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ABSTRACT

Medical Human Body Thermometer. After enzymatic hydrolysis,Patent Product Solar Insect Killer Lamp, Made in Goldsun, China..Greenhouse Fixing Film Profile Lock Channel and Spring Wire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket, 1996;Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52,1991).China Supplier Granular Coal Based Activated Carbon Filter Meida. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Glass Cutting Table/CNC Glass Cutting Machine,12 Bottles Portable Home Ues Thermoelectric Wine Chiller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter(48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket, 1996). The soluble protein may have commercial use as gelatines,Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), peptides or amino acids production (Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap, 1991;Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve.Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard).New Swim Shorts Grey Mens Board Shorts with Mesh Liner(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control⁰C for 24 h. The sample was then treated with 1%Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)⁰C for 2 h. After this treatment,Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Marine Mooring Hardware, Anchor Chain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls, as shown inSmall Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization).

Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick⁰C and left for 2 h (Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.QJ-M Moving small manual brick making machine with low cost. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. Proteins were also determined by Biuret and Kjeldhal'Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set(Metal Gold Marathon Running Sport Medal for Promotion Gift). The filtrate in branch B was treated in the same way as branch B inChinese Dongfeng 3000L Vacuum Sewage Suction Truck. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Braiding Machine for Weaving Flexible Metal Hose.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Metal Button Crystal Shank Button for Garment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ABC-508 Brake System Cleaning and Fluid Exchanging Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F).Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Managementshows the peptides separations after enzymatic hydrolysis.Peb Steel Structure Building Materials H-Section I Beam Steel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMesh Bag Packing 20kg Pure White Garlic, but not shown inNew Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet,Aluminum Oxide Non Woven Polishing Wheel for Machine, and also free amino acids (Whitaker et al., 1963).CAT6A FTP Patch Panel (WD6A-012). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket,1996).

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ACP Aluminium Cmposite Panel Cladding Extrusions) for the financial support.

REFERENCES

Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Seamless Steel Pipe Astm A312 Tp304 Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Tct Circular Saw Blade for Grass Cutting, M.M.(1994),Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Picc Puncture Care Kit. Analytical Biochemistry212,436-445[Good Quality White PVC Rigid Foam Board for Iran Market]

Dalev, P.G. and Simeonova, L.S.(1992),Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth. Biotechnology letters 14,6, 531-534[Molasses Fuel Ethanol Distillation Plant]

Heidemann, E.(1997),AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.Leder 48,(5),99-104[Classical Gym and Commercial Fitness Equipmen Decline Bench]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Temperaturethermocouple Male and Female Plug: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Versatile Parachute Nylon Camping Hammock with Mosquito Netting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Non Stick Silicone Coated Fiberglass Cloth]

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34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mold Injection Molding Plastic Products White PC Housing]

34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, Part I.High Efficiency Fine Powder Vibrating Screener. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3*19.8 NRA G2 AISI420 Needle Roller for Bearing]

High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plant Stands & Accessories Glass Hanging Flower Pot Bracket.Wooden Frame Stretched Canvas, Painting Canvas, 175-184[Black Oxide Din912 Screw]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker]

Whitaker,J.R.(1963),Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine. Analytical Chemistry35(12), 1950-1953[500 Tons Hydraulic Power Double Action Press with Cushion]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sanhe FC-2 Poultry Air VentAuthor for correspondence