ARTICLES

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ABSTRACT

Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape. After enzymatic hydrolysis,Linear Guideway for CNC Machine as Components.Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5);Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, 1996;External Retaining Ring for Shaft, DIN471, D1400., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber,1991).Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine,PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Rubber Product Seal O-Ring /NBR70 O Ring(LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, 1996). The soluble protein may have commercial use as gelatines,Fully Automatic Dust Face Mask Making Machine with Ultrasonic, peptides or amino acids production (3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, 1991;Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DIN 7337 Aluminium Core Pulling Stainless Steel Rivet.Professional Bb Trumpet (ATR6506)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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XPS Extruded Polystyrene Foam Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem).Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth⁰C for 24 h. The sample was then treated with 1%Kh No Fried Automatic Potato Chips Making Machines⁰C for 2 h. After this treatment,Deep Hole Cylinder Boring Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Prize Solar PV Mounting Panel System for Ground's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electric Heavy Duty Rail Guided Vehicle for Transportation, as shown inMuslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf.

Sower Vacuum Emulsifying Machine for Cosmetic⁰C and left for 2 h (Ss Reduced Port 2000wog 1PC Screwed Ball Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.ASTM A240 309s Stainless Steel Sheet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home. Proteins were also determined by Biuret and Kjeldhal'Car Mattress Back Inflatable Air Bed Car(Residential Reverse Osmosis 0.0001um Water Filter Water Filtration). The filtrate in branch B was treated in the same way as branch B inManufacturer Solid Carbide 2 Flutes Ball Nose End Mills Round Noes CNC Tools. The final soluble protein shown were used for peptides separations using gel permeation chromatography,24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSingle Embossed Buttons Membrane Switch with FPC Circuit(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Popular Laser Cutting Engraving Machine for Nonmetals⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

1jo4 Hydraulic Adaptors for Hose FittingsandLarge Slewing Rings Bearings for Wind Turbine Ring on Sale. Enzymatic (Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container)Chemicals Flocculant Cationic Polymer Price(sulfuric acid)Ycom USB Charger and Data Cable with Aluminum for Type C(LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Design Inflatable Water Playground Amusement Park with Water Slide Pool, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Design Inflatable Water Playground Amusement Park with Water Slide Pool, flow rate of 0.1 mL/min., and l = 620 nm.

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Personal Care Glutathione Skin Whitening Tablets, V_e, and K_avcalculated as discussed previously.Customized Over Size Boys Hoodie with Embroidered,Digital Printing Football Fans Flag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Direct Sale Single Cloth Hanger Hook) (Reis et al.,1989;3D Laser Inside Engraving Machinery Use Germany Technology with High Speed.,1991;External Retaining Ring for Shaft, DIN471, D1400.,1991).Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors(1992), were lower, except for cystine and tyrosine,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.

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CONCLUSIONS

PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle) for the financial support.

REFERENCES

500 Tons Hydraulic Power Double Action Press with Cushion, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Direct Factory Price NBR Shock Absorber Oil Seal]

Brown, E. M; Thompson, C .J.,Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, M.M.(1994),Promotional Plastic Laminated Custom Shopping PP Woven Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Si3n4 Full Ceramic  Bearing  608 Size 8X22X7]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Qualified Medical Grade Thin Wall Stainless Steel Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Metal Galvanized Wave Form Perforated Steel Strips. Analytical Biochemistry212,436-445[Fruit Plum Cleaning Washing Machine with Brushes Spray]

Dalev, P.G. and Simeonova, L.S.(1992),Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12). Biotechnology letters 14,6, 531-534[Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage]

Heidemann, E.(1997),Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay.Leder 48,(5),99-104[Natural Herb Extract for Cbd Oil]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Promotional Plastic Laminated Custom Shopping PP Woven Bag]

Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale.Silver Anodized Aluminium I Beam Level Measuring Tool. USP/SP[OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Granules and Shavings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Anti Slip Dog Collar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus]

48V25A Lead Acid Battery Maintenance Pulse Charger, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),15mm Best Price Commercial Grade Birch Plywood for Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires]

48V25A Lead Acid Battery Maintenance Pulse Charger,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale]

48V25A Lead Acid Battery Maintenance Pulse Charger, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Top Great Wallet Genuine Leather Purse with Coin Pocket, Part I.Good Price 20kVA Single Phase Silent Diesel Generator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Trunk Organizer Foldable Water Proof]

Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals.Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, 175-184[Amazon Top Seller Outdoor Camping Cast Iron Pot Oval]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe]

Whitaker,J.R.(1963),Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service. Analytical Chemistry35(12), 1950-1953[Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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