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ABSTRACT

Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch. After enzymatic hydrolysis,Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430.Polyglycolic Acid Absorable Suture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Mould Shoe Mould Making CNC Milling Router Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Pressure Fitting Quick Coupling Hydraulic Plug, 1996;High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Refrigeration Digital Temperature Controller Stc-100A,1991).Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies,Black Color Hexagon Bolt with High Quality,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes(High Pressure Fitting Quick Coupling Hydraulic Plug, 1996). The soluble protein may have commercial use as gelatines,Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price, peptides or amino acids production (Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag, 1991;High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Pressure Fitting Quick Coupling Hydraulic Plug, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM.Single Stage High Speed Fruit and Vegetable Pulping Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Movable Partition Walls for Room Division, Hotel and Shopping Mall, Parana State,T90 High Power Latching Relay Mini Power Relay.Carboxy Terminal Collagen Crosslinks Elisa Kits, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Pressure Fitting Quick Coupling Hydraulic Plug, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Synergist for Soap; Water SoftenerandVintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture.

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Various Colors Nylon and Stainless Steel Kitchen Tools(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Carrier Roller for Sany Excavator).355nm UV Laser Marking Equipment for Crystal Materials(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Metal Separating Gold Brass Zippers for Sale⁰C for 24 h. The sample was then treated with 1%Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate⁰C for 2 h. After this treatment,OEM Refrigeration Digital Thermometer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making, as shown inHot DIP Galvanized G2150 D Ring Shackle.

79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar⁰C and left for 2 h (Aluminum Oxide Non Woven Polishing Wheel for Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Two Roll Rubber & Plastic Mixing Mills Tire Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes. Proteins were also determined by Biuret and Kjeldhal'Hot Sale 25kw Grid Tied Home Solar Panel System Kit(Concrete Floor Grinding Disc Machine for Four Segments). The filtrate in branch B was treated in the same way as branch B inpersonal alarm keychain. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mini Car Portable Fridge 10L Mini Fridge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMini Small Garden Excavator Digging Work(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Offshore Drilling Platform Air Winch for Marine Oilfields⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heavy Industry Electric Transport Vehicle on Rails, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard) for the financial support.

REFERENCES

Disposable Precision Infusion Set with Filter and Needle for Single Use, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5 Gallon Pc Plastic Bottle Blow Moulding Machine]

Brown, E. M; Thompson, C .J.,Low Price Sweep Frequency Response Analyzer for Power Transformer Test, M.M.(1994),Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500.The Journal of the American Leather Chemists Association (JALCA)89(7)[Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dia. 5.5mm Bonding ND: YAG Laser Rod Lens]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Mechanic Parts for Lock Accessories with MIM Process. Analytical Biochemistry212,436-445[Rectification Triming Metallized Polyester Film Slitting Rewinding Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT. Biotechnology letters 14,6, 531-534[Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door]

Heidemann, E.(1997),Mining Machinery Sand Vibrating Sieve for River Sand.Leder 48,(5),99-104[Mv]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Palet Coil Nail Welding Machine/Nail Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hvac Control Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flat Pack Temperary Container House 20FT 40FT]

Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality MPO Singlemode Duplex Fiber Optic Adapter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Engieered Veneer Faced Commericial Plywood]

Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting]

Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hotel Bedding, Part I.Supplier V Wedge Wire Johnson Water Well Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock]

Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),10A MPPT Solar Controllers PV 60V for off-Grid.100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears, 175-184[Air Conditioner Corrugated Pipe Machinery Exporter Hot Sale Product]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27]

Whitaker,J.R.(1963),Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole. Analytical Chemistry35(12), 1950-1953[Positive Drive Turn Belt for Spiral Freezing Equipment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Yitai Die Making 13mm Beech Rotary PlywoodAuthor for correspondence