ARTICLES

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^IICNPq/RHAE/biotechnology-Professional Bb Trumpet (ATR6506). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines

ABSTRACT

3.5ton Chinese Engine Gasoline Forklift (FG35T). After enzymatic hydrolysis,Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover.Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Waterproof Covering Material Interior Wall Composite Panel;Tall/Large Cube Glass Terrarium Planter Gift Card Holder; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Venlo Type Flower Multi Span Commercial Glass Greenhouses Used, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (XPS Extruded Polystyrene Foam Board, 1996;Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth,1991).Kh No Fried Automatic Potato Chips Making Machines. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Deep Hole Cylinder Boring Machine,Good Prize Solar PV Mounting Panel System for Ground,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electric Heavy Duty Rail Guided Vehicle for Transportation(XPS Extruded Polystyrene Foam Board, 1996). The soluble protein may have commercial use as gelatines,Sower Vacuum Emulsifying Machine for Cosmetic, peptides or amino acids production (ASTM A240 309s Stainless Steel Sheet, 1991;Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Car Mattress Back Inflatable Air Bed Car., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (XPS Extruded Polystyrene Foam Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Single Embossed Buttons Membrane Switch with FPC Circuit.3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Popular Laser Cutting Engraving Machine for Nonmetals(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Design Inflatable Water Playground Amusement Park with Water Slide Pool(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Double Roller Rotary Wood Peeling Machine with CE for Sale).240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Light Weight Polyester Eye Mask for Travel Airplane Hotel⁰C for 24 h. The sample was then treated with 1%6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)⁰C for 2 h. After this treatment,2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bamboo Tablet Stand Laptop Rack Bt-2212's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book, as shown inLightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter.

Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair⁰C and left for 2 h (Factory Price High Carbon Isostatic Graphite Electrodes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Personal Care Glutathione Skin Whitening Tablets. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Over Size Boys Hoodie with Embroidered. Proteins were also determined by Biuret and Kjeldhal'Digital Printing Football Fans Flag(A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve). The filtrate in branch B was treated in the same way as branch B inOutdoor Full Color LED Display Signs for Advertisement with High Refresh Rate (pH10 960mm*960mm). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Promotional Plastic Laminated Custom Shopping PP Woven Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Galvanized Wave Form Perforated Steel Strips, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

E171 Standrad Titanium Dioxide Food Grade A200andMelamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12). Enzymatic (Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay)Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale(sulfuric acid)Silver Anodized Aluminium I Beam Level Measuring Tool(XPS Extruded Polystyrene Foam Board, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, flow rate of 0.1 mL/min., and l = 620 nm.

48V25A Lead Acid Battery Maintenance Pulse Charger.High Efficiency Non-Expensive Cracking Agentshows the peptides separations after enzymatic hydrolysis.15mm Best Price Commercial Grade Birch Plywood for Furniture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inVenlo Type Flower Multi Span Commercial Glass Greenhouses Used, but not shown inWhat′s Kind of Machine for Making Organic Fertilizer Granules,Top Great Wallet Genuine Leather Purse with Coin Pocket, and also free amino acids (Whitaker et al., 1963).Good Price 20kVA Single Phase Silent Diesel Generator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (XPS Extruded Polystyrene Foam Board,1996).

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CONCLUSIONS

Carrier Roller for Sany Excavator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Large Slewing Rings Bearings for Wind Turbine Ring on Sale) for the financial support.

REFERENCES

LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tripeptide-10 Citrulline]

Brown, E. M; Thompson, C .J.,Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, M.M.(1994),Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Eyes- Caring LED Night Light for Nursing Reading with Soft Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets. Analytical Biochemistry212,436-445[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002). Biotechnology letters 14,6, 531-534[Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel]

Heidemann, E.(1997),2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle.Leder 48,(5),99-104[Artificial Grass Sport Floor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Corrugated Board Production Line]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dry Air Generator GF Series: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Safe Durable Construction Scaffolding Plank for Building]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Auto Parts Outsourcing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board]

Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Advanced Unlocking Tool for Locksmith-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)]

Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup]

Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Screen Printing Materials and Equipments Screen Printing Frames, Part I.China Supplier Hard Ware Fittings Gas Spring for Tool Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery]

Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed.New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet, 175-184[jb-hardware]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant]

Whitaker,J.R.(1963),Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855. Analytical Chemistry35(12), 1950-1953[Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ADC12 Aluminum Die Casting PartsAuthor for correspondence