ARTICLES

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ABSTRACT

Bathroom False Plastic Board Interior Decorative 3D Wall Panels. After enzymatic hydrolysis,Mushroom Head Stud Sert.Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket;High Borosilicate Glass Baking Dish Set Clear; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Embossing Roller for Tissue Paper, 1996;Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates,1991).Colorful  Reflective Sticker Reflective Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Concrete Spare Part Electrical System Central Distribution Box,Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,50Hz to 60Hz Frequency Inverter for Water Pump(Embossing Roller for Tissue Paper, 1996). The soluble protein may have commercial use as gelatines,Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330), peptides or amino acids production (High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325), 1991;4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Cabinet Construction Board, White 18mm PVC Foam Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 New Portable Mini Dental X-ray Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Embossing Roller for Tissue Paper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ss Reduced Port 2000wog 1PC Screwed Ball Valve.China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Prefab Steel Building for Garment Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Disposable PS Foam Fast Food Box Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe).Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP⁰C for 24 h. The sample was then treated with 1%Foska Good Quality Plastic Stapler Set with Staples⁰C for 2 h. After this treatment,Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hotel SPA Swimming Pool, Sport Room, Bathroom Towel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Powerful Cleaning Colorful Scouring Pad for Kitchen Use, as shown inMetal Gold Marathon Running Sport Medal for Promotion Gift.

500t Plywood Hot Press Woodworking Machine (12-25 layers)⁰C and left for 2 h (FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.10ml Round Clear Nail Polish Bottle with Brush Cap. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full Automatic 4000b/h 500ml Plastic Jar Making Machine. Proteins were also determined by Biuret and Kjeldhal'Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine(Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management). The filtrate in branch B was treated in the same way as branch B inContrast Color Workwear. The final soluble protein shown were used for peptides separations using gel permeation chromatography,5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHermetic Sliding Lead Door, X Ray Room Door(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.15W Aquarium Submersible UV Lamp Durable Germicidal Lamp⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Accept OEM New Products Team Various Colors Gold Ribbon, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine.New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Walletshows the peptides separations after enzymatic hydrolysis.Home and Hospital Electric Patient Lift(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in304 Stainless Steel Anti-Corrosion Welded Wire Mesh, but not shown inFrameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware,Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, and also free amino acids (Whitaker et al., 1963).M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Embossing Roller for Tissue Paper,1996).

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Medical Human Body Thermometer, V_e, and K_avcalculated as discussed previously.Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.,Greenhouse Fixing Film Profile Lock Channel and Spring Wire. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Multi Parameter Small Digital Domestic Water Meter TDS Meter) (Reis et al.,1989;High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325).,1991;Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel.,1991).30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products(1992), were lower, except for cystine and tyrosine,Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle.

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CONCLUSIONS

RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (10 Gauge Galvanized Welded Wire Mesh for Construction Using) for the financial support.

REFERENCES

48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Free sample Full Automatic Compost Fertilizer Production Line - Vertical Fertilizer Chain Crusher - Exceed ]

Brown, E. M; Thompson, C .J.,Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, M.M.(1994),Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Quality White PVC Rigid Foam Board for Iran Market]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Molasses Fuel Ethanol Distillation Plant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier Granular Coal Based Activated Carbon Filter Meida. Analytical Biochemistry212,436-445[Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable]

Dalev, P.G. and Simeonova, L.S.(1992),Glass Cutting Table/CNC Glass Cutting Machine. Biotechnology letters 14,6, 531-534[SMD LED Light-Operated USB Rechargeble Flashlight]

Heidemann, E.(1997),12 Bottles Portable Home Ues Thermoelectric Wine Chiller.Leder 48,(5),99-104[China Caco3 Plastic Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)]

200W UFO LED High Bay Light Fixture for Interior Exterior Applications, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter.Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321). USP/SP[Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Non Stick Silicone Coated Fiberglass Cloth]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[40A C Electronic Air Circuit Breaker with Dz47-4p]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vibratory Finishing Machine For Remove Burrs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mold Injection Molding Plastic Products White PC Housing]

Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3*19.8 NRA G2 AISI420 Needle Roller for Bearing]

Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker]

Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe, Part I.Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500 Tons Hydraulic Power Double Action Press with Cushion]

Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron.Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, 175-184[Office Plastic Floor Commercial Grade Sheet Vinyl Flooring]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sanhe FC-2 Poultry Air Vent]

Whitaker,J.R.(1963),High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt. Analytical Chemistry35(12), 1950-1953[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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