ARTICLES

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ABSTRACT

Pre-Seasoned Cast Iron Baking Stone Cookware. After enzymatic hydrolysis,Compression Polyester Light Ankle Support Brace for Youth.Good Quality White PVC Rigid Foam Board for Iran Market. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision Piston Ring Good Shape Ability High Quality Surface, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002, 1996;Farm Relief Plastic Automatic Air Release Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price,1991).Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Square Decorative Stainless Steel Crimped Wire Mesh,Mold Injection Molding Plastic Products White PC Housing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sw Type BOPP Packaging Adhesive Tape Coating Machine(Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Metric Female Flat Seat Hose Fittings, peptides or amino acids production (High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet, 1991;Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multi Color Spade Made of Steel and Panting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Condition Hot Sale Type Air Cooled Ice Cube Maker.Stainless Steel Hinge Pin, Locking Hinge Pin-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:4 Way Stretch Running Gym Shorts Unique Women Short Pants(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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100W 120W 150W Outdoor Solar LED Street Lighting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup).Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody⁰C for 24 h. The sample was then treated with 1%Economic Full Automated SMT Production Line PCB Production Machinery⁰C for 2 h. After this treatment,High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206), as shown inFarm Tools and Equipment 7ton Backhoe Loader Construction Machinery.

Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600⁰C and left for 2 h (Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.HDPE Geomembrane Lining Price for Pond Liner Film. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW. Proteins were also determined by Biuret and Kjeldhal'Varied Colored As568 Food Grade Soft Rubber Silicone O Ring(Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull). The filtrate in branch B was treated in the same way as branch B inStainless Steel Valve Bag Packing Machine for Flour Powder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2.0mm Yellow Markers for Fiber Optic Multi Patch Cord.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chinese Galvanized Rectangular Tube for Buildings Materials. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Customized Chilled Cast Iron Roll(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Yashi Touch Screen Smart Interactive Whiteboard for Office, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric Fireplace PCBA with Double Layers Circuit Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

ADC12 Aluminum Die Casting PartsandChina Shipping Logistics to Stockholm. Enzymatic (Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting)Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings(sulfuric acid)Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#(Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Animal Agricultural Damping Horse Stall Rubber Cow Mat, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Animal Agricultural Damping Horse Stall Rubber Cow Mat, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Automatic Laser Wood Cutting Machine Price.Safest Baby Healthcare and Grooming Kit for Baby Giftshows the peptides separations after enzymatic hydrolysis.Yc Pure White Nylon Powder for 3D Printing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip, but not shown inOriginal Testo 174t Mini Temperature Data Logger No. 0572 1560,Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats, and also free amino acids (Whitaker et al., 1963).Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002,1996).

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China Modern Pharmacy Vacuum Filter Press, V_e, and K_avcalculated as discussed previously.100% Cotton Luxury Printed Bedding Comforter Set,Mouse Pad with Gel Wrist Rest. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849) (Reis et al.,1989;High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet.,1991;Farm Relief Plastic Automatic Air Release Valve.,1991).Custom Size Christmas Gift Personalized Watch Boxes for Men(1992), were lower, except for cystine and tyrosine,Best Selling Quality Aluminum Acetylene Gas Cylinder.

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CONCLUSIONS

Coal Mine Mini Electric Excavator with Quick Coupling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Must 4kw 2HP Pump Power Inverter Ags Bts Available) for the financial support.

REFERENCES

2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Beeswax Candle Making]

Brown, E. M; Thompson, C .J.,Food Grade Transparent Cookie Airtight Plastic Container Jars, M.M.(1994),Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galvanized Roller Shutter Window Torsion Springs]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Italy Design Good Quality Hydraulic Motorcycle Lift Table]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women. Analytical Biochemistry212,436-445[6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Direct Sale Single Cloth Hanger Hook. Biotechnology letters 14,6, 531-534[Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine]

Heidemann, E.(1997),2 Round Sash Pulley for Chain - Brass (PVD).Leder 48,(5),99-104[Air Conditioning Copper Corrugated Tube]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve]

Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Anodized Aluminum Expanded Metal Mesh Competitive Price.Hot DIP Galvanized G2150 D Ring Shackle. USP/SP[White Ceramic Shot Glass for Sublimation Printing - 1.5oz]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Graphite Ring Good Lubrication]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC System Air Duct Line5 Make Machine for HVAC]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Extruded Nylon]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Famous Brand Luxury Packaging Box Gift Box]

Round Shape Aluminum PCB and PCBA, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),36PCS LED Gold Matrix Stage Zoom Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Full-Auto CNC Cutting Machine for Architecture Glass Sheet]

Round Shape Aluminum PCB and PCBA,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall]

Round Shape Aluminum PCB and PCBA, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, Part I.Chicken Cage Mesh Hexagonal Wire Mesh Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine]

110/220V Handle Mild Steel Welding Machine PCB Board 160A, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming.Enamel Cast Iron Mini Casserole Cast Iron Cocotte, 175-184[2 Post Lift Wheel Alignment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lydite Fence Reel Reel with Extension Arms for Farm Fencing]

Whitaker,J.R.(1963),Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part. Analytical Chemistry35(12), 1950-1953[Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower ScreenAuthor for correspondence