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ABSTRACT

Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans. After enzymatic hydrolysis,18V Lithium Battery Powered Cordless Drill.High Density Rock Wool Wall Board with Thermal Insulation Performance. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China PAC Water Flocculating Agent for Water Treatment, 1996;Factory Price High Carbon Isostatic Graphite Electrodes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets,1991).Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light,Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Powder/Spices Powder Packing Machine with Four/Three Side Seal(China PAC Water Flocculating Agent for Water Treatment, 1996). The soluble protein may have commercial use as gelatines,Black Phosphated Bugle Head Csk Head Drywall Screw, peptides or amino acids production (Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, 1991;China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6 Inch Plastic Breathing Cap for The IBC Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China PAC Water Flocculating Agent for Water Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging.Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rechargeable Battery Operated Cooling Folding Electric Fan(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Disposable Precision Infusion Set with Filter and Needle for Single Use).High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%21.5′′ Wall Mount Display Capacitive Touch Monitor⁰C for 24 h. The sample was then treated with 1%Pagoda Gazebo Tent in Guangzhou for Sale⁰C for 2 h. After this treatment,Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Black Small Fridge for Showcase, as shown inYcom USB Charger and Data Cable with Aluminum for Type C.

Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)⁰C and left for 2 h (Anodized Gold Brush Finish Aluminum Sheet Coil). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Zircon Butterfly Open Ring Diamond Fashion Woemn Rings. Proteins were also determined by Biuret and Kjeldhal'High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation(Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed). The filtrate in branch B was treated in the same way as branch B inCarbon Graphite Electrode. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Spare Parts Custom Injection Plastic Moulding Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Speed Roll Plastic T-Shirt Bag Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly ValveandWholesale Black Galvanized Head Stainless Steel Screw. Enzymatic (PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine)45*60cm Anti-Fatigue ESD Rubber Floor Mat(sulfuric acid)Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints(China PAC Water Flocculating Agent for Water Treatment, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Vinyl Glove in Work & Labor Glove, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Vinyl Glove in Work & Labor Glove, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

1220*2440mm Concrete Formwork black film faced plywood. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer) for the financial support.

REFERENCES

Washami Teeth Care for Whitening Cool Mint Toothpaste, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gas-Liquid Separator]

Brown, E. M; Thompson, C .J.,Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, M.M.(1994),Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[3.0kw Spindle CNC Router with Certificate for Woodworking]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SHXJ1500 CNC Diamond Wire Stone Cutting Machine. Analytical Biochemistry212,436-445[Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron]

Dalev, P.G. and Simeonova, L.S.(1992),Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag. Biotechnology letters 14,6, 531-534[Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace]

Heidemann, E.(1997),2019 Foam Concrete Machine/Foamed Concrete Block Making Machine.Leder 48,(5),99-104[Tactile Dustproof Touch Screen Push Button Keyboard Membrane Switch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower]

High Power From Han′s GS 2000W Laser Cutter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball.Yyh Series One Dimension Tumbling Mixer Mixing Machine. USP/SP[Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Aluminum Heat Sink for Electronics Computer Electric]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ODU 14 Pin Industrial Circular Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Blood Cuff For Patient Monitor High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag]

Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Classical New Coming Plastic Female Sunglasses-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset]

Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)]

Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Double Sided Rigid PCB Circuit Board Manufacturer, Part I.Robot of World Robotics Competition. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station]

Professional Rope/Cable/Wire Braiding Machine with Factory Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum PCB Circuit Board SMD PCBA LED PCB.Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159), 175-184[Double Bevel Curve Blades]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use]

Whitaker,J.R.(1963),Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck. Analytical Chemistry35(12), 1950-1953[Snsc Fd30 Side Shift New Diesel Forklift 3ton]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Glass Mix Stone Mosaic TilesAuthor for correspondence