ARTICLES

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www.wzzy-spring.comEdivaldo E.Garcia^I; Cláudia C.M.Micro Small Plastic Product Injection Molding Making Machine^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Outdoor Garden Save Energy with Solar Flame Wall Light}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Glass Lifting Machine with Sucker for Glass Movements. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Anodized Gold Brush Finish Aluminum Sheet Coil

ABSTRACT

Popular Industrial Sewing Quilting Machine With CO. After enzymatic hydrolysis,Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace.Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pure Sine Wave Power Frequency Inverter;Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Colorful 100% Cotton Hotel Face Towels, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S), 1996;33kv Dry Type Resin Casted Distribution Transformer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27,1991).Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Size 000 Clear Pullulan Vegetarian Capsules,Sinicline 2018 Hot Sell Custom Printing Thank You Cards,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Black Red Open Net Spandex Nylon Sexy Lingerie(High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S), 1996). The soluble protein may have commercial use as gelatines,7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting, peptides or amino acids production (Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor, 1991;LED Emergency Exit Lights, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY.3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hardware CNC Machinery Milling Part, Parana State,Anodized Gold Brush Finish Aluminum Sheet Coil.Customized 8 Layers Round LED Panel Aluminum PCB for LED Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDaily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal PowderandMultifunctional Solar Powered Safety Hammer LED Flashlight.

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Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag).Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)⁰C for 24 h. The sample was then treated with 1%Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape⁰C for 2 h. After this treatment,Linear Guideway for CNC Machine as Components. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), as shown inNatural Latex Pocket Coil Spring Mattress for 5 Star Hotel.

Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r⁰C and left for 2 h (Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. Proteins were also determined by Biuret and Kjeldhal'External Retaining Ring for Shaft, DIN471, D1400(Steel Forged Automobile Spare Parts with Customized Drawing). The filtrate in branch B was treated in the same way as branch B inPlastic Corrosion Resistance of CNC Machining Parts Processing Machined Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRotary Vibrating Screen/Sieve/Sifter/Griddle Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Rubber Product Seal O-Ring /NBR70 O Ring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fully Automatic Dust Face Mask Making Machine with Ultrasonic, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning MachineandMuslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf. Enzymatic (460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle)Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag(sulfuric acid)New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints(High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%DIN 7337 Aluminium Core Pulling Stainless Steel Rivet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%DIN 7337 Aluminium Core Pulling Stainless Steel Rivet, flow rate of 0.1 mL/min., and l = 620 nm.

Professional Bb Trumpet (ATR6506). 100pshows the peptides separations after enzymatic hydrolysis.Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, but not shown inChinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale,3.5ton Chinese Engine Gasoline Forklift (FG35T), and also free amino acids (Whitaker et al., 1963).Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S),1996).

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Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300Wand8oz 250ml Disposable Paper Soup Bowl with Lid for Take Awayshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle.Waterproof Covering Material Interior Wall Composite Panel, and another peak at 45 mL not shown in14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna, for peptides lower than 3000 Da.Tall/Large Cube Glass Terrarium Planter Gift Card Holder(Condell et al.,1993) .

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Venlo Type Flower Multi Span Commercial Glass Greenhouses Used, V_e, and K_avcalculated as discussed previously.XPS Extruded Polystyrene Foam Board,Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap) (Reis et al.,1989;Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor.,1991;33kv Dry Type Resin Casted Distribution Transformer.,1991).Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth(1992), were lower, except for cystine and tyrosine,Kh No Fried Automatic Potato Chips Making Machines.

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CONCLUSIONS

Deep Hole Cylinder Boring Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump) for the financial support.

REFERENCES

Good Prize Solar PV Mounting Panel System for Ground, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

Brown, E. M; Thompson, C .J.,Electric Heavy Duty Rail Guided Vehicle for Transportation, M.M.(1994),Sower Vacuum Emulsifying Machine for Cosmetic.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ASTM A240 309s Stainless Steel Sheet. Analytical Biochemistry212,436-445[Southeast Asia Healthy Soft Integrated Latex Foam Mattress]

Dalev, P.G. and Simeonova, L.S.(1992),Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home. Biotechnology letters 14,6, 531-534[Classical New Coming Plastic Female Sunglasses]

Heidemann, E.(1997),Car Mattress Back Inflatable Air Bed Car.Leder 48,(5),99-104[Solar Outdoor Traffic LED Variable Message Sign]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type]

Micro Small Plastic Product Injection Molding Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum.Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine. USP/SP[Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Single Embossed Buttons Membrane Switch with FPC Circuit: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Barb Offtake Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses]

3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Popular Laser Cutting Engraving Machine for Nonmetals-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Children Educational Toys Game]

3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture]

3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, Part I.Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Bearing with Housing Bearing / Pillow Block Bearing]

Large Slewing Rings Bearings for Wind Turbine Ring on Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container.Chemicals Flocculant Cationic Polymer Price, 175-184[Double Screw 2 Color Plastic Film Extruder Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Milling Precision Stainless Steel CNC Machinery Parts]

Whitaker,J.R.(1963),Ycom USB Charger and Data Cable with Aluminum for Type C. Analytical Chemistry35(12), 1950-1953[Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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