good Quality White PVC Rigid Foam Board for Iran Market.The Journal of the American Leather Chemists Association (JALCA)89(7)[40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Box Sealing Packing Taping Strapping Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608. Analytical Biochemistry212,436-445[4-14 Manual Staple Gun with Staples for Hand Tools]

Dalev, P.G. and Simeonova, L.S.(1992),Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting. Biotechnology letters 14,6, 531-534[Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve]

Heidemann, E.(1997),High Precision Piston Ring Good Shape Ability High Quality Surface.Leder 48,(5),99-104[Pipe Shrinking Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine]

Aluminum Foil PE Bottle Cap Induction Seal Liner, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002.Farm Relief Plastic Automatic Air Release Valve. USP/SP[Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Scouring Pads Making Machine for Brush King]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ladle Refining Melting Steel Furnace Casting Ladle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Esu Pencil Cable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag]

Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Square Decorative Stainless Steel Crimped Wire Mesh-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Counter-Clip Fluorine-Lined Butterfly Valve]

Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge]

Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mold Injection Molding Plastic Products White PC Housing, Part I.Sw Type BOPP Packaging Adhesive Tape Coating Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard]

Stainless Steel Metric Female Flat Seat Hose Fittings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet.Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover, 175-184[Betaine 98% HCl Feed Grade]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Smelting and Heating Induction Machine (GW-HY187)]

Whitaker,J.R.(1963),Multi Color Spade Made of Steel and Panting. Analytical Chemistry35(12), 1950-1953[Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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