ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-1.6 Ton Hand Lifting Tool Lever Chain Block. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CNC Machining Service High Precision CNC Mechanical Parts

ABSTRACT

Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks. After enzymatic hydrolysis,Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker.Printed 100% Polyester Tcheap Wholesale Blankets. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Food Grade Material 304/316 Double Diaphragm Pumps;Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Power From Han′s GS 2000W Laser Cutter, 1996;Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Widely Application Latest Designed Coffee Capsule Filling Packing Machine,1991).Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,18V Lithium Battery Powered Cordless Drill,High Density Rock Wool Wall Board with Thermal Insulation Performance,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh(High Power From Han′s GS 2000W Laser Cutter, 1996). The soluble protein may have commercial use as gelatines,Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, peptides or amino acids production (China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery, 1991;China PAC Water Flocculating Agent for Water Treatment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Price High Carbon Isostatic Graphite Electrodes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Power From Han′s GS 2000W Laser Cutter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light.Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Powder/Spices Powder Packing Machine with Four/Three Side Seal(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Black Phosphated Bugle Head Csk Head Drywall ScrewAlcalase2.5 L DX,Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

6 Inch Plastic Breathing Cap for The IBC Valve, Parana State,CNC Machining Service High Precision CNC Mechanical Parts.Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Power From Han′s GS 2000W Laser Cutter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFiberglass Animal Poultry Insulation Grain TowerandSupreme 524 Mv Sea Kayak.

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Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels).Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part⁰C for 24 h. The sample was then treated with 1%Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable⁰C for 2 h. After this treatment,Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Freshwater Pearl Butterfly Flower Bride Hairpin, as shown inFDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit.

Air Bubble Insulation Thermal Building Insulation Material⁰C and left for 2 h (13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rechargeable Battery Operated Cooling Folding Electric Fan. Proteins were also determined by Biuret and Kjeldhal'High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve(8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light). The filtrate in branch B was treated in the same way as branch B inBreathing Mask For Running. The final soluble protein shown were used for peptides separations using gel permeation chromatography,21.5′′ Wall Mount Display Capacitive Touch Monitor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pagoda Gazebo Tent in Guangzhou for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Black Small Fridge for Showcase⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round PipeandZircon Butterfly Open Ring Diamond Fashion Woemn Rings. Enzymatic (High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation)Spare Parts Custom Injection Plastic Moulding Manufacturer(sulfuric acid)Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill(High Power From Han′s GS 2000W Laser Cutter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood, flow rate of 0.1 mL/min., and l = 620 nm.

Kingdomway Brand Vitamin a 1000.Diameter 1300mm Lifting Electro Magnet for Cast Ironshows the peptides separations after enzymatic hydrolysis.Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), but not shown inFoshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration,High Speed Roll Plastic T-Shirt Bag Making Machine, and also free amino acids (Whitaker et al., 1963).Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Power From Han′s GS 2000W Laser Cutter,1996).

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CONCLUSIONS

Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump) for the financial support.

REFERENCES

Factory Price DIN5685 Galvanized Marine Anchor Link Chain, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fire Proof Doors]

Brown, E. M; Thompson, C .J.,Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), M.M.(1994),6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hwf Tsp Core Bit for Geotechnical Drilling]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Level Application Waterproof Plastic Box. Analytical Biochemistry212,436-445[Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine. Biotechnology letters 14,6, 531-534[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

Heidemann, E.(1997),Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse.Leder 48,(5),99-104[Loose Plate Flange]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[11kg 24L Low Pressure LPG Gas Cylinder]

Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Water Slide for Indoor Play Structure.Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. USP/SP[Aluminum Forging Cast Metal Sand Die Casting]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1220*2440mm Concrete Formwork black film faced plywood: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Sales Marble Sculpture Home Decoration Beauty Bust Statue Mfsg-77]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder]

Washami Teeth Care for Whitening Cool Mint Toothpaste, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming]

Washami Teeth Care for Whitening Cool Mint Toothpaste,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dn32 (11/4) BSPT Thread Air Actuated Piston Valves]

Washami Teeth Care for Whitening Cool Mint Toothpaste, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, Part I.SHXJ1500 CNC Diamond Wire Stone Cutting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall]

Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 Foam Concrete Machine/Foamed Concrete Block Making Machine.Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, 175-184[m.damai-grinder.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chicken Cage Mesh Hexagonal Wire Mesh Machine]

Whitaker,J.R.(1963),Yyh Series One Dimension Tumbling Mixer Mixing Machine. Analytical Chemistry35(12), 1950-1953[Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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