ARTICLES

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ABSTRACT

Graphic 128X128 Dots Cog LCD. After enzymatic hydrolysis,Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen.Tcn Sex Toy Vending Machines for Sale S800-10. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Silver 8 Inch Double Thick Round Cake Board;High Precision Machining Auto Parts Metal Contact; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, 1996;Paper Sticky Lint Remover Roller/Pet Hair Roller., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Safe Durable Scaffolding Trapdoor Plank for Construction,1991).China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Various Sublimation Blank Home Supply Fabric Bath Towel etc,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments(Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, 1996). The soluble protein may have commercial use as gelatines,Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, peptides or amino acids production (Best Quality Bending Metal Mould with All Kinds Metal Materials, 1991;New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Milling Precision Stainless Steel CNC Machinery Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Price Stand-up Zipper Doy Pouch Filling Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21.Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dxz Limit Switch Tower Crane Spare Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Farrowing Crate Pig Floor Cast Iron Slat Floor).OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High-End Business Magnet Leather Ring Binder Notebook⁰C for 24 h. The sample was then treated with 1%Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops⁰C for 2 h. After this treatment,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Eyes- Caring LED Night Light for Nursing Reading with Soft Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Bearing with Housing Bearing / Pillow Block Bearing, as shown inAmazon Armor TPU Silicone Phone Case for Honor 10 Lite.

Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800⁰C and left for 2 h (China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.The Most Competitive Sodium Gluconate for Water Reducer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles. Proteins were also determined by Biuret and Kjeldhal'Electric Magic Hot Hair Styling Curling Iron with Brushes(5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes). The filtrate in branch B was treated in the same way as branch B in3D Big Large Roll Sale Factory Modern High Quality Living Room Geometric Nylon Printed Rug. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Melt Pressure Transducer (PT133).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWomen Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Lady Kids Child Yoga Clothes Gym Fitness Sports Wear.Power Fitting- Hot DIP Galvanizing Bolt with Nutshows the peptides separations after enzymatic hydrolysis.Hotel Steel Digital Room Safes with Large LED Display(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, but not shown in Shampoo Mascara Titling Mixing and Emulsifying Making Machine,Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, and also free amino acids (Whitaker et al., 1963).Biogas/Methane Purification System/Desulfurization/Scrubber Tower. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler,1996).

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CONCLUSIONS

Flow Wrapper/ Bakery Moon Cake Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Christmas Decoration Rose Flower LED Light String) for the financial support.

REFERENCES

Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Integrated Style Ball Valve Pn1.6]

Brown, E. M; Thompson, C .J.,Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, M.M.(1994),Reusable Metal Stainless Steel Drinking Straws.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Price 20kVA Single Phase Silent Diesel Generator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Designed Steel Frame Factory Building. Analytical Biochemistry212,436-445[Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0]

Dalev, P.G. and Simeonova, L.S.(1992),Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate. Biotechnology letters 14,6, 531-534[Mushroom Head Stud Sert]

Heidemann, E.(1997),0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table.Leder 48,(5),99-104[Aluminum Milling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Glass Mix Stone Mosaic Tiles: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Push Pull Small Cable to Cable Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Toothbrush Rack Bathroom Shelf Free Punching Wall-Absorbing Toilet Wash Set Dou Yin Artifact Automatic Squeeze Toothpaste]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Elegant Europe Dessign Pillow Top Mattress]

Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable]

Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ASTM Approved Popular Indoor Trampoline of Park]

Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, Part I.Used/Second Hand 120mpm Continous Galvanizing Line/Cgi. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Factory Inline Hockey Skate Puck]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Price Custom Embroidery Logo Hotel Slippers]

Whitaker,J.R.(1963),High Quality Stainless Steel Expanded Metal Mesh. Analytical Chemistry35(12), 1950-1953[New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Food Metal Detector Machine for Production LineAuthor for correspondence