ARTICLES

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ABSTRACT

Pond Self Priming Booster Pressure Water Pump with Check Valve. After enzymatic hydrolysis,Banded Type Tee Used on Oil.20FT New Shipping Container 40FT Dry Cargo Container. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover;Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Pressure Braided Rubber Oil Hose, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Monochrome Graphic Industrial Control LCD Display Module 128X64, 1996;SMD LED Light-Operated USB Rechargeble Flashlight., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden,1991).1jo4 Hydraulic Adaptors for Hose Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chinese 1325 Ce Certificated CNC Engraving Router for Sale,Oil Heating Reactor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator(Monochrome Graphic Industrial Control LCD Display Module 128X64, 1996). The soluble protein may have commercial use as gelatines,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, peptides or amino acids production (All Kinds of Color Fashion Simple Stone Pendant Design Necklace, 1991;Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Monochrome Graphic Industrial Control LCD Display Module 128X64, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Toilet Roll Tissue Wrapping Machine with Auto - Counting.Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Orange Monofilament Fishing Tackle Nylon Fish Net(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Evaporator Finned Tube, Parana State,Molasses Fuel Ethanol Distillation Plant.Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Monochrome Graphic Industrial Control LCD Display Module 128X64, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLow Carbon Heavy Galvanized Steel Wireand50Hz to 60Hz Frequency Inverter for Water Pump.

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Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber).100%Polyester Spandex Hotel Chair Cover (DPH7022)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Supply Perforated Metal Mesh⁰C for 24 h. The sample was then treated with 1%CNC Turning Center, CNC Horizontal Turning Milling Machine⁰C for 2 h. After this treatment,Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, as shown inHot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens.

Vitamin D3 Fat-Solubility Water-Solubility Feed Additive⁰C and left for 2 h (High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Wholesale Zinc Plated Carbon Steel Hex Nut. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. Proteins were also determined by Biuret and Kjeldhal'Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V(Structural Synthetic Fiber for Concrete). The filtrate in branch B was treated in the same way as branch B in20-63mm Ppr Pipe Extrusion Line. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Cheese Making Machine Electric.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100W Dimmable LED Corn Bulb with Meanwell Driver(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hexagonal Wire Mesh Chicken Wire Mesh High Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/V-32 New Special UPS Solar Battery Cabinet Box Case, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Sanhe FC-2 Poultry Air Vent. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Diameter 1300mm Lifting Electro Magnet for Cast Iron) for the financial support.

REFERENCES

Picosecond Laser Tattoo Removal Skin Care Medical Instrument, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Support Jack Stands]

Brown, E. M; Thompson, C .J.,XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, M.M.(1994),Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849.The Journal of the American Leather Chemists Association (JALCA)89(7)[Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Humidity Refrigeration Digital Controller Jsd-100+]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart. Analytical Biochemistry212,436-445[6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)]

Dalev, P.G. and Simeonova, L.S.(1992),SUS316 Hexagon Socket Set Screws with Cone Point, DIN914. Biotechnology letters 14,6, 531-534[Nylon Weatherproof PA6 Strain Relief Cable Gland]

Heidemann, E.(1997),Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale.Leder 48,(5),99-104[Ppopular Glass Coffee Table Desig for Wholesale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Bright Polished Mirror Sheet]

Customized Souvenirs Tin Metal Number & Name Plate for Car, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor.Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC. USP/SP[Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electrical Barber Shop Equipment Electric Hair Curling Wand Iron]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Explosion-Proof Housing Sealing Dispensing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[HDPE Polyethylene PE Pipe for Water Supply]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hospital Medical Waste Waste Burning Stove]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chinese Supplier of PVC Profile]

Tube Mills for Thin Wall Tubing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Non Rechargeable]

Tube Mills for Thin Wall Tubing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)]

Tube Mills for Thin Wall Tubing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Car Badge Custom Emblem Auto Logo Sign for Advertising, Part I.2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles]

Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006.Rear Wheel Exchangeable Aluminum Manual Wheelchair, 175-184[200 X 100 X 10mm Thick En10219 S355j2h Carbon Steel Rectangular Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls]

Whitaker,J.R.(1963),90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow. Analytical Chemistry35(12), 1950-1953[Professional Bb Trumpet (ATR6506)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Vacuum Coating Machine Water Chiller (CW-6300AN)Author for correspondence