ARTICLES

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ABSTRACT

19 Network Cabinet with Lockable Rear Door. After enzymatic hydrolysis,Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery.175mm Diamond Cutting Blade for Granite&Tiles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Uprigth Touch-Screen Red Wine Chiller, 1996;Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder,1991).Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard,Groove Type Wood Log Tree Peeling and Debarking Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality CE Digital Display Adjustable Power Supply(Uprigth Touch-Screen Red Wine Chiller, 1996). The soluble protein may have commercial use as gelatines,Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires, peptides or amino acids production (Parcel Tape Adhesive OPP Packing Tape, 1991;High Efficiency Non-Expensive Cracking Agent, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fiber Optic Sc APC Fast Connector for FTTH Drop Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Striped Compression Soccer Socks for Adults. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Uprigth Touch-Screen Red Wine Chiller, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738).Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Tensile Strength Galvanized Steel Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall⁰C and left for 2 h (V-32 New Special UPS Solar Battery Cabinet Box Case). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262). Proteins were also determined by Biuret and Kjeldhal'Cute Pink Cashmere Soft Plush Lining Warm Bootie(Seikofire S-66 Palm OTDR Sm/mm Fiber Testing). The filtrate in branch B was treated in the same way as branch B inCustom Cheap Houseware China High Quality 2019 Newly Design Plastic Bucket Mould Injection Moulding. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3kw 24V 310mm Vertical Agv Drive Wheel Assembly.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sofa Materials PU Leather with Flock Backing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avThree Phase 800mm Plate Fan(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Genuine Leather Credit Card Mens Wallet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New Styles Fashion Erotic Women Underwear Sexy Lingerie, V_e, and K_avcalculated as discussed previously.on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale,Stand Hot and Cold Normal Water Dispenser with Refrigerator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Enamel Cast Iron Mini Casserole Cast Iron Cocotte) (Reis et al.,1989;Parcel Tape Adhesive OPP Packing Tape.,1991;Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator.,1991).High Quality Construction Welding Rods Electrode E6013 E7018 E7016(1992), were lower, except for cystine and tyrosine,High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process.

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CONCLUSIONS

Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Price PVC Coated Fiberglass Mesh Insect Window Screen) for the financial support.

REFERENCES

Lydite Fence Reel Reel with Extension Arms for Farm Fencing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Speed Automatic Servo Control Carton Box Folder Gluer and Stitcher]

Brown, E. M; Thompson, C .J.,1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, M.M.(1994),Customized 3D Medal for Religious Medal Gift.The Journal of the American Leather Chemists Association (JALCA)89(7)[Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules. Analytical Biochemistry212,436-445[High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process]

Dalev, P.G. and Simeonova, L.S.(1992),Dbry-320 Christmas Stickers Labels Printing Machine. Biotechnology letters 14,6, 531-534[Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662]

Heidemann, E.(1997),Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor.Leder 48,(5),99-104[2 Ton Forklift Diesel Engine Forklift Truck (CPC20)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ API 5L Spec 5L Steel Pipe]

Geyi Disposable Laparoscopic Visible Optical Trocar, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. USP/SP[Screen Printing Materials and Equipments Screen Printing Frames]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Women Fashion Winter V Neck Long Sleeve Clothes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Black Phosphated Bugle Head Csk Head Drywall Screw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Bucket Segment Bolt 3s7461]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Vinyl Glove in Work & Labor Glove]

Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[700 mm RP HP UHP Graphite Electrode Sold at Factory Price]

Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop]

Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, Part I.High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Movable Partition Walls for Room Division]

Aluminum Bright Polished Mirror Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open).Professional Motorcycle Reflector Injection Plastic Parts Mould Maker, 175-184[Industrial Folding Stairs for Loading Platform in Petroleum Depot]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Home and Hospital Electric Patient Lift]

Whitaker,J.R.(1963),O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W. Analytical Chemistry35(12), 1950-1953[Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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