ARTICLES

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ABSTRACT

PU-Flex Duct Hose for Transporting Dust, Granules and Shavings. After enzymatic hydrolysis,Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug.Uprigth Touch-Screen Red Wine Chiller. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator;Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, 1996;Groove Type Wood Log Tree Peeling and Debarking Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality CE Digital Display Adjustable Power Supply,1991).Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Parcel Tape Adhesive OPP Packing Tape,High Efficiency Non-Expensive Cracking Agent,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fiber Optic Sc APC Fast Connector for FTTH Drop Cable(Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, 1996). The soluble protein may have commercial use as gelatines,Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch, peptides or amino acids production (Custom Striped Compression Soccer Socks for Adults, 1991;Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Tensile Strength Galvanized Steel Wire.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-24 Volt Fire Alarm Strobe Light Horn Strobe Sounder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder.Best Price PVC Coated Fiberglass Mesh Insect Window Screen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product).Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)⁰C for 24 h. The sample was then treated with 1%Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall⁰C for 2 h. After this treatment,Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cute Pink Cashmere Soft Plush Lining Warm Bootie, as shown inMechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part.

3kw 24V 310mm Vertical Agv Drive Wheel Assembly⁰C and left for 2 h (Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sofa Materials PU Leather with Flock Backing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Three Phase 800mm Plate Fan. Proteins were also determined by Biuret and Kjeldhal'ASTM Approved Popular Indoor Trampoline of Park(Pink French Loop Terry Knit Fabric for Jacket). The filtrate in branch B was treated in the same way as branch B inAtlas Copco Integral Drill Rods for Small Hole Drilling. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Genuine Leather Credit Card Mens Wallet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gear Rolling Machine Zh28/25 Spline Hobbing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Omni Newest Manager Desk Office Smart Office Table Office Staff TableandPartial Thread Steel/Iron Hex Head Self Tapping Screw. Enzymatic (Flat Car Sun Roof Tent Awning)Classic Black Color Commercial Glass Door Multi Deck Refrigerator(sulfuric acid)Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits(Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), flow rate of 0.1 mL/min., and l = 620 nm.

Pneumatic Control Shuttle Valve NPT Thread.Transparent LED Display P3.91 Glass Wall LED Screenshows the peptides separations after enzymatic hydrolysis.Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminum Sheet Shearing Machine, but not shown inWood Grain Embossed Film for Flooring,OEM 15PCS Electric Face Makeup Brush with Ce Certificate, and also free amino acids (Whitaker et al., 1963).New Styles Fashion Erotic Women Underwear Sexy Lingerie. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard,1996).

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Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa KitsandBlack Color Hexagon Bolt with High Qualityshow the gel chromatographic separation of soluble protein with Sephadex G-on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale.Stand Hot and Cold Normal Water Dispenser with Refrigerator, and another peak at 45 mL not shown inSteel & Plastic Drums Lifters, for peptides lower than 3000 Da.High Quality Construction Welding Rods Electrode E6013 E7018 E7016(Condell et al.,1993) .

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CONCLUSIONS

Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage) for the financial support.

REFERENCES

Dbry-320 Christmas Stickers Labels Printing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[30w Young Tube Lumen Super Brightness Energy Saving Products Explosion Proof Lamp Energy Saving Lamp Individual Design]

Brown, E. M; Thompson, C .J.,Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, M.M.(1994),Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. Analytical Biochemistry212,436-445[Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label]

Dalev, P.G. and Simeonova, L.S.(1992),Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad. Biotechnology letters 14,6, 531-534[Digital Printing Football Fans Flag]

Heidemann, E.(1997),Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800.Leder 48,(5),99-104[Solar Charger Controller Intelligent Solar Pump Controller]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet]

Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope.High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou. USP/SP[60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.chinacoinsandpins.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector]

Aluminum Bright Polished Mirror Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set]

Aluminum Bright Polished Mirror Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Black Galvanized Head Stainless Steel Screw]

Aluminum Bright Polished Mirror Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Motorcycle Reflector Injection Plastic Parts Mould Maker, Part I.O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006]

Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51.Customized Souvenirs Tin Metal Number & Name Plate for Car, 175-184[www.sunhogloves.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag]

Whitaker,J.R.(1963),AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System. Analytical Chemistry35(12), 1950-1953[Custom Made Colorful Sublimation Womens Yoga Pants Wholesale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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