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ABSTRACT

Snsc Fd30 Side Shift New Diesel Forklift 3ton. After enzymatic hydrolysis,Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117).Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Laptop Bag College Men Backpack Leather Back Pack Bags;Pet Dog Grooming Rake Dematting Tool Shedding Comb; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Apparel Applique Round Patch School Club Embroidery Collar Patch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, 1996;CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror,1991).Automatic Food Metal Detector Machine for Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Multi-Functional Backpack Manual 52cc Brush Cutter,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine(Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, 1996). The soluble protein may have commercial use as gelatines,Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm, peptides or amino acids production (White Cotton Satin Hotel Duvet Cover (DPFB8055), 1991;Small Cleaning Brush ESD Anti Static Brush ESD Brush Black, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PAC /Poly Aluminium Chloride for Water Treatment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Sheet Shearing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electrical PV DC Solar Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tomato Bubble and High Pressure Washing Machine.Upholstery Ribbon Christmas Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Supplied High Strength Marine Anchor Chain, Parana State,Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube.Humidity Refrigeration Digital Controller Jsd-100+, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAngle Plastic Product Fiber Glass Reinforced FRP Pultrusion ProfilesandPortable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321).

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Elegant Europe Dessign Pillow Top Mattress(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor).8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts⁰C for 24 h. The sample was then treated with 1%Modern 4 Person Workstation with Computer Desk Modular Office Furniture⁰C for 2 h. After this treatment,High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Women Fashion Winter V Neck Long Sleeve Clothes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line, as shown inVariable 50Hz 22kw AC Drive 3 Phase Frequency Inverter.

3D CNC Router for Wood Cutting Machine⁰C and left for 2 h (Designed Steel Frame Factory Building). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Screens / Wedge Wire Filter Element. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Amazon Armor TPU Silicone Phone Case for Honor 10 Lite. Proteins were also determined by Biuret and Kjeldhal'EPS Foam Sheet Extrusion Machine for Fast Food Box Making(China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW). The filtrate in branch B was treated in the same way as branch B in5042 6063 T5 Compressed Air Aluminum Tubing and Fittings Powder Coated. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Stainless Steel Plate Flange, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Low Price Top Selling Fashion Summer Boy Sandals, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

By6-8-200-L Automatic Doypack Premade Pouch Packing MachineandEasy Operational Cup Cake Filling Machine. Enzymatic (Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger)Folding Play Mat Crawl Play Mat(sulfuric acid)Wooden Grain Perforated Panel for Metal Mesh Curtain Wall(Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1, flow rate of 0.1 mL/min., and l = 620 nm.

Folding  Platform Hand  Trolley .6 Inch Plastic Breathing Cap for The IBC Valveshows the peptides separations after enzymatic hydrolysis.Pump Flower Sprinkler with Automatic Watering System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBlack Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts, but not shown inChina Customized Chilled Cast Iron Roll,Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant, and also free amino acids (Whitaker et al., 1963).Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery,1996).

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PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to OperateandTct Circular Saw Blade for Grass Cuttingshow the gel chromatographic separation of soluble protein with Sephadex G-Low Carbon Heavy Galvanized Steel Wire.Long Distance Measurement Power Laser Digital Meter, and another peak at 45 mL not shown inYashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, for peptides lower than 3000 Da.Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer(Condell et al.,1993) .

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CONCLUSIONS

New Design Inclined Plane 18W LED Wall Washer Lighting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse) for the financial support.

REFERENCES

2ton R Type Electric Wire Rope Hoist with Single Speed, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bluetooth Care Rehab Tens Unit]

Brown, E. M; Thompson, C .J.,Stainless Steel Body Infrared Burner Good Selling Gas Stove, M.M.(1994),High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier.The Journal of the American Leather Chemists Association (JALCA)89(7)[Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mesh Bag Packing 20kg Pure White Garlic]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Adhesive CAS 9003-35-4 Phenolic Resin. Analytical Biochemistry212,436-445[New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet]

Dalev, P.G. and Simeonova, L.S.(1992),Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop. Biotechnology letters 14,6, 531-534[304 Stainless Steel Anti-Corrosion Welded Wire Mesh]

Heidemann, E.(1997),Manufacture Best Choice Liquid Flocculant for Ink Removal.Leder 48,(5),99-104[Ph Buffering Agents]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Gld Brand Padlock Brass Popular in 5 Continents  ]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Silicone Slap Bracelet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel]

FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Laser Cutting Ceramic]

FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Multi Parameter Small Digital Domestic Water Meter TDS Meter]

FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Foil PE Bottle Cap Induction Seal Liner, Part I.Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale]

Sophisticated Durable Membrane Panel/Panel with 3m Glue, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder.HDPE Polyethylene PE Pipe for Water Supply, 175-184[Push up Lace Bra Silicone Backless Strapless Women Sexy Lingerie]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall]

Whitaker,J.R.(1963),Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad. Analytical Chemistry35(12), 1950-1953[Good Quality White PVC Rigid Foam Board for Iran Market]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Molasses Fuel Ethanol Distillation PlantAuthor for correspondence