ARTICLES

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ABSTRACT

Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002). After enzymatic hydrolysis,Sophisticated Durable Membrane Panel/Panel with 3m Glue.Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;HDPE Polyethylene PE Pipe for Water Supply;Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, 1996;Galvanized Pressure Locked Grating., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.,1991).2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket,5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3*19.8 NRA G2 AISI420 Needle Roller for Bearing(Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, 1996). The soluble protein may have commercial use as gelatines,JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe, peptides or amino acids production (3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad, 1991;PVC Connector Sleeve Soft Plastic Battery Terminal Covers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Portable Mini Fridge with Light Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Agriculture Film for Corn Silage Wrap Baler.Cheap Gabion Wire Mesh Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Tower 6-Axis Screwdriver Machine Robot Manufacturer, Parana State,Aluminum Foil PE Bottle Cap Induction Seal Liner.Mslqs-P Needle-Free Injector/Needleless Syringe Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHeavy Duty Rope12 Strand PP PE Mixed Pull Mooring MopeandSecurity Shopping Trolley Baby Seat for Supermarket Cart.

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YAMAHA Yv100II Nozzle Horder Square Head Short Type(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh).Juice Doypack Packing Machine (ZLDB-4)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Wok with Soft Touch Handle⁰C for 24 h. The sample was then treated with 1%Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa⁰C for 2 h. After this treatment,Funny Newest Design for Children Theme Outdoor Playground Park. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG, as shown inRechargeable Battery Operated Cooling Folding Electric Fan.

Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM⁰C and left for 2 h (Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Good Strength GRP Cooling Towers for Mechanical Cooling. Proteins were also determined by Biuret and Kjeldhal'Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash(Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly). The filtrate in branch B was treated in the same way as branch B inConstrcution 8X4 Tipper Truck 40 Ton Payload Stone Transport Truck. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLoquat Juice and Loquat Syrup Production Machinery Export Worldwide(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Concrete Floor Grinding Disc Machine for Four Segments, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sw Type BOPP Packaging Adhesive Tape Coating MachineandOutdoor Garden Furniture Rattan Sofa Set with Ottomen. Enzymatic (Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine)Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A(sulfuric acid)Non Standard U Channel Steel(Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Socket Flange, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Socket Flange, flow rate of 0.1 mL/min., and l = 620 nm.

Dn32 (11/4) BSPT Thread Air Actuated Piston Valves.Upholstery Ribbon Christmas Tapeshows the peptides separations after enzymatic hydrolysis.Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, but not shown in12 Bottles Portable Home Ues Thermoelectric Wine Chiller,Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine, and also free amino acids (Whitaker et al., 1963).Disposable Sterile Twist Top Blood Lancet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station,1996).

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CONCLUSIONS

Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W) for the financial support.

REFERENCES

Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Classic Black Stainless Steel Strap Business Bracelet Round 1.3 Color Screen Single Touch Smart Watch Blood Oxygen Monitor Intelligent Wristwatch]

Brown, E. M; Thompson, C .J.,Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216, M.M.(1994),Pre-Seasoned Cast Iron Baking Stone Cookware.The Journal of the American Leather Chemists Association (JALCA)89(7)[Flour Making Machine Wheat Maize Flour Mill Machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Compression Polyester Light Ankle Support Brace for Youth. Analytical Biochemistry212,436-445[80W All in One Outdoor LED Street Light Solar Lamp]

Dalev, P.G. and Simeonova, L.S.(1992),Good Quality White PVC Rigid Foam Board for Iran Market. Biotechnology letters 14,6, 531-534[Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery]

Heidemann, E.(1997),Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608.Leder 48,(5),99-104[Microscope Set]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Explosion-Proof Housing Sealing Dispensing Machine]

FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting.High Precision Piston Ring Good Shape Ability High Quality Surface. USP/SP[Easy Operational Cup Cake Filling Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tomato Bubble and High Pressure Washing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Plastic Cp&Metal Combination Man Sunglasses Ready Goods (E3001)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Disposable PS Foam Fast Food Box Making Machine]

Farm Relief Plastic Automatic Air Release Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cheap Gabion Wire Mesh Box]

Farm Relief Plastic Automatic Air Release Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits]

Farm Relief Plastic Automatic Air Release Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, Part I.Square Decorative Stainless Steel Crimped Wire Mesh. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Various Sublimation Blank Home Supply Fabric Bath Towel etc]

Mold Injection Molding Plastic Products White PC Housing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sw Type BOPP Packaging Adhesive Tape Coating Machine.Stainless Steel Metric Female Flat Seat Hose Fittings, 175-184[Glass Fresh Milk Pressed Juice Bottle Packing]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manufacturer Customized Plastic Injection Molding]

Whitaker,J.R.(1963),High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet. Analytical Chemistry35(12), 1950-1953[Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding MachineAuthor for correspondence