ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Aluminum Adjustable Quick Assemble Stage with Steps. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hardware CNC Machinery Milling Part

ABSTRACT

Customized 8 Layers Round LED Panel Aluminum PCB for LED Light. After enzymatic hydrolysis,Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388).Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3);Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Linear Guideway for CNC Machine as Components, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, 1996;40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r,1991).Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W,External Retaining Ring for Shaft, DIN471, D1400,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber(Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, 1996). The soluble protein may have commercial use as gelatines,Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand, peptides or amino acids production (Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine, 1991;PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Rubber Product Seal O-Ring /NBR70 O Ring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fully Automatic Dust Face Mask Making Machine with Ultrasonic.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D Laser Inside Engraving Machinery Use Germany Technology with High Speed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf.460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3.5ton Chinese Engine Gasoline Forklift (FG35T), Parana State,Hardware CNC Machinery Milling Part.Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the43 Glass Beads Cat Eye Aluminum Road StudandWater Cooled Low Temperature Screw Chiller for Industrial Workshop.

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Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag).Waterproof Covering Material Interior Wall Composite Panel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tall/Large Cube Glass Terrarium Planter Gift Card Holder⁰C for 24 h. The sample was then treated with 1%Venlo Type Flower Multi Span Commercial Glass Greenhouses Used⁰C for 2 h. After this treatment,XPS Extruded Polystyrene Foam Board. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth, as shown inGlass Lifting Machine with Sucker for Glass Movements.

Kh No Fried Automatic Potato Chips Making Machines⁰C and left for 2 h (Flat Car Sun Roof Tent Awning). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Deep Hole Cylinder Boring Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Good Prize Solar PV Mounting Panel System for Ground. Proteins were also determined by Biuret and Kjeldhal'Electric Heavy Duty Rail Guided Vehicle for Transportation(Atf-805 Auto Car Transmission System Flushing Machine). The filtrate in branch B was treated in the same way as branch B inAnti Theft Barcode Label. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sower Vacuum Emulsifying Machine for Cosmetic.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ASTM A240 309s Stainless Steel Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInvt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Single Embossed Buttons Membrane Switch with FPC Circuit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machineand3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card. Enzymatic (Popular Laser Cutting Engraving Machine for Nonmetals)Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit(sulfuric acid)Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions(Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Large Slewing Rings Bearings for Wind Turbine Ring on Sale, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Large Slewing Rings Bearings for Wind Turbine Ring on Sale, flow rate of 0.1 mL/min., and l = 620 nm.

Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container.Hwf Tsp Core Bit for Geotechnical Drillingshows the peptides separations after enzymatic hydrolysis.Chemicals Flocculant Cationic Polymer Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inin Ear Tws I9 Mini Bluetooths Earbuds with Charging Case, but not shown inHydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600,Ycom USB Charger and Data Cable with Aluminum for Type C, and also free amino acids (Whitaker et al., 1963).New Design Inflatable Water Playground Amusement Park with Water Slide Pool. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts,1996).

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11kg 24L Low Pressure LPG Gas CylinderandAluminum Forging Cast Metal Sand Die Castingshow the gel chromatographic separation of soluble protein with Sephadex G-240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface.Light Weight Polyester Eye Mask for Travel Airplane Hotel, and another peak at 45 mL not shown inHigh Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, for peptides lower than 3000 Da.6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)(Condell et al.,1993) .

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2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable, V_e, and K_avcalculated as discussed previously.Bamboo Tablet Stand Laptop Rack Bt-2212,Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder) (Reis et al.,1989;Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine.,1991;40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5).,1991).Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair(1992), were lower, except for cystine and tyrosine,Personal Care Glutathione Skin Whitening Tablets.

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CONCLUSIONS

Customized Over Size Boys Hoodie with Embroidered. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Bb Trumpet (ATR6506)) for the financial support.

REFERENCES

Digital Printing Football Fans Flag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Case]

Brown, E. M; Thompson, C .J.,Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors, M.M.(1994),Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chicken Cage Mesh Hexagonal Wire Mesh Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal. Analytical Biochemistry212,436-445[Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima]

Dalev, P.G. and Simeonova, L.S.(1992),500 Tons Hydraulic Power Double Action Press with Cushion. Biotechnology letters 14,6, 531-534[Roller Wedge Screen Cylinder]

Heidemann, E.(1997),Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine.Leder 48,(5),99-104[Factory Marble Sculpture Home Decoration Male Head Bust Statue Mfsg-72]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Freshwater Pearl Butterfly Flower Bride Hairpin]

43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Promotional Plastic Laminated Custom Shopping PP Woven Bag.Metal Galvanized Wave Form Perforated Steel Strips. USP/SP[Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Elbow Forming Machine Gorelocker HVAC Duct Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pond Copper Wire Self-Priming Auto Water Pump with Pressure Sensor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customize Milky Extrusion PC Cover]

Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair]

Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine]

Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Silver Anodized Aluminium I Beam Level Measuring Tool, Part I.Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles]

48V25A Lead Acid Battery Maintenance Pulse Charger, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),15mm Best Price Commercial Grade Birch Plywood for Furniture.Top Great Wallet Genuine Leather Purse with Coin Pocket, 175-184[M20 Carriage Bolts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)]

Whitaker,J.R.(1963),Good Price 20kVA Single Phase Silent Diesel Generator. Analytical Chemistry35(12), 1950-1953[Bathroom False Plastic Board Interior Decorative 3D Wall Panels]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Steel Wire Rope Pulling Hoist or Manual Lifting TirforAuthor for correspondence