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ABSTRACT

Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder. After enzymatic hydrolysis,Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery.Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Groove Type Wood Log Tree Peeling and Debarking Machine;High Quality CE Digital Display Adjustable Power Supply; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Parcel Tape Adhesive OPP Packing Tape, 1996;High Efficiency Non-Expensive Cracking Agent., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fiber Optic Sc APC Fast Connector for FTTH Drop Cable,1991).Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Striped Compression Soccer Socks for Adults,Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen(Parcel Tape Adhesive OPP Packing Tape, 1996). The soluble protein may have commercial use as gelatines,High Tensile Strength Galvanized Steel Wire, peptides or amino acids production (24 Volt Fire Alarm Strobe Light Horn Strobe Sounder, 1991;from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Price PVC Coated Fiberglass Mesh Insect Window Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Parcel Tape Adhesive OPP Packing Tape, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory OEM CNC Machined Gear Motor Shaft.High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pink French Loop Terry Knit Fabric for Jacket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine).Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cute Pink Cashmere Soft Plush Lining Warm Bootie⁰C for 24 h. The sample was then treated with 1%3kw 24V 310mm Vertical Agv Drive Wheel Assembly⁰C for 2 h. After this treatment,Sofa Materials PU Leather with Flock Backing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Three Phase 800mm Plate Fan's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ASTM Approved Popular Indoor Trampoline of Park, as shown in with Handle Vegetable Oil Nonstick.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMen Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Flat Car Sun Roof Tent Awning⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Classic Black Color Commercial Glass Door Multi Deck Refrigerator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel) for the financial support.

REFERENCES

Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.xsz-cnc.com]

Brown, E. M; Thompson, C .J.,Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800, M.M.(1994),Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou. Analytical Biochemistry212,436-445[High Quality CO2 Laser Marking Machine with Ce]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck. Biotechnology letters 14,6, 531-534[Stainless Steel HK Series Needle Roller Bearing HK0408]

Heidemann, E.(1997),Aluminum Bright Polished Mirror Sheet.Leder 48,(5),99-104[Online Hot Sale Benzyl Benzoate / Bb From China Factory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A]

Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open).Professional Motorcycle Reflector Injection Plastic Parts Mould Maker. USP/SP[Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Glass Cutting Table/CNC Glass Cutting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Double Side Hair Twist Curl Sponge Safe Hair Twist Sponge Brush for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Laptop Bag College Men Backpack Leather Back Pack Bags]

Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pet Dog Grooming Rake Dematting Tool Shedding Comb]

Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Adhesive CAS 9003-35-4 Phenolic Resin]

Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Souvenirs Tin Metal Number & Name Plate for Car, Part I.AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad]

Molasses Fuel Ethanol Distillation Plant, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pond Self Priming Booster Pressure Water Pump with Check Valve.Banded Type Tee Used on Oil, 175-184[UL 854 Service Entrance Cable Aluminum/Copper Type Se]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette]

Whitaker,J.R.(1963),20FT New Shipping Container 40FT Dry Cargo Container. Analytical Chemistry35(12), 1950-1953[40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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