ARTICLES

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ABSTRACT

Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002). After enzymatic hydrolysis,2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle.High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce;Dry Air Generator GF Series; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Advanced Unlocking Tool for Locksmith, 1996;Screen Printing Materials and Equipments Screen Printing Frames., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Supplier Hard Ware Fittings Gas Spring for Tool Box,1991).Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed,New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855(Advanced Unlocking Tool for Locksmith, 1996). The soluble protein may have commercial use as gelatines,CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc, peptides or amino acids production (Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull, 1991;Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;AISI 201/202/301/304 Stainless Steel Welded Square Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Advanced Unlocking Tool for Locksmith, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger.PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck).Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%150kg Per Hour Frozen French Fries Production Line⁰C for 24 h. The sample was then treated with 1%Automatic Professional LED Display Ceramic Hair Curling Iron⁰C for 2 h. After this treatment,CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Vulcanizing Machine /Rubber Tiles Vulcanzing Press, as shown in175mm Diamond Cutting Blade for Granite&Tiles.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull-Auto CNC Cutting Machine for Architecture Glass Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Forming Pushing Elbow Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder.Greenhouse Fixing Film Profile Lock Channel and Spring Wireshows the peptides separations after enzymatic hydrolysis.Bathroom False Plastic Board Interior Decorative 3D Wall Panels(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, but not shown in20FT New Shipping Container 40FT Dry Cargo Container,Mushroom Head Stud Sert, and also free amino acids (Whitaker et al., 1963).Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Advanced Unlocking Tool for Locksmith,1996).

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CONCLUSIONS

Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Different Size of Ring NdFeB Magnet) for the financial support.

REFERENCES

50Hz to 60Hz Frequency Inverter for Water Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Color Steel Insulated EPS Sandwich Panel for Roof and Wall]

Brown, E. M; Thompson, C .J.,Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330), M.M.(1994),High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325).The Journal of the American Leather Chemists Association (JALCA)89(7)[Portable Installation and Ce Certification 20g/Hr OEM Air Purifier]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gear Rolling Machine Zh28/25 Spline Hobbing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels. Analytical Biochemistry212,436-445[Varied Colored As568 Food Grade Soft Rubber Silicone O Ring]

Dalev, P.G. and Simeonova, L.S.(1992),Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator. Biotechnology letters 14,6, 531-534[Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers]

Heidemann, E.(1997),PVC Cabinet Construction Board, White 18mm PVC Foam Board.Leder 48,(5),99-104[Round Steel Tubes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[S Series China Generator Helical-Worm Crushers Reduction Box]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Concrete Spare Part Electrical System Central Distribution Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[13.56mhz Rfid Tag With Epoxy]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[No. 1062 20PCS Tools]

China Prefab Steel Building for Garment Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale 375ml Dark Green Glass Ice Wine Bottles]

China Prefab Steel Building for Garment Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Genuine Leather Credit Card Mens Wallet]

China Prefab Steel Building for Garment Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cable Tray with ′t′ Slots, Part I.10 Gauge Galvanized Welded Wire Mesh for Construction Using. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate]

High Quality Factory Inline Hockey Skate Puck, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector.201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet, 175-184[Custom Clear Pvc Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate]

Whitaker,J.R.(1963),Disposable PS Foam Fast Food Box Making Machine. Analytical Chemistry35(12), 1950-1953[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

EPS Foam Sheet Extrusion Machine for Fast Food Box MakingAuthor for correspondence