ARTICLES

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BiomassEdivaldo E.Garcia^I; Cláudia C.M.Cog Graphic FPC LCD Display Module^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Power Fitting- Hot DIP Galvanizing Bolt with Nut. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Slewing Gear for Tower Crane Slewing Winch Reducer

ABSTRACT

Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine. After enzymatic hydrolysis,7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side.High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System;Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, 1996;Single Screw Extruder for Plastic PP/PE Pipe Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck,1991).Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Gold Star Square Glass Candle Holder for Wedding Decoration,11kg 24L Low Pressure LPG Gas Cylinder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Commercial Use Click Lock Standard Spc Vinyl Plank Flooring(Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, 1996). The soluble protein may have commercial use as gelatines,Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, peptides or amino acids production (High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, 1991;Precision Customized OEM Auto Hardware Lathe CNC Machining Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;White Color Enamel Bowls with High Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board.Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Southeast Asia Healthy Soft Integrated Latex Foam Mattress(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing PadAlcalase2.5 L DX,Water Filter System Lamella Clarifier for River Water Treatment System, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Cabinet Shelf Support Pin Furniture Hardware Fittings(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Reasonable Price High Quality Nylon Cable Gland, Parana State,Slewing Gear for Tower Crane Slewing Winch Reducer.Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/StoneandAnodized Aluminum Expanded Metal Mesh Competitive Price.

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Disposable Anesthesia Breathing Circuit Tube(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Toilet Roll Tissue Wrapping Machine with Auto - Counting).High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element⁰C for 24 h. The sample was then treated with 1%New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126⁰C for 2 h. After this treatment,Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Counter-Clip Fluorine-Lined Butterfly Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)DC Brushless Motor for CNC Machine with ISO9001, as shown in Painting Canvas.

Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck⁰C and left for 2 h (High Quality Industrial Grade Anhydrous Sodium Sulfate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Road Safety Warning Barricade LED Solar Powered Blinking Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Manufacturer Customized Plastic Injection Molding. Proteins were also determined by Biuret and Kjeldhal'Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)( Safe Lock (AL-206)). The filtrate in branch B was treated in the same way as branch B inIndustrial Grade Caustic Soda 99%. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Aluminum Cover Frame Half Aluminum Cover Profile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book ShelfandDC24V 36W Solar Fan Motor PCBA Card. Enzymatic (New Monocular Hunting Wargame Mini Scope Digital Night Vision)Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine(sulfuric acid)Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor(Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Italy Design Good Quality Hydraulic Motorcycle Lift Table, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Italy Design Good Quality Hydraulic Motorcycle Lift Table, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale Menthol E Liquid E Juice for EGO E Cigarettes.Low Carbon Heavy Galvanized Steel Wireshows the peptides separations after enzymatic hydrolysis.Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in50Hz to 60Hz Frequency Inverter for Water Pump, but not shown inEmbossing Waterproof Push Button Membrane Switch Panel Membrane Keypad,New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9, and also free amino acids (Whitaker et al., 1963).Portable Telescopic Straw Metal Drinking Straw with Brush Case. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved,1996).

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Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, V_e, and K_avcalculated as discussed previously.CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy,Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill) (Reis et al.,1989;High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.,1991;Single Screw Extruder for Plastic PP/PE Pipe Production Line.,1991).Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001)(1992), were lower, except for cystine and tyrosine,Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making.

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CONCLUSIONS

Double Roller Rotary Wood Peeling Machine with CE for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)) for the financial support.

REFERENCES

5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tx150/Mini Tractor/12HP/14HP/15HP/16HP/18HP/20HP/Tx150/Wheel Tractor/2WD/Belt-Driven Tractor]

Brown, E. M; Thompson, C .J.,Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), M.M.(1994),Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tube Mills for Thin Wall Tubing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Lishui Factory High Quality Metal Rolling Ball Screw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ladle Refining Melting Steel Furnace Casting Ladle. Analytical Biochemistry212,436-445[2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt]

Dalev, P.G. and Simeonova, L.S.(1992),Lishui Factory High Quality Metal Rolling Ball Screw. Biotechnology letters 14,6, 531-534[Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling]

Heidemann, E.(1997),Y-Spring Threaded Check Valve, 800 Wog.Leder 48,(5),99-104[8 Inch Ro Pressure Vessel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Zircon Butterfly Open Ring Diamond Fashion Woemn Rings]

Cog Graphic FPC LCD Display Module, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part.Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle. USP/SP[Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Galvanized Eye Grab Bend Hook: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[60ml 100ml Customized Glass Jar for Spice]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Jy 10 Heads Multihead Combination Scale with Vffs Packing Machine for Chocolate Beans]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent]

Transparent LED Display P3.91 Glass Wall LED Screen, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Surgical Power Tool Medical Care Orthopedic Mini Bone Saw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Humidity Refrigeration Digital Controller Jsd-100+]

Transparent LED Display P3.91 Glass Wall LED Screen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)]

Transparent LED Display P3.91 Glass Wall LED Screen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Micro Small Plastic Product Injection Molding Making Machine, Part I.Glass Lifting Machine with Sucker for Glass Movements. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Nylon Weatherproof PA6 Strain Relief Cable Gland]

Anodized Gold Brush Finish Aluminum Sheet Coil, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Popular Industrial Sewing Quilting Machine With CO.Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace, 175-184[Composite Laid Scrims Mesh]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum Bright Polished Mirror Sheet]

Whitaker,J.R.(1963),Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate. Analytical Chemistry35(12), 1950-1953[Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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