ARTICLES

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ABSTRACT

Durable Aluminum Flag Pole with Dome Top Tangle Free Finial. After enzymatic hydrolysis,Customized Marathon Medal for Marathon Medallion.Price of a Retail Supermarket Push Cart for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder;Lady Kids Child Yoga Clothes Gym Fitness Sports Wear; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hotel Steel Digital Room Safes with Large LED Display, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, 1996;Biogas/Methane Purification System/Desulfurization/Scrubber Tower., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line,1991).Popular Custom School Uniform Woven Badge Patch with Merrow Border. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor,Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electrical Stationary Hoist Tower Crane 500kg Motor(Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, 1996). The soluble protein may have commercial use as gelatines,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, peptides or amino acids production (Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange, 1991;Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Flow Wrapper/ Bakery Moon Cake Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Reusable Metal Stainless Steel Drinking Straws.Designed Steel Frame Factory Building-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, Parana State,Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck.3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for ZimbabweandHot Sale Cute Food Plastic Pet Slow Feed Dog Bowl.

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Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Tensile Strength Galvanized Steel Wire).Used/Second Hand 120mpm Continous Galvanizing Line/Cgi(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie⁰C for 24 h. The sample was then treated with 1%Racing Preformance PTFE Teflon Alloy Fuel Adapter⁰C for 2 h. After this treatment,Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Stainless Steel Expanded Metal Mesh's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Popular Lovely Animal Design Inflatable Slide for Kids, as shown inMonochrome Graphic Industrial Control LCD Display Module 128X64.

Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware⁰C and left for 2 h (Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hairs Comb for Pet Dog Cats. Proteins were also determined by Biuret and Kjeldhal'Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall(Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts). The filtrate in branch B was treated in the same way as branch B inMIFARE DESFire EV2 4K ISO14443A RFID Card. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Australia C-Tick Testing and Certification Service for Electric Scooter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSTPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Roller Wedge Screen Cylinder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot SaleandEco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup. Enzymatic (Push Pull Small Cable to Cable Connector)Stainless Steel Seamless Pipe ASTM A312 TP304 316(sulfuric acid)Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco(Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, flow rate of 0.1 mL/min., and l = 620 nm.

Big Chocolate Storage Holding Tank, Chocolate Buffer Tank.Promotional Gifts Custom Printed Silicone Wristband/Braceletshows the peptides separations after enzymatic hydrolysis.Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inShenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, but not shown inHigh Quality Stainless Steel Plate Flange,Asco 353 Series 3/4 Pulse Valve Solenoid Valve, and also free amino acids (Whitaker et al., 1963).China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture,1996).

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CONCLUSIONS

Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor) for the financial support.

REFERENCES

Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[99% Purity of Erythromycin Thiocyanate Powder for Anti-Infective 7704-67-8]

Brown, E. M; Thompson, C .J.,Outdoor Garden Save Energy with Solar Flame Wall Light, M.M.(1994),CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).The Journal of the American Leather Chemists Association (JALCA)89(7)[Colorful 100% Cotton Hotel Face Towels]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag. Analytical Biochemistry212,436-445[Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses]

Dalev, P.G. and Simeonova, L.S.(1992),Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length. Biotechnology letters 14,6, 531-534[China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material]

Heidemann, E.(1997),Non Stick Silicone Coated Fiberglass Cloth.Leder 48,(5),99-104[Parallel Shaft Gearbox]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone]

Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502.Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius. USP/SP[Pneumatic Control Shuttle Valve NPT Thread]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Fashion PVC Sequins Adjustable Women Brace Suspenders]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Synergist for Soap; Water Softener]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Expanded Metal Mesh for Facade Cladding/Fence]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture]

DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Eye Steam Mask]

DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Carrier Roller for Sany Excavator]

DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, Part I.Hot Sale 100% Polyester Cartoon Kids Sleepwear. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot DIP Galvanized G2150 D Ring Shackle]

Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater.Laparoscopy Clip Applicator for Abdominal Surgery Uses, 175-184[function Cheap Nursing Care Bed 2 Crank Hospital Bed - 2 Crank Hospital Bed]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum Oxide Non Woven Polishing Wheel for Machine]

Whitaker,J.R.(1963),High Yield Tree Stump Crusher Machine. Analytical Chemistry35(12), 1950-1953[Concrete Floor Grinding Disc Machine for Four Segments]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic TileAuthor for correspondence