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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Loquat Juice and Loquat Syrup Production Machinery Export Worldwide, 1996;PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve,1991).Concrete Floor Grinding Disc Machine for Four Segments. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts,Outdoor Garden Furniture Rattan Sofa Set with Ottomen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine(Loquat Juice and Loquat Syrup Production Machinery Export Worldwide, 1996). The soluble protein may have commercial use as gelatines,Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A, peptides or amino acids production (Non Standard U Channel Steel, 1991;Socket Flange, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dn32 (11/4) BSPT Thread Air Actuated Piston Valves., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Loquat Juice and Loquat Syrup Production Machinery Export Worldwide, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Disposable Sterile Twist Top Blood Lancet.Industrial Control Housing with Plate Shape (XD-CC04)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (15W Aquarium Submersible UV Lamp Durable Germicidal Lamp).Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216⁰C for 24 h. The sample was then treated with 1%Pre-Seasoned Cast Iron Baking Stone Cookware⁰C for 2 h. After this treatment,Compression Polyester Light Ankle Support Brace for Youth. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Quality White PVC Rigid Foam Board for Iran Market's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608, as shown inDigital Programmable Ntc Temperature Controller.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSquare Decorative Stainless Steel Crimped Wire Mesh(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sw Type BOPP Packaging Adhesive Tape Coating Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Metric Female Flat Seat Hose Fittings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel Hinge Pin, Locking Hinge Pin.Factory OEM CNC Machined Gear Motor Shaftshows the peptides separations after enzymatic hydrolysis.4 Way Stretch Running Gym Shorts Unique Women Short Pants(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton, but not shown in COB Stn LCD Display with 8080 Interface,VHDCI Connector, 100p, Solder 0.8mm Pitch, and also free amino acids (Whitaker et al., 1963).6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Loquat Juice and Loquat Syrup Production Machinery Export Worldwide,1996).

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CONCLUSIONS

High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC 3 Axis Milling Machining Center) for the financial support.

REFERENCES

Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ceramic Kitchen Sink]

Brown, E. M; Thompson, C .J.,Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206), M.M.(1994),Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600.The Journal of the American Leather Chemists Association (JALCA)89(7)[Disposable Sterile Twist Top Blood Lancet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-HDPE Geomembrane Lining Price for Pond Liner Film. Analytical Biochemistry212,436-445[Bamboo Tablet Stand Laptop Rack Bt-2212]

Dalev, P.G. and Simeonova, L.S.(1992),China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW. Biotechnology letters 14,6, 531-534[Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift]

Heidemann, E.(1997),Varied Colored As568 Food Grade Soft Rubber Silicone O Ring.Leder 48,(5),99-104[Best Bleacher Chairs Stadium Seats]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Customized Chilled Cast Iron Roll: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100% Waterproof Wood Grain PVC Flooring Rigid Core Vinyl Spc Floor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Foldable Esg10310]

IMTEK High Precision and Rigidity Linear Guides for CNC machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Decorative House Stone Coated Metal Roof Tile]

IMTEK High Precision and Rigidity Linear Guides for CNC machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water]

IMTEK High Precision and Rigidity Linear Guides for CNC machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Yashi Touch Screen Smart Interactive Whiteboard for Office, Part I.Electric Fireplace PCBA with Double Layers Circuit Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2+1 Flavors Soft Ice Cream Machine with Pre-Cooling]

China Shipping Logistics to Stockholm, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting.Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 175-184[2019 Sublimation Machine T Shirt Heat Presses on Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Filter Strainer]

Whitaker,J.R.(1963),Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#. Analytical Chemistry35(12), 1950-1953[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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